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Rat matrix metalloproteinase 1(MMP-1/Collagenase I) ELISA kit

  • 中文名稱:
    大鼠基質(zhì)金屬蛋白酶1(MMP-1/Collagenase I)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E07416r
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠基質(zhì)金屬蛋白酶1(MMP-1/Collagenase I)酶聯(lián)免疫試劑盒(CSB-E07416r)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的MMP1含量。MMP1,即基質(zhì)金屬蛋白酶1,是一種鋅依賴性內(nèi)肽酶,參與細(xì)胞外基質(zhì)(ECM)的降解,與細(xì)胞遷移、炎癥和腫瘤侵襲等生理和病理過(guò)程密切相關(guān)。MMP1通過(guò)降解ECM中的膠原和蛋白聚糖,改變細(xì)胞微環(huán)境,促進(jìn)腫瘤的侵襲和轉(zhuǎn)移。研究MMP1的機(jī)制有助于開(kāi)發(fā)針對(duì)腫瘤侵襲和轉(zhuǎn)移的治療策略。試劑盒檢測(cè)范圍為0.235 ng/ml- 15 ng/ml,靈敏度為0.058 ng/ml。適用于炎癥模型、組織工程研究、纖維化疾病機(jī)制探索等科研場(chǎng)景,能夠?yàn)樾难芗膊 ㈥P(guān)節(jié)炎等動(dòng)物模型的分子機(jī)制研究提供可靠數(shù)據(jù)支持本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    Mmp1Interstitial collagenase ELISA Kit; EC 3.4.24.7 ELISA Kit; Fibroblast collagenase ELISA Kit; Matrix metalloproteinase-1 ELISA Kit; MMP-1 ELISA Kit; Myocardial collagenase ELISA Kit; Fragment ELISA Kit
  • 縮寫(xiě):
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    0.235 ng/ml - 15 ng/ml
  • 靈敏度:
    0.058 ng/ml
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Cancer
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-1/Collagenase?I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    89

    Range %

    85-94

    1:2

    Average %

    92

    Range %

    87-98

    1:4

    Average %

    95

    Range %

    90-102

    1:8

    Average %

    100

    Range %

    96-109

  • 回收率:

    The recovery of rat MMP-1/Collagenase?I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    84

    80-89

    EDTA plasma (n=4)

    97

    90-104

  • 標(biāo)準(zhǔn)曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    15

    2.257

    2.231

    2.244

    2.147

    7.5

    1.876

    1.832

    1.854

    1.757

    3.75

    1.381

    1.373

    1.377

    1.280

    1.88

    0.835

    0.797

    0.816

    0.719

    0.94

    0.397

    0.376

    0.387

    0.290

    0.47

    0.273

    0.257

    0.265

    0.168

    0.235

    0.197

    0.207

    0.202

    0.105

    0

    0.099

    0.094

    0.097

     

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    MMP1,全名為基質(zhì)金屬蛋白酶-1,是一種廣泛存在于人體組織中的酶類分子。MMP1主要參與膠原蛋白的降解和清除過(guò)程,在組織再生、炎癥、腫瘤轉(zhuǎn)移等多種生理和病理過(guò)程中發(fā)揮重要作用。最近的研究表明,MMP1在多種癌癥的發(fā)生和發(fā)展中發(fā)揮關(guān)鍵作用,成為了一個(gè)重要的治療靶點(diǎn)。此外,MMP1還與關(guān)節(jié)炎、動(dòng)脈粥樣硬化等疾病的發(fā)生和發(fā)展密切相關(guān),成為了重要的生物標(biāo)志物和疾病預(yù)測(cè)因子。針對(duì)這些酶的抑制劑已成為研究的熱點(diǎn),可以用于疾病的預(yù)防和治療。
  • 功能:
    Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. May play a role in the deterioration of the heart wall extracellular matrix proteins during the onset of dilated cardiomyopathy.
  • 亞細(xì)胞定位:
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族:
    Peptidase M10A family
  • 數(shù)據(jù)庫(kù)鏈接:

    UniGene: Rn.79007



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