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  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Rat luteinizing hormone (LH) Elisa kit
  • 中文名稱(chēng)：
    大鼠黃體生成素（LH）酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12654r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠黃體生成素（LH）酶聯(lián)免疫試劑盒（CSB-E12654r）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的LH含量。LH即促黃體生成素，是腺垂體分泌的糖蛋白激素。它在生殖系統(tǒng)中扮演重要角色，可刺激卵巢排卵和黃體形成，促進(jìn)雄激素合成與分泌。研究表明，其水平變化與多種生殖內(nèi)分泌疾病相關(guān)，深入研究其作用機(jī)制有助于疾病診斷與治療。試劑盒檢測(cè)范圍為0.3 mlU/ml-60 mlU/ml，適用于科研領(lǐng)域探索生殖系統(tǒng)疾病模型建立、動(dòng)情周期激素動(dòng)態(tài)監(jiān)測(cè)、藥物干預(yù)對(duì)性腺軸的影響等研究場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    LhbLutropin subunit beta ELISA Kit; Lutropin beta chain ELISA Kit; Luteinizing hormone subunit beta ELISA Kit; LH-B ELISA Kit; LSH-B ELISA Kit; LSH-beta ELISA Kit
  • 縮寫(xiě)：
    LH
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型：
    serum, plasma
  • 檢測(cè)范圍：
    0.3 mlU/ml-60 mlU/ml
  • 靈敏度：
    0.15 mlU/ml
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線(xiàn)性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 88-100  
    1:2 Average % 95  
    Range % 91-101  
    1:4 Average % 94  
    Range % 85-105  
    1:8 Average % 96  
    Range % 90-102  
  • 回收率：
    The recovery of rat LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 93-107  
    EDTA plasma (n=4) 96 91-103  
                 
                 
  • 標(biāo)準(zhǔn)曲線(xiàn)：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average    
    60 0.150 0.153 0.152    
    15 0.352 0.338 0.345    
    4.8 0.513 0.549 0.531    
    1.2 0.985 1.002 0.994    
    0.3 1.476 1.441 1.459    
    0 2.679 2.654 2.667    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類(lèi)型：血清

樣品信息：大鼠

稀釋比：沒(méi)有稀釋

產(chǎn)品評(píng)價(jià)： 我用CSB-E12654r檢測(cè)正常(LH)血清,標(biāo)準(zhǔn)曲線(xiàn)的OD值為0.2562 0.5844 0.8108 1.1754 1.512 2.8934 ，樣本的OD值為為0.5-0.65

By 付老師

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    促黃體生成素是由垂體前葉分泌的蛋白質(zhì)激素，對(duì)于女性生殖周期中的排卵和黃體生成起重要作用。最新的研究發(fā)現(xiàn)，LH還與男性生殖健康密切相關(guān)，可促進(jìn)精子發(fā)生和成熟，并在精液中發(fā)揮重要作用。
  • 功能：
    Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
  • 基因功能參考文獻(xiàn)：
    1. Orchestrated Egr1 and Pitx1 activities depending on specific central and peripheral regulatory inputs could be responsible for physiologically variable Lhb gene promoter activation in vivo. PMID: 25258388
    2. GnRH stimulates AMPK, and AMPK inhibition suppresses GnRH-stimulated LHbeta transcription. PMID: 23518923
    3. data suggest that the molecular decision for LHbeta subunit to enter the regulated secretory pathway is a pre-Golgi event controlled by the C-terminal heptapeptide. PMID: 23734233
    4. increased LH concentration induces production of incomplete LHR transcripts in hippocampal cells and decreases immature LHR at the protein level PMID: 23678542
    5. Data suggest that LH beta subunit expression (and FSH beta subunit expression) is regulated by DUSP1 expression (specifically in response to high-frequency GnRH pulses); this effect may occur in association with MAPK3/1 phosphorylation. PMID: 21228211
    6. Complex interactions between Ahr and Esr alter Prl and luteinizing hormone (LH) synthesis by direct actions in lactotropes and gonadotropes. PMID: 21187122
    7. LHbeta promoter activity is further regulated by the orphan nuclear receptors, chicken ovalbumin upstream promoter-transcription factors (COUP-TFI and COUP-TFII). PMID: 20797425
    8. data suggest that LHbeta G1502A polymorphism may be involved in the predisposition to infertility and minimal/mild endometriosis-associated infertility, although endometriosis might be only a coincidental finding along with infertility. PMID: 20430510
    9. PACAP may be released from the anterior pituitary cells in a concentration which would be able to influence LH release not only in vitro but under in vivo conditions as well. PMID: 12409218
    10. These results suggest that GnRH directly regulates LH secretion as well as LHbeta-subunit synthesis, and that LH2 cells are a useful model for the study of LH secretion induced by several secretagogues. PMID: 12940464
    11. androgens acting directly on the pituitary increase FSH-beta and decrease LH-beta transcription PMID: 14512439
    12. identify a second functional Pitx1 region spanning positions -73 to -52 in the LHbeta gene promoter PMID: 16087728
    13. JNK regulates both LHbeta and FSHbeta transcription in a differential manner. PMID: 17932215
    14. LHbeta, FSHbeta and the common alpha-subunit mRNA's were detected in intact follicles, theca cells, corpora lutea and in meiotically competent and incompetent oocytes. PMID: 18248883
    15. the carboxyl-terminal sequence in the lutropin beta subunit contributes to the sorting of lutropin to the regulated pathway PMID: 18292086
    16. In the 14-day-old rat pituitary, the majority of GHRH target cells are cells expressing LH beta mRNA alone or in combination with GH and/or POMC mRNA. PMID: 18363807
    17. Results demonstrate that degradation via the proteasome is vital to GnRH-stimulated LHbeta expression, and this occurs in part by allowing proper transcription factor associations with the LHbeta promoter. PMID: 19095772
    18. Lhb gene transcription in rat pituitary requires GnRH-induced early growth response 1 gene expression, which is mediated by MAPK8/9 and MAPK1/3. PMID: 19710510

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Glycoprotein hormones subunit beta family
  • 數(shù)據(jù)庫(kù)鏈接：


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