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Rat lipoprotein lipase,LPL ELISA Kit

  • 中文名稱:
    大鼠脂蛋白脂酶(LPL)酶聯免疫試劑盒
  • 貨號:
    CSB-E08494r
  • 規格:
    96T/48T
  • 價格:
    ¥3900/¥2500
  • 其他:

產品詳情

  • 產品描述:
    大鼠脂蛋白脂酶(LPL)酶聯免疫試劑盒(CSB-E08494r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的LPL含量。LPL(脂蛋白脂肪酶)是一種由多種組織產生的多功能酶,主要參與脂質和脂蛋白代謝。其研究機制涉及催化乳糜微粒和極低密度脂蛋白中的甘油三酯水解,產生脂肪酸和單酰甘油,供組織利用。LPL基因突變可能導致脂質代謝紊亂,與多種疾病如2型糖尿病、高血壓、動脈硬化等關聯。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL,廣泛用于基礎科研領域,例如在動物模型中探究飲食干預、基因修飾或藥物處理對脂代謝通路的影響,評估不同生理病理狀態下LPL的活性變化及其與代謝綜合征的關聯機制,為心血管疾病、糖尿病等代謝相關疾病的機制研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Lpl ELISA Kit; Lipoprotein lipase ELISA Kit; LPL ELISA Kit; EC 3.1.1.34 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    7.8 pg/mL-500 pg/mL
  • 靈敏度:
    1.95 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Metabolism
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat LPL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:200Average %93
    Range %87-96
    1:400Average %102
    Range %97-105
    1:800Average %97
    Range %92-101
    1:1600Average %98
    Range %93-101
  • 回收率:
    The recovery of rat LPL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8884-91
    EDTA plasma (n=4)9994-102
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.538 2.512 2.525 2.413
    2502.014 1.977 1.996 1.884
    1251.493 1.464 1.479 1.367
    62.50.801 0.817 0.809 0.697
    31.20.483 0.466 0.475 0.363
    15.60.281 0.272 0.277 0.165
    7.80.194 0.188 0.191 0.079
    00.113 0.111 0.112
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage. Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity. Mediates margination of triglyceride-rich lipoprotein particles in capillaries. Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans.
  • 基因功能參考文獻:
    1. The results of the present study indicated that maternal undernutrition during pregnancy may lead to an increase in hepatic triglycerides, via alterations in the transcriptional and epigenetic regulation of the LPL gene. PMID: 27035287
    2. Cardiomyocyte VEGF activates EC Notch signaling, facilitating GPIHBP1-mediated translocation of LPL across EC and regulating LPL-derived fatty acid delivery to the cardiomyocytes. PMID: 26586663
    3. the amount of LPL expressed in muscle and heart governed both the binding of chylomicron particles and the assimilation of chylomicron lipids in the tissue. PMID: 25589507
    4. Data indicate that oxidation of oleic acid was reduced by 50% in lipoprotein lipase LPL-knock-down (LPL-KD) cells compared to wild-type (WT) cells. PMID: 25931001
    5. Activated cyclin-dependent kinase 5 promotes microglial phagocytosis of fibrillar beta-amyloid by up-regulating lipoprotein lipase expression PMID: 23816988
    6. Suggest that the heparanase-lipoprotein lipase-VEGF axis amplifies fatty acid delivery, a rapid and adaptive mechanism that is geared to overcome the loss of glucose consumption by the diabetic heart. PMID: 24115032
    7. The plasticity of the LPL system is severely blunted or completely lost in insulin resistant rats. PMID: 23176178
    8. active heparanase released lipoprotein lipase from the myocyte surface PMID: 23471235
    9. Data suggest that endothelial lipoprotein lipase (LPL) levels in the heart are altered to maintain FA flux and may exploit angiopoietin-like protein-4 (ANGPTL4). PMID: 22226882
    10. Report a simple procedure for measuring lipoprotein lipase activity. PMID: 22870821
    11. In acute hyperglycemia and moderate diabetes, exaggerated LPL processing to dimeric, catalytically active enzyme increases coronary LPL, delivering more FA to the heart when glucose utilization is compromised. PMID: 21646389
    12. This study showed that adiponectin mediates actin cytoskeleton remodeling to translocate lipoprotein lipase and allow subsequent activation. PMID: 21147877
    13. When compared with obesity-resistant rats, the gene expression of LPL was higher in obesity-prone rats. PMID: 17712951
    14. The over-expression of LPL in the hippocampus may play an important role in the oxidative stress mechanisms following status epilepticus (SE) by regulating the uptake of vitamin E. PMID: 20497648
    15. Down-regulation of adipose tissue lipoprotein lipase during fasting requires that a gene, separate from the lipase gene, is switched on PMID: 11809775
    16. An HFS diet induces changes in LPL and VLDL-R in a manner which favors shunting of dietary fat from skeletal muscle to adipose tissue. PMID: 11882325
    17. characterized changes in LPL activity in adipose tissue and muscles before and during the early phase of obesity development in the JCR:LA-cp rat PMID: 11896485
    18. STZ-induced diabetes causes a decrease in LPL activity in the sciatic nerve that is reversible with insulin treatment PMID: 11897675
    19. insulin deficiency impairs the expression of LPL in mammary glands, revealing the role of insulin as a modulator of the enzyme at the mRNA expression level PMID: 11900280
    20. The hormonal responses of LPL activity and lipolysis in adipose tissue differed depending on the stage of the estrous cycle. PMID: 12032743
    21. Although Lpl increases arterial wall accumulation of triglyceride-rich particles(TGRPs), it also reduces the penetration of TGRPs into the arterial wall--a novel antiatherogenic property. PMID: 12482838
    22. PPAR-gamma activation mediates adipose depot-specific effects on gene expression and activity of this enzyme. PMID: 12540599
    23. the nutritional regulation of LPL in adipose tissue determines the activity state of extracellular LPL. PMID: 12551943
    24. the striking sensitivity of muscle lipoprotein lipase to inactivity and low-intensity contractile activity may indicate why inactivity is a risk factor for metabolic diseases PMID: 12815182
    25. Rapid "metabolic switching" occurs through Lpl occupation of endothelial binding-sites. PMID: 12967632
    26. AMPK-mediated recruitment of LPL to the coronary lumen could represent an immediate compensatory response by the heart to guarantee fatty acid supply. PMID: 15328075
    27. The decrease of LPL mass and activity that occurs when explants of rat adipose tissue are incubated is due to proteolysis of extracellular LPL. The effect of inhibitors indicates, but does not prove, that the degradation is mediated by MMPs. PMID: 15670333
    28. Lysophosphatidic acid induced increases in cardiac lipoprotein lipase occurred via posttranscriptional mechanisms and processes. PMID: 15681706
    29. Upregulated by rosiglitazone PMID: 15887043
    30. Chronic stress decreased LPL in mesenteric and epididymal white adipose tissue(WAT). Acute stress decreased LPL only in retroperitoneal WAT and increased LPL in preheparin plasma active LPL. PMID: 15947029
    31. The finding that nephrotic serum alters lipoprotein lipase (LpL) binding to rat aortic endothelial cells suggests LpL depletion results from decreased binding rather than defective delivery. PMID: 16807550
    32. Angiopoietin-like protein 4 is a fasting-induced controller of lipoprotein lipase in adipose tissue, acting extracellularly on the native conformation in an unusual fashion, like an unfolding molecular chaperone PMID: 17088546
    33. Accelerated triglyceride clearance was associated with increased Lpl activity, mostly in brown adipose tissue. PMID: 17170230
    34. the release from endothelium to the bloodstream may constitute a fast nonexplored mechanism of tissue LPL activity regulation, involved in the lipid energy-substrate redistribution between tissues needed to prepare the "fight-or-flight" respo PMID: 17259660
    35. hormonal and nutritional induction of liver PPAR-alpha expression around birth and its maintained elevated level throughout suckling is responsible for the induction of liver LPL-expression and activity during suckling PMID: 17451160
    36. Suggest the involvement of brain phosphatidylinositol-specific PLC and diacylglycerol lipase in the centrally administered histamine-induced activation of the adrenomedullary outflow in rats. PMID: 17628524
    37. oxidized fat impairs normal metabolic adaptations during lactation, which promote the utilization of metabolic substrates by the mammary gland for the synthesis of milk PMID: 17709442
    38. Administration of resistin to adipocytes mimicked the effects of GIP on the PKB/LKB1/AMPK/LPL pathway: increasing phosphorylation of PKB, reducing levels of phosphorylated LKB1 and AMPK, and increasing LPL activity. PMID: 17890220
    39. Recruitment of LPL to the cardiomyocyte surface represents an immediate compensatory response by the heart to guarantee fatty acid supply when glucose utilization is compromised. PMID: 17942824
    40. after diabetes, Protein kinase D control of LPL requires dissociation of Hsp25 from PKCdelta, association between PKCdelta and PKD, and vesicle fission. PMID: 18583709
    41. Cold acclimation significantly increased heparin-releasable LPL (P<0.05) and tissue residual LPL (P<0.01)in the heart. AMPK phosphorylation does not mediate the enhanced translocation of LPL to cardiac endothelium. PMID: 18722549
    42. Epididymal white adipose tissue perfused in situ with an nitric oxide donor rapidly releases LPL from the endothelium. PMID: 19088434

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  • 亞細胞定位:
    Cell membrane; Peripheral membrane protein; Extracellular side. Secreted. Secreted, extracellular space, extracellular matrix.
  • 蛋白家族:
    AB hydrolase superfamily, Lipase family
  • 數據庫鏈接:

    KEGG: rno:24539

    STRING: 10116.ENSRNOP00000016543

    UniGene: Rn.3834



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