在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Rat inducible nitric oxide synthase,iNOS ELISA KIT

  • 中文名稱:
    大鼠誘導型一氧化氮合成酶(iNOS)酶聯免疫試劑盒
  • 貨號:
    CSB-E08325r
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    大鼠誘導型一氧化氮合成酶(iNOS)酶聯免疫試劑盒(CSB-E08325r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的NOS2含量。NOS2即誘導型一氧化氮合酶,在炎癥等病理狀態下可被誘導表達。其背景在于在免疫防御等方面發揮重要作用。研究機制上,它能催化生成大量一氧化氮,參與調控炎癥反應、細胞凋亡等過程,和多種疾病的發生發展關聯緊密。試劑盒檢測范圍為0.78 IU/mL-50 IU/mL,為研究相關疾病模型的分子機制提供了可靠工具。檢測體系覆蓋0.78至50 IU/mL的線性范圍,適用于評估實驗動物模型中iNOS的動態變化,如炎癥因子誘導的組織損傷研究、藥物干預效果分析或免疫調節機制探索。通過檢測iNOS的表達差異,可深入解析其在細胞信號通路中的作用,為心血管疾病、神經退行性病變等領域的機制研究提供數據支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Nos2 ELISA Kit; Nitric oxide synthase ELISA Kit; inducible ELISA Kit; EC 1.14.13.39 ELISA Kit; Inducible NO synthase ELISA Kit; Inducible NOS ELISA Kit; iNOS ELISA Kit; NOS type II ELISA Kit; Peptidyl-cysteine S-nitrosylase NOS2 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.78 IU/mL-50 IU/mL
  • 靈敏度:
    0.195 IU/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat iNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %96
    Range %90-100
    1:2Average %94
    Range %89-99
    1:4Average %100
    Range %95-104
    1:8Average %102
    Range %98-105
  • 回收率:
    The recovery of rat iNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9189-95
    EDTA plasma (n=4)9389-97
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    IU/ml. OD1OD2AverageCorrected
    502.209 2.267 2.238 2.103
    251.504 1.534 1.519 1.384
    12.50.954 0.961 0.958 0.823
    6.250.598 0.575 0.587 0.452
    3.120.348 0.369 0.359 0.224
    1.560.254 0.267 0.261 0.126
    0.780.201 0.211 0.206 0.071
    00.134 0.136 0.135  
  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能:
    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8.
  • 基因功能參考文獻:
    1. Early changes in impedance in response to I/R is related to histopathological changes, the nuclear stabilization and translocation of HIF-1alpha as well as expression of iNOS. PMID: 29451295
    2. A modulatory influence of the iNOS on the functional activity of the KATP-channels in coronary vessels of rats adapted to the stress has been reported. PMID: 30193054
    3. The results suggest that GPR30 activation inhibits myocardial fibrosis in diabetic ovariectomy female rats by suppressing cardiac iNOS activity and consequently nitric oxide levels. PMID: 30227089
    4. Study in surgery induced uremia rats suggest that chronic kidney disease could induce intestinal inflammation and lead to intestinal dysmotility, which may be associated with iNOS activation in the intestine. PMID: 29393230
    5. Gla treatment downregulated inducible nitric oxide synthase (iNOS) gene expression. PMID: 28707183
    6. Elevated TNF-alpha, nitric oxide (NO) and cytokines-IL-6 and IL-1beta with upregulated NF-kappaB/TNF-alpha/iNOS signalling pathways were effectively down-regulated by tangeretin. PMID: 28871498
    7. Expression of iNOS mRNA in the ischemic myocardial region increased significantly in the process of myocardial ischemic reperfusion injury. PMID: 29308569
    8. By down-regulation of common peripheral and local NFkappaB-iNOS pathway. PMID: 28009008
    9. findings suggest that TNF-alpha decreases mesenteric lymphatic contractility by activating the NF-kappaB-iNOS signaling pathway. PMID: 28231612
    10. In the dorsal raphe nucleus, iNOS plays a role in the anxiety-like behavior induced by ethanol withdrawal. PMID: 27139232
    11. AMPK activation upregulates cytokine-stimulated hepatocyte iNOS expression. PMID: 29229115
    12. HIgh fat diet increases testicular iNOS expression and this is not altered by antioxidants. PMID: 29023053
    13. Central MAPKs/PI3K/sGC pathways variably contribute to the TNF-alpha/iNOS-dependent reductions in blood pressure and heart rate variability seen during endotoxic shock. PMID: 27110744
    14. unpredictable chronic mild stress decreases penile constitutive eNOS and iNOS expression via TNF-alpha, which may contribute to the development of erectile dysfunction PMID: 27904147
    15. Our findings suggest that treatment with EPO or IPC and their combination improve renal function and preserve tubular damage induced by IR injury. These advantageous effects were closely related to reducing oxidative stress, suppressing proinflammatory response and enhancing generation of NO. IPC was more powerful in enhancement of eNOS gene expression compared to EPO that was more effective in increasing inos. PMID: 28471344
    16. Dopamin neurons exposed to preformed alpha-synuclein fibrils displayed a marked increase in iNOS protein levels compared to vehicle-treated neurons PMID: 28534083
    17. The findings suggest that shear stress upregulated AMPK phosphorylation in vascular smooth muscle cells via NOS expression may be a beneficial route to prevent pathogenesis in the vascular system. PMID: 28694192
    18. Both NOS I and NOS II are present in the thoracic spinal cord and are tonically active under physiological conditions. NO derived from NOS II exerts a tonic inhibitory action on sympathetic outflow from the sympathetic preganglionic neurons that targets primarily the blood vessels. PMID: 27371683
    19. Tetrandrine prevents monocrotaline-induced pulmonary arterial hypertension in rats through regulation of the iNOS and PKG-1 signaling pathway. PMID: 26527422
    20. An animal model of chronic psychosocial stress, protected against hyper-production of iNOS in different brain structures. PMID: 27649096
    21. Treatment with etaftorone decreased expression of inducible NOS and XOR in kidneys, whereas it increased the expression of endothelial NOS. PMID: 26663724
    22. R59949, a diacylglycerol kinase inhibitor, inhibits iNOS nitric oxide production by decreasing transplasmalemmal L-arginine uptake in vascular smooth muscle cells. PMID: 27909743
    23. The results thus demonstrate that XO mediates oxidative stress in MB+PQ-treated rat PMNs via iNOS-independent but cytokine (predominantly IFN-gamma)-dependent mechanism. PMID: 28025796
    24. Folic acid/vitamin B12 may be possible nutritional intervention against nicotine-induced pancreatic islet cell damage by inhibiting inos and the generation of inflammation mediators. PMID: 27832994
    25. Immunosuppressive agents reduced expression of INOS/ENOS by coronary vessels to prevent transplant vasculopathy of cardiac allografts. PMID: 26924260
    26. Data suggest iNos plays role in pathogenesis (i.e., effects on other organs) of acute kidney injury, here due to ischemia/reperfusion or unilateral nephrectomy; specifically, iNos appears to play role in loss of function that occurs in contralateral kidney (but does not occur in lungs). PMID: 26854976
    27. injection of 10 mug/kg/12h progesterone hormone reduced the TNF-alpha and iNOS gene expression significantly and confirmed the role of progesterone in the reduction of inflammation. PMID: 27306339
    28. Review: inhibition of iNOS expression may be useful in preventing drug-induced liver injury. PMID: 27026409
    29. iNOS, but not prostaglandin E2, may play a primary role in delayed functional closure of the Ductus Arteriosus. PMID: 26821579
    30. The neonatal malnutrition impaired the animals' body weight. There was a lower expression of the inducible nitric oxide enzyme (iNOS), nitric oxide production, and viability of macrophages infected with methicillin-resistant Staphylococcus aureus. PMID: 25701093
    31. Antioxidant diet in both genders is associated with less PAS-positive staining and less mesangial expansion than the regular diet, an early increase in cortical iNOS in males, and sex-specific changes in cortical eNOS at 20 weeks. PMID: 26797190
    32. These findings indicated that honokiol protects against renal ischemia/reperfusion injury via the suppression of oxidative stress, iNOS, inflammation and STAT3 in the rat. PMID: 26647858
    33. Overactivation of the adenosine system may foster iNOS-mediated diabetic vascular dysfunction. PMID: 26879172
    34. Combined stress increased iNOS expression, primarily in the CA3 area of the ventral hippocampus. PMID: 26608376
    35. Results from this study indicate that exogenous ghrelin induces expression and activity of cardiac iNOS via Akt phosphorylation followed by NFkappaB activation in HF rats. PMID: 26600052
    36. Up-regulation of y(+)LAT2 transporter is coupled with increased inducible nitric oxide synthase (iNOS) expression PMID: 26448619
    37. Data show that curcumin can recover learning and memory function through down-regulating hippocampal tumor necrosis factor alpha (TNF-alpha) and inducible nitric oxide synthase (iNOS) levels. PMID: 26927554
    38. Cromoglycate ameliorated the injurious effect of warm ischemia/reperfusion in rat liver by inhibiting iNOS activity. PMID: 26396497
    39. The hearts from hypothyroid rats were resistant to Ischemia-reperfusion injury, partly due to the lower expression of iNOS and subsequent reduction in apoptosis after Ischemia-reperfusion. PMID: 26774797
    40. CAPE (Caffeic Acid Phenethyl Ester) affects expression of iNOS and cystathionine gamma-lyase in the hepatic fibrosis rat model PMID: 26378818
    41. While myocardial TLR4, MPO and ADMA levels increased in the MIR group, these parameters were found to be decreased significantly in the group treated with apocynin. PMID: 25404055
    42. HIF-1alpha may regulate the expression of iNOS and be involved in the damage of intestinal mucosa in hypobaric hypoxia. PMID: 26191216
    43. Inhibition of NOS-Nitric oxide system prevents autoimmune orchitis development in rats. PMID: 26046347
    44. Study showed that the mRNA expression of iNOS significantly increased in the group of experimental spinal cord injury, as compared with the sham-operated and the healthy control groups PMID: 25644387
    45. Data suggest that, in polycystic ovary syndrome, heart exhibits up-regulation of iNOS (nitric oxide synthase 2) activity, but down-regulation of both eNOS (nitric oxide synthase 3) and Na+/K+-ATPase (Na+/K+ transporting ATPase alpha 1) activities. PMID: 25988879
    46. Vitamin K1 attenuates NF-kappaB and iNOS expression in rat pancreas. PMID: 25466668
    47. Results indicate that inducible nitric oxide synthase (iNOS) expression within the ventrolateral medulla is differentially regulated by iNOS blockade that may, in part, contribute to the modulation of cardiovascular responses during static exercise. PMID: 25234194
    48. Results show that iNOS expression is dispensable to the course of tri-nitrobenzene sulphonic acid colitis, but is crucial in causing neural damage at early time points PMID: 25562655
    49. Our results demonstrated that ventricular hypertrophy abrogated isoflurane-induced delayed cardioprotection by alteration of iNOS/COX-2 pathway PMID: 25400168
    50. Profound cerebral artery vasodilation after traumatic brain injury is due to a gain of function in vascular NO production by 60-fold over controls, by iNOS induction in the endothelium. PMID: 25527626

    顯示更多

    收起更多

  • 亞細胞定位:
    Cytoplasm, cytosol.
  • 蛋白家族:
    NOS family
  • 組織特異性:
    In normal kidney, expressed primarily in the medullary thick ascending limb, with minor amounts in the medullary collecting duct and vasa recta bundle.
  • 數據庫鏈接:


主站蜘蛛池模板: 国产又色又爽又黄刺激的视频| 少妇人妻丰满做爰xxx| 搡老熟女国产| 欧美黑人疯狂性受xxxxx喷水| 又污又爽又黄的网站| 久久精品亚洲精品国产色婷| 四虎永久在线精品免费无码 | 免费午夜男女高清视频| 偷偷色噜狠狠狠狠的777米奇| 久久高清内射无套| 日本精品αv中文字幕| 毛片大全真人在线| 少妇性荡欲午夜性开放视频剧场| 国产精品无码久久av| 免费播放一区二区三区| 成人片无码免费播放| 亚洲欧美不卡高清在线| 日本免费大黄在线观看| 午夜片神马影院福利| 日韩精品无码免费一区二区三区 | 久久精品麻豆日日躁夜夜躁| 西西人体44www大胆无码| 国产精品久久无码不卡黑寡妇| 久久99亚洲精品久久69| 亚洲综合色无码| 无码国产色欲xxxx视频| 波多野结衣av在线无码中文18 | 国产 中文 制服丝袜 另类| 特级精品毛片免费观看| 1000部啪啪未满十八勿入下载| 亚洲国产成人精品无码区在线软件| 成年片色大黄全免费网站久久高潮| 亚洲人成网站色www| 国产精品无码mv在线观看 | 天堂www中文资源| 无码人妻精品一区二区蜜桃色欲| 欧美野外疯狂做受xxxx高潮| 国产办公室无码视频在线观看| 久久精品国产亚洲αv忘忧草| 99re久久精品国产| 久99久热只有精品国产女同|