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Rat corticotropin releasing hormone,CRH ELISA Kit

  • 中文名稱:
    大鼠促腎上皮質激素釋放激素(CRH)酶聯免疫試劑盒
  • 貨號:
    CSB-E08038r
  • 規格:
    96T/48T
  • 價格:
    ¥3900/¥2500
  • 其他:

產品詳情

  • 產品描述:
    大鼠促腎上皮質激素釋放激素(CRH)酶聯免疫試劑盒(CSB-E08038r)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的CRH含量。CRH(促腎上腺皮質素釋放激素)是一種肽激素,參與應激反應,由CRH基因編碼。它主要刺激垂體合成促腎上腺皮質激素。CRH在慢性疼痛和抑郁的神經環路中發揮重要作用,其調控機制涉及神經環路和突觸傳遞,為相關疾病的治療提供了新的靶點。試劑盒檢測范圍為1.25 ng/mL-20 ng/mL,該產品適用于科研領域中對大鼠應激模型、神經內分泌疾病機制的研究,以及通過檢測外周或中樞組織中的 CRH 水平探究其生理病理調控機制。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Crh ELISA Kit; Corticoliberin ELISA Kit; Corticotropin-releasing factor ELISA Kit; CRF ELISA Kit; Corticotropin-releasing hormone ELISA Kit
  • 縮寫:
    CRH
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    1.25 ng/mL-20 ng/mL
  • 靈敏度:
    1.25 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 89-97  
    1:2 Average % 95  
    Range % 89-99  
    1:4 Average % 86  
    Range % 82-93  
    1:8 Average % 101  
    Range % 97-105  
  • 回收率:
    The recovery of rat CRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 90-96  
    EDTA plasma (n=4) 90 85-96  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    20 0.244 0.237 0.241    
    10 0.509 0.525 0.517    
    5 0.854 0.907 0.881    
    2.5 1.757 1.742 1.750    
    1.25 2.571 2.437 2.504    
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Hormone regulating the release of corticotropin from pituitary gland. Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile.
  • 基因功能參考文獻:
    1. These findings robustly demonstrate aberrant interactions of stress and reward networks after early-life adversity and suggest mechanistic roles for Crh-expressing amygdala neurons in emotional deficits portending major neuropsychiatric disorders. PMID: 29033027
    2. CRF (25-200 nM) concentration-dependently diminished evoked compound excitatory postsynaptic potentials, but increased miniature excitatory postsynaptic current frequencies similarly in central amygdala neurons of both naive and ethanol-dependent rats, indicating reduced evoked glutamatergic responses and enhanced vesicular glutamate release, respectively. CRF primarily acts at presynaptic CRF1. PMID: 28807676
    3. the results from the present study suggest that the activation of the CRF system in anxiety-related regions (such as the cingulate cortex, the hippocampus, the amygdala) PMID: 28935440
    4. Results of the present study indicate that corticotropin-releasing factor neurons are the output neurons of the oval nucleus of the bed nucleus of the stria terminalis and they send projections not only to the centres of neuroendocrine and autonomic regulation, but also regions modulating reward and motivation, vigilance and motor function, as well as affective behaviour. PMID: 27805752
    5. Chronic restraint stress (5 weeks, 3h/day) attenuated CRF expression in the paraventricular nucleus of the hypothalamus and dentate gyrus of high-anxiety rats, which was probably due to dysregulation of hormonal feedback mechanisms and enhancement of local neurodegenerative processes; induced symptoms of anhedonia (decreased consumption of 1% sucrose solution) in high-anxiety rats. PMID: 27150225
    6. Results from this study demonstrate the convergence of three important systems, norepinephrine, corticotropin-releasing factor, and delta opioid receptors, in the amygdala and provide insight into their functional role in modulating stress and anxiety responses. PMID: 27376372
    7. The CRF plays important roles in responses to stress in extrahypothalamic circuits such as the mesocorticolimbic dopamine system. PMID: 26387568
    8. QRFP-dependent pathways are involved in the regulation of CRF gene expression in the hypothalamus. PMID: 27452579
    9. These results suggest that predator odor and EPM exposure activates CRF neurons in the BNST to a much greater extent than CRF neurons of the central amygdala. PMID: 26821289
    10. Corticotropin-releasing hormone was increased in the paraventricular nucleus following chronic mild stress. PMID: 26578428
    11. This study provides evidence that corticotropin releasing factor neurons in the central nucleus of the amygdala may play a role in the anxiety-like state produced in a subset of rats exposed to footshocks. PMID: 26363852
    12. Chronic noise stress upregulated CRH mRNA levels in the hypothalamus. PMID: 26333123
    13. immunofluorescence double staining showed that CRF was extensively colocalized with neurons, but hardly with astrocytes or microglia PMID: 26138585
    14. Neuronal TLR4/MCP-1 signal was sustained during alcohol drinking by increased expression of corticotropin-releasing factor and its feedback regulation of TLR4 expression, likely contributing to the transition to alcohol dependence PMID: 25567426
    15. Electroacupuncture significantly reduces visceral hypersensitivity in rats, and regulated the expression of CRH protein and mRNA in the colon, spinal cord and hypothalamus. PMID: 26109804
    16. Study shows that pain-induced anxiety is mediated by corticotropin-releasing factor neurotransmission in the locus coeruleus through extracellular signal-regulated kinases 1/2 signaling cascade PMID: 25716783
    17. Prenatal stress is associated with the demethylation of the CRH promoter. PMID: 24682755
    18. The differentiated behavioral stress responses were reflected by gene expression changes in the pituitary. Alterations in the mRNA levels of Crh, Ucn2 and Ucn3 in the pituitary might confirm the paracrine and/or autocrine effects of these peptides PMID: 25236411
    19. A significantly increased hippocampal CRH was observed in the adult rats with postnatal maternal separation. PMID: 24718660
    20. Results show that hypertension triggers downregulation of CRF gene expression in the amygdala and significantly alters the heart rate response to acute stress but does not alter the pressor response to stress compared to normotensive controls PMID: 25139171
    21. differential effects of RLN3 on CRF expression in the paraventricular hypothalamic nucleus and bed nucleus of the stria terminalis may contribute to the sex-specific difference in behavioral response PMID: 25406021
    22. In this mini-review, we will focus on novel and evolving concepts regarding the potential mechanisms underlying the short and long-term effects of prenatal stress involving CRH peptide family. PMID: 25433848
    23. The expression profile of this key limbic brain CRF system might contribute to the complex neural mechanisms underlying the increasing incidence of early onset of puberty on the one hand and infertility on the other attributed to chronic stress. PMID: 25051447
    24. Overexpression of FosB or cJun potently increases CRF mRNA levels. PMID: 24246425
    25. Transcriptional repression of CRH by glucocorticoids involves protein-protein interactions and/or modulation of afferent inputs to the hypothalamic paraventricular nucleus. PMID: 24065704
    26. We can conclude that the orexigenic effect of mu-opioid receptor activation by EM-2 could be related to both inhibition of CRH and stimulation of dopamine and norepinephrine levels, in the hypothalamus PMID: 23916912
    27. demonstrated the existence of genetically determined high basal CRF mRNA levels in central amygdala of Sardinian alcohol-preferring rats. PMID: 24021806
    28. data demonstrate stable changes in methylation patterns and expression of the GR and CRF genes following repeated stress. PMID: 23084728
    29. Alcohol self-administration reduces the number of CRF-labeled cells in the central amygdala. PMID: 23628776
    30. data suggest that the observed impairment in learning was not a result of alteration in HPA axis activity, but rather due to reduced PVN-CRF activity. PMID: 23568325
    31. studies demonstrate that CRF neurons within the lateral BNST modulate conditioned anxiety-like behaviors and also suggest that enhanced CRF expression within these neurons may contribute to inappropriate regulation of emotional memories PMID: 22290119
    32. Studied levels of amygdaloid CRF in the anxiolytic effect of acupuncture during ethanol withdrawal. PMID: 24139460
    33. Over-expression of CRH and vasopressin in the magnocellular paraventricular nucleus represents a specific feature of anxiety/post traumatic stress disorder-like state. PMID: 24317347
    34. The imbalance in lower production of anorexigenic neuropeptide CRF in the paraventricular hypothalamic nucleus may favor overeating and increased body weight gain in chronically stressed food-restricted female rats. PMID: 23425370
    35. ERK1/2 activation in response to CRH is biphasic, involving a first cAMP- and B-Raf-dependent early phase and a second phase that critically depends on CRHR1 internalization and beta-arrestin2. PMID: 23371389
    36. CRF and hypothalamic neuronal histamine mediate the suppressive effects of BDNF on feeding behavior and body weight. PMID: 23432085
    37. CRF acts in the ventral tegmental area to reduce the motivation to work for food rewards by regulating dopamine output in a stimulus- and pathway-specific manner. PMID: 23416448
    38. Interactions between corticosterone and catecholaminergic projections to the hypothalamus therefore make significant contributions to the regulation of Crh and Avp expression. PMID: 22831701
    39. Under conditions associated with CRF release at the locus coeruleus projection system, including stress, the transfer of afferent information within sensory systems is impaired. PMID: 22510725
    40. Corticotropin releasing hormone act on CRHR1 to induce MKLP1 expression via the PLC/PKC signaling pathway. PMID: 22698524
    41. GLP-1 directly stimulated the promoter activities and mRNA levels of both CRF and AVP in hypothalamic 4B cells PMID: 22801106
    42. These data demonstrate that CRF triggers increases in intestinal paracellular permeability via mast cell dependent release of TNF-alpha and proteases. PMID: 22768175
    43. Hypomethylation of the CRH promoter CRE, a region critical for CRH transcriptional activation, could serve as a mechanism for the increased transcriptional responses to stress observed in maternally deprived rats. PMID: 22375940
    44. the CRF system may contribute to both the behavioral response during social stress and its behavioral and autonomic consequences PMID: 22322324
    45. Data suggest that CRH regulates dendritic outgrowth in cultured hippocampal neurons/pyramidal cells; signaling via CRH-receptor 1 (CRH-R1) stimulates dendritic growth; CRH- receptor 2 (CRH-R2) activation results in inhibition of dendritic growth. PMID: 22249942
    46. nucleus accumbens shellCRF can generate a variety of aversive behaviors PMID: 22245501
    47. after chronic constriction injury of the rat sciatic nerve, the oval bed nucleus of the stria terminalis contained substantially more Crf mRNA as did the central amygdala PMID: 21684787
    48. These findings indicate that reproductive experience modulates the effects of estrogen receptor alpha activation on both maze behavior related to anxiety and corticotrophin-releasing hormone gene expression. PMID: 22033279
    49. During acute stress, arginine vasopressin interacts with the paraventricular hypothalamic nucleus and amygdala to change their rates of biosynthesis and/or release of corticotrophin releasing factor (CRF). PMID: 21621194
    50. An 8-week ethanol vapos regimen significantly increased CRF levels in the hippocampus and parietal cortex. PMID: 21527271

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  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Sauvagine/corticotropin-releasing factor/urotensin I family
  • 組織特異性:
    Produced by the hypothalamus.
  • 數據庫鏈接:


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