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  4. Rat collagen type Ⅱ(Col Ⅱ) antibody ELISA kit

Rat collagen type Ⅱ(Col Ⅱ) antibody ELISA kit

  • 中文名稱：
    大鼠Ⅱ型膠原(Col Ⅱ)抗體酶聯免疫試劑盒
  • 貨號：
    CSB-EQ027975RA
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠Ⅱ型膠原(Col Ⅱ)抗體酶聯免疫試劑盒（CSB-EQ027975RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的Col Ⅱ Ab含量。試劑盒檢測范圍為0.312 U/mL-20 U/mL，適用于科研領域對骨關節疾病模型的機制研究；適用于評估關節炎動物模型中Col Ⅱ抗體的表達水平，探究軟骨退行性病變的分子機制，或用于篩選調節軟骨代謝的候選化合物；為軟骨生物學、病理學及藥物開發研究提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    collagen type 2(Col 2) antibody,_x000D_ collagen type II(Col II) antibody
  • 縮寫：
    Col Ⅱ Ab
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    0.312 U/mL-20 U/mL
  • 靈敏度：
    0.078 U/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅱ antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 89    
    Range % 86-99    
    1:2 Average % 95    
    Range % 91-100    
    1:4 Average % 93    
    Range % 85-100    
    1:8 Average % 93    
    Range % 82-98    
  • 回收率：
    The recovery of rat Col Ⅱ antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 96 91-110    
    EDTA plasma (n=4) 98 89-100    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    U/ml OD1 OD2 Average Corrected    
    20 2.607 2.725 2.666 2.498    
    10 2.036 2.081 2.059 1.891    
    5 1.328 1.369 1.349 1.181    
    2.5 0.811 0.825 0.818 0.650    
    1.25 0.436 0.421 0.429 0.261    
    0.625 0.349 0.360 0.355 0.187    
    0.312 0.243 0.232 0.238 0.070    
    0 0.169 0.167 0.168 　    
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

相關問答

■ 常見問題解答
Q:

I have to use the CSB-EQ027975RA Rat collagen Type II (Col II) antibody kit and I have some questions:
I have to use Culture Medium not Serum or Plasma is this possible?
In other KIt we use before the Standard (CSB-EO8092r) has a concentration of 1000 pg/mL
now this new kit we are going to use has a concentration of 20 U/mL so whic is the relation between Unit and pg?. How can we calculate Collagen II concentration
Thanks in advance for your help

A:
Thanks for your inquiry! CSB-E08092r is for quantitative determination of collagen type Ⅱ(Col Ⅱ) .
CSB-EQ027975RA is for quantitative determination of collagen type Ⅱ(Col Ⅱ) antibody.
U/mL is a quantitative unit and the standard in this kit is represented by U/mL. Sorry there is no converting relationship with pg/mL.
This kit is usually used to test serum and plasma samples. Sorry we didn't test culture medium before and we can not esure 100% if it can be detected out. We suggest you do a pretest first if you want to test it.


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