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  4. Rat coagulation factor Ⅹ,FⅩ ELISA Kit

Rat coagulation factor Ⅹ,FⅩ ELISA Kit

  • 中文名稱：
    大鼠凝血因子Ⅹ(FⅩ)酶聯免疫試劑盒
  • 貨號：
    CSB-E08441r
  • 規格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠凝血因子Ⅹ(FⅩ)酶聯免疫試劑盒（CSB-E08441r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的F10含量。F10即凝血因子Ⅹ，在凝血級聯反應中至關重要。其可被內、外源凝血途徑激活為F10a，F10a與FVa等結合形成凝血酶原酶復合物，促使凝血酶原轉化為凝血酶，最終使纖維蛋白原變成纖維蛋白，形成血栓，眾多抗凝藥以其為靶點展開研究。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，適用于凝血機制基礎研究、抗凝藥物開發評估以及血栓性疾病動物模型中凝血功能變化的動態監測，例如深靜脈血栓、彌散性血管內凝血等病理過程的機制探索，也可用于肝功能障礙或維生素K缺乏相關模型中凝血因子合成能力的研究本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    F10Coagulation factor X ELISA Kit; EC 3.4.21.6 ELISA Kit; Stuart factor) [Cleaved into: Factor X light chain; Factor X heavy chain; Activated factor Xa heavy chain] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    1.56 ng/mL-100 ng/mL
  • 靈敏度：
    0.39 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Blood Coagulation
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat FⅩ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1000

    Average %

    97

    Range %

    95-102

    1:2000

    Average %

    98

    Range %

    91-105

    1:4000

    Average %

    106

    Range %

    102-109

    1:8000

    Average %

    92

    Range %

    86-98

  • 回收率：

    The recovery of rat FⅩ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    89-98

    EDTA plasma (n=4)

    96

    92-100

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    100

    1.959

    1.914

    1.936

    1.785

    50

    1.679

    1.657

    1.668

    1.517

    25

    1.300

    1.338

    1.319

    1.168

    12.5

    0.993

    0.969

    0.981

    0.830

    6.25

    0.678

    0.651

    0.664

    0.513

    3.12

    0.457

    0.443

    0.450

    0.299

    1.56

    0.377

    0.384

    0.380

    0.229

    0

    0.148

    0.155

    0.151

    　

  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
  • 基因功能參考文獻：
    1. Factor Xa inhibitor edoxaban at or higher doses than needed for an antithrombotic effect sustained the circulating Gla-osteocalcin level. PMID: 22999414
    2. Report differential effects of TAK-442, a novel orally active direct factor Xa inhibitor, and ximelagatran, a thrombin inhibitor, on factor V-mediated feedback on coagulation cascade and bleeding. PMID: 20664909
    3. Factor Xa has a role in inducing mitogenesis of vascular smooth muscle cells by autocrine production of epiregulin PMID: 14570897
    4. The changes in levels of blood coagulation factor X in folic acid deficient rats are reversed by treatment with folic acid. PMID: 16046705
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family
  • 組織特異性：
    Plasma; synthesized in the liver.
  • 數據庫鏈接：


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