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  4. Rat cartilage oligomeric matrix protein,COMP ELISA Kit

Rat cartilage oligomeric matrix protein,COMP ELISA Kit

  • 中文名稱：
    大鼠軟骨寡聚基質蛋白(COMP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13833r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠軟骨寡聚基質蛋白(COMP)酶聯(lián)免疫試劑盒（CSB-E13833r）檢測血清、血漿、組織勻漿樣本中的COMP含量。軟骨寡聚基質蛋白（COMP）是一種細胞外基質蛋白，主要存在于軟骨中，對軟骨細胞分化和基質形成起重要作用。研究發(fā)現(xiàn)，COMP在顳下頜關節(jié)滑膜軟骨瘤病（TMJSC）中表達顯著增高，可能與TGF-β3、TGF-β1、BMP-2等細胞因子協(xié)同作用，促進滑膜細胞軟骨化生及軟骨增殖，從而在TMJSC發(fā)病中起一定作用。試劑盒檢測范圍為78 pg/ml- 5000 pg/ml，靈敏度為19.5 pg/ml。本試劑盒可為骨關節(jié)疾病機制研究、軟骨損傷修復模型評估、骨質疏松相關藥物開發(fā)等科研領域提供定量分析工具，通過檢測體液或組織中的COMP含量變化，助力研究者深入探索軟骨代謝調控網(wǎng)絡及病理生理學機制。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    CompCartilage oligomeric matrix protein ELISA kit; COMP ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    78 pg/ml - 5000 pg/ml
  • 靈敏度：
    19.5 pg/ml
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat COMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    92

    Range %

    86-107

    1:2

    Average %

    97

    Range %

    91-105

    1:4

    Average %

    98

    Range %

    92-104

    1:8

    Average %

    100

    Range %

    95-105

  • 回收率：

    The recovery of rat COMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    96

    91-101

    EDTA plasma (n=4)

    93

    90-96

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    5000

    2.639

    2.699

    2.669

    2.501

    2500

    2.151

    2.163

    2.157

    1.989

    1250

    1.505

    1.542

    1.524

    1.356

    625

    1.062

    1.098

    1.080

    0.912

    312

    0.637

    0.689

    0.663

    0.495

    156

    0.392

    0.399

    0.396

    0.228

    78

    0.332

    0.347

    0.340

    0.172

    0

    0.168

    0.167

    0.168

    　

  • 本試劑盒所含材料：
    • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat COMP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled COMP antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
    • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
    • One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

靶點詳情

  • 功能：
    May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7.
  • 基因功能參考文獻：
    1. COMP could normally have a protective role against PASMC phenotype switching PMID: 28860005
    2. these findings revealed the essential role of COMP in retarding the development of vascular aging and vascular smooth muscle cell senescence. PMID: 27498005
    3. COMP-Ang1-stimulated proliferation, differentiation, and migration of progenitor cells may involve the Tie-2-mediated activation of p38 MAPK and PI3K/Akt pathways PMID: 25446117
    4. results suggest the potential importance of COMP-Ang1 as a new therapy for impaired bone formation that is associated with diabetes and advanced age PMID: 23850469
    5. The presence of cleaved apoptosis inducing factor (tAIF) and the absence of activated caspases indicated that retention of D469del-COMP triggers cell death in chondrocytes by necroptosis, a caspase-independent programmed necrosis. PMID: 22154936
    6. Cartilage oligomeric matrix protein is a novel inhibitor of vascular smooth muscle calcification. PMID: 21350216
    7. Overexpression of COMP markedly suppressed PDGF-BB-elicited VSMC dedifferentiation. Alpha(7)beta(1) integrin is a potential receptor for COMP. PMID: 20019333
    8. Matrix-matrix interaction of cartilage oligomeric matrix protein and fibronectin. PMID: 12225811
    9. The X-ray structure of the COMP coiled coil domain in complex with vitamin D(3) at 1.7 A resolution is reported. COMP exerts a distinct mechanism of interaction mainly defined by the pattern of hydrophobic core residues. PMID: 12426368
    10. COMP has a role in development of the extracellular matrix and in a cell culture model for pseudoachondroplasia PMID: 15579310
    11. COMP secretion is affected by the signal peptide PMID: 15749701
    12. Expression of COMP in both anulus fibrosus(AF) and nucleus pulposus(NP) of intervertebral disc(IVD). COMP is component of extracellular matrix of AF and NP, with lamellar distribution in AF. May play role in normal structure of IVD. PMID: 16778685
    13. ultrastructural distribution of COMP & TSP-4 & collagen fibril thickness in achilles tendon related to exercise; COMP immunolabeling decreased with longer running distance & was lower in pericellular & interterritorial compartments of high training rats PMID: 17882701
    14. To identify residues critical for the structure, stability, oligomerization and binding to vitamin D as well as two small molecules, all-trans-retinol and curcumin, an alanine-scanning mutagenesis study of cartilage oligomeric matrix protein is performed. PMID: 19681593

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  • 亞細胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Thrombospondin family
  • 組織特異性：
    Expressed in differentiated and de-differentiated vascular smooth muscle cells (VSMCs) (at protein level).
  • 數(shù)據(jù)庫鏈接：


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