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  4. Rat calcitonin gene related peptide,CGRP ELISA Kit

Rat calcitonin gene related peptide,CGRP ELISA Kit

  • 中文名稱：
    大鼠降鈣素基因相關肽(CGRP)酶聯免疫試劑盒
  • 貨號：
    CSB-E08211r
  • 規格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠降鈣素基因相關肽(CGRP)酶聯免疫試劑盒（CSB-E08211r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CGRP含量。降鈣素基因相關肽（CGRP）是一種神經肽。背景上，它在疼痛傳導等方面起著重要作用，尤其與偏頭痛發病機制關聯密切。研究機制上，主要聚焦于其受體及信號通路，通過阻斷CGRP及其受體，有望開發出治療偏頭痛等疼痛性疾病的藥物。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于科研場景中CGRP的定量分析，例如神經生物學研究中的痛覺傳導機制探索、心血管疾病模型中的血管舒縮功能評估，或藥理學實驗中對CGRP調控類化合物作用的體外驗證，為相關疾病機制研究及藥物開發評估提供可靠數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Calcb ELISA Kit; Calcitonin gene-related peptide 2 ELISA Kit; Beta-type CGRP ELISA Kit; Calcitonin gene-related peptide II ELISA Kit; CGRP-II ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %80-95
    1:2Average %94
    Range %87-101
    1:4Average %90
    Range %86-106
    1:8Average %95
    Range %82-100
  • 回收率：
    The recovery of rat CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-99
    EDTA plasma (n=4)9488-101
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.742 2.725 2.734 2.562
    2002.137 2.183 2.160 1.988
    1001.384 1.481 1.433 1.261
    500.863 0.806 0.835 0.663
    250.452 0.475 0.464 0.292
    12.50.298 0.292 0.295 0.123
    6.250.203 0.214 0.209 0.037
    00.171 0.173 0.172 　
  • 本試劑盒所含材料：
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Rat CGRP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled CGRP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

相關問答

■ 常見問題解答
Q:

Pls inform me the sequence of the standard in this kit

A:
Thanks for your inquiry. This kit is designed against full length protein.Pls refer to https://www.uniprot.org/uniprot/P10093.The standard in it is 293 cell-derived recombinant protein.The sequence of the standard is confidential.Sorry we can not disclose it. Hope you could understand.

靶點詳情

  • 功能：
    CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.
  • 基因功能參考文獻：
    1. results indicate that calcitonin gene-related peptide-B receptors are present in rat placenta and that receptor levels are higher with gestational age and lower at term labor (Calcb) PMID: 12297551
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Calcitonin family
  • 數據庫鏈接：


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