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中文名稱:大鼠β膠原交聯(bCTx)酶聯免疫試劑盒
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貨號:CSB-EQ027521RA
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:大鼠β膠原交聯(bCTx)酶聯免疫試劑盒(CSB-EQ027521RA)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的bCTx含量。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL,在骨質疏松機制研究、骨病模型評價及骨代謝相關藥物研發中具有重要科研價值;廣泛應用于骨代謝動態監測、抗骨吸收藥物療效評估、骨折愈合研究及骨腫瘤模型構建等科研領域本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:bCTx
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:7.8 pg/mL-500 pg/mL
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靈敏度:1.95 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Others
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat bCTx in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 88-96 1:2 Average % 96 Range % 93-99 1:4 Average % 102 Range % 98-106 1:8 Average % 87 Range % 84-90 -
回收率:
The recovery of rat bCTx spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 87-95 EDTA plasma (n=4) 101 97-106 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 500 2.793 2.878 2.836 2.634 250 1.953 1.830 1.892 1.690 125 1.153 1.215 1.184 0.982 62.5 0.745 0.761 0.753 0.551 31.2 0.532 0.545 0.539 0.337 15.6 0.436 0.405 0.421 0.219 7.8 0.314 0.301 0.308 0.106 0 0.205 0.199 0.202 -
數據處理:
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貨期:3-5 working days
引用文獻
- Metabolomics and physiological analysis of the effect of calcium supplements on reducing bone loss in ovariectomized rats by increasing estradiol levels H Mao,Nutrition & metabolism,2021
- The Impact of Strontium Ranelate on Metaphyseal Bone Healing in Ovariectomized Rats Komrakova M. et al,Calcif Tissue Int.,2015
- Neutrophil serine proteases and their endogenous inhibitors in coronary artery ectasia patients Ruifeng Liu et al,Anatol J Cardiol,2015
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