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  4. Rat apolipoprotein A1 (Apo-A1) ELISA Kit

Rat apolipoprotein A1 (Apo-A1) ELISA Kit

  • 中文名稱：
    大鼠載脂蛋白A1(Apo-A1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08105r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠載脂蛋白A1(Apo-A1)酶聯(lián)免疫試劑盒（CSB-E08105r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APOA1含量。APOA1是重要靶點。它是高密度脂蛋白（HDL）的主要載脂蛋白，與膽固醇逆轉(zhuǎn)運相關(guān)，有助于將外周組織膽固醇轉(zhuǎn)運回肝臟代謝。研究發(fā)現(xiàn)其在動脈粥樣硬化等疾病進程中發(fā)揮關(guān)鍵作用，通過提高其水平或活性有望改善脂質(zhì)代謝、防治相關(guān)疾病。試劑盒檢測范圍為0.14 ng/ml-100 ng/ml.，靈敏度為0.14 ng/ml.。本試劑盒適用于基礎(chǔ)科研中對大鼠模型脂代謝機制的研究，例如通過檢測不同實驗條件下（如基因干預(yù)、飲食調(diào)控或藥物處理）血清或組織中的Apo - A1濃度變化，評估心血管疾病模型的病理進程或干預(yù)效果。為探索Apo - A1在脂質(zhì)穩(wěn)態(tài)及代謝性疾病中的功能提供高效研究工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Apoa1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I)] ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.14 ng/ml-100 ng/ml.
  • 靈敏度：
    0.14 ng/ml.
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1000

    Average %

    94

    Range %

    88-108

    1:2000

    Average %

    97

    Range %

    91-105

    1:4000

    Average %

    103

    Range %

    98-108

    1:8000

    Average %

    93

    Range %

    86-98

  • 回收率：

    The recovery of rat Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    89-102

    EDTA plasma (n=4)

    89

    82-96

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    100

    2.340

    2.434

    2.387

    2.275

    33.33

    1.892

    1.867

    1.880

    1.768

    11.11

    1.157

    1.128

    1.142

    1.031

    3.70

    0.625

    0.639

    0.632

    0.521

    1.23

    0.297

    0.311

    0.304

    0.192

    0.41

    0.188

    0.193

    0.190

    0.079

    0.14

    0.134

    0.140

    0.137

    0.025

    0

    0.108

    0.115

    0.112

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

相關(guān)問答

■ 常見問題解答
Q:


My samples are hemolyzed....could it be any problem with this samples when using the kits?

A:
Thanks for your inquiry. Serious hemolysis is not suitable for testing. You need to re-collect the samples.
If it is slightly hemolyzed, the impact is small and the samples can be used.
The sensitivity of this kit is relatively good, but we still advice you to use dual-wavelength detection to eliminate the impact.

靶點詳情

  • 最新研究進展：
    APOA1是一種高密度脂蛋白(HDL)的主要成分，參與調(diào)節(jié)膽固醇代謝和清除血管內(nèi)的膽固醇。最新研究表明，APOA1可以抑制乳腺癌的惡性轉(zhuǎn)化和肺癌細胞的增殖，可能成為腫瘤治療的新靶點。此外，APOA1水平與糖尿病、心血管疾病和肝病等多種疾病相關(guān)。
  • 功能：
    Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • 基因功能參考文獻：
    1. both the route of administration and the formulation of apoA-I peptide significantly affect its pharmacokinetics and pharmacodynamics. PMID: 27881716
    2. ABCA1 is diminished and ABCG1 is increased by N-acetylcysteine, which counteracts the reduction in apo A-I-mediated cholesterol efflux PMID: 25305669
    3. Apolipoprotein A-I induces tubulin phosphorylation in association with cholesterol release in fetal rat astrocytes. PMID: 24814947
    4. ApoA-I may be a protective blood-borne factor involved in the remote ischemic preconditioning mechanism. PMID: 24155931
    5. It maintains cholesterol homeostasis. PMID: 24117066
    6. on neonatal rat ventricular cardiomyocytes, the aldosterone-dependent chronotropic effects of antiapoA-1 IgG involve the nongenomic activation of L-type calcium channels PMID: 22253414
    7. translocation of cholesterol and phospholipid into the cytosol is related to the apo A-I-mediated HDL assembly in astrocytes through functional association with caveolin-1 and a cyclosporin A-sensitive cyclophilin protein(s). PMID: 11773045
    8. ABCA1-dependent cholesterol mobilization to apoA-I increased new cholesterol synthesis, indicating depletion of the regulatory pool of hepatocyte cholesterol during HDL formation PMID: 14993246
    9. The association of CLPP with microtubules is mediated by caveolin-1, induced by apoA-I and regulates intracellular cholesterol trafficking for assembly of cellular lipids to apoA-I-HDL. PMID: 16606364
    10. Data show that a complex of apolipoprotein A-I and pregnenolone modulate the rate of protein biosynthesis in liver cells. PMID: 18457017
    11. ApoA-I reduced I/R-induced inflammatory responses, decreased renal microscopic damage and improved renal function. ApoA-I protects kidney from I/R injury by inhibiting inflammatory cytokines release and neutrophil infiltration and activation. PMID: 18535924

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Apolipoprotein A1/A4/E family
  • 組織特異性：
    Major protein of plasma HDL, also found in chylomicrons.
  • 數(shù)據(jù)庫鏈接：


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