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中文名稱:大鼠血管活性腸肽(VIP)酶聯免疫試劑盒
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貨號:CSB-E08355r
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:大鼠血管活性腸肽(VIP)酶聯免疫試劑盒(CSB-E08355r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的VIP含量。VIP即血管活性腸肽,具有廣泛生物學活性。它參與調節心血管、消化等系統功能。在研究機制上,VIP通過與特定受體結合,激活細胞內信號通路,影響多種生理和病理過程,在神經保護、抗炎等方面展現潛在治療價值。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL,適用于神經生物學、消化系統疾病模型研究,可分析大鼠外周循環或組織局部VIP表達水平,為探究胃腸動力障礙、炎癥調節機制及神經 - 免疫交互作用提供數據支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:
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Uniprot No.:
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:1.56 pg/mL-100 pg/mL
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靈敏度:0.39 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Neuroscience
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat VIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 94 Range % 90-98 1:2 Average % 97 Range % 93-101 1:4 Average % 100 Range % 97-103 1:8 Average % 86 Range % 82-92 -
回收率:
The recovery of rat VIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 90-98 EDTA plasma (n=4) 90 86-94 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 100 2.384 2.506 2.445 2.324 50 1.732 1.813 1.773 1.652 25 1.112 1.100 1.106 0.985 12.5 0.615 0.626 0.621 0.500 6.25 0.386 0.397 0.392 0.271 3.12 0.297 0.286 0.292 0.171 1.56 0.196 0.193 0.195 0.074 0 0.121 0.120 0.121 -
數據處理:
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貨期:3-5 working days
引用文獻
- Proteomic Insights into the Effects of Jianweixiaoshi Tablets on Functional Dyspepsia with Spleen Deficiency in Rats X Cheng, J Wan, D Sun, Y Zhan, J Yu, Y Li,Drug Design, Development and Therapy,2024
- Chai-Qin-Cheng-Qi Decoction and Carbachol Improve Intestinal Motility by Regulating Protein Kinase C-Mediated Ca2+ Release in Colonic Smooth Muscle Cells in Rats with Acute Necrotising Pancreatitis. Zhang CL.et al,Evid Based Complement Alternat Med. ,2017
- Granulocyte colony-stimulating factor provides protection against cardiovascular autonomic neuropathy in streptozotocin-induced diabetes in rats Erba? O.et al,Diabetes Res Clin Pract.,2015
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