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Rat Undercarboxylated Osteocalcin (ucOC) ELISA Kit

  • 中文名稱:
    大鼠羧化不全骨鈣素(ucOC)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E17798r
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠羧化不全骨鈣素(ucOC)酶聯(lián)免疫試劑盒(CSB-E17798r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的ucOC含量。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL,適用于多種科研場景,如評估骨組織工程材料的成骨效果、探究藥物或營養(yǎng)干預(yù)對骨代謝的影響、構(gòu)建骨相關(guān)疾病動物模型后的生物標(biāo)志物分析等。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    ucOC
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.156 ng/mL-10 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat ucOC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 97
    Range % 87-101
    1:2 Average % 101
    Range % 94-105
    1:4 Average % 95
    Range % 88-98
    1:8 Average % 102
    Range % 94-107
  • 回收率:
    The recovery of rat ucOC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 83-94
    EDTA plasma (n=4) 100 94-105
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.642 2.567 2.605 2.498
    5 2.157 2.102 2.130 2.023
    2.5 1.424 1.376 1.400 1.293
    1.25 0.706 0.729 0.718 0.611
    0.625 0.398 0.385 0.392 0.285
    0.312 0.247 0.241 0.244 0.137
    0.156 0.163 0.156 0.160 0.053
    0 0.105 0.109 0.107  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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