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  4. Rat Tumor necrosis factor β,TNF-β ELISA KIT

Rat Tumor necrosis factor β,TNF-β ELISA KIT

  • 中文名稱：
    大鼠腫瘤壞死因子β(TNF-β)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07379r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠腫瘤壞死因子β(TNF-β)酶聯(lián)免疫試劑盒（CSB-E07379r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TNF-β含量。TNF-β，又稱淋巴毒素-α，是腫瘤壞死因子超家族成員，由淋巴細(xì)胞產(chǎn)生。其激活NF-κB信號通路，誘導(dǎo)癌細(xì)胞增殖、侵襲，并介導(dǎo)多種炎癥、免疫和抗病毒反應(yīng)。研究其機(jī)制有助于開發(fā)針對腫瘤、炎癥等疾病的藥物。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，尤其適用于動物模型研究中TNF-β的動態(tài)監(jiān)測，例如炎癥性疾病建模、免疫調(diào)節(jié)實(shí)驗(yàn)或抗腫瘤藥物作用評估等場景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Lta ELISA Kit; Tnfb ELISA Kit; Tnfsf1 ELISA Kit; Lymphotoxin-alpha ELISA Kit; LT-alpha ELISA Kit; TNF-beta ELISA Kit; Tumor necrosis factor ligand superfamily member 1 ELISA Kit
  • 縮寫：
    TNF-β
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 101
    Range % 96-105
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 87
    Range % 83-94
    1:8 Average % 96
    Range % 91-102
  • 回收率：
    The recovery of rat TNF-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 104 99108
    EDTA plasma (n=4) 88 84-93
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    500 2.632 2.765 2.699 2.614
    250 2.212 2.302 2.257 2.172
    125 1.545 1.577 1.561 1.476
    62.5 0.765 0.771 0.768 0.683
    31.2 0.411 0.423 0.417 0.332
    15.6 0.257 0.264 0.261 0.176
    7.8 0.176 0.185 0.181 0.096
    0 0.084 0.085 0.085 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.
  • 基因功能參考文獻(xiàn)：
    1. Adrenomedullin can increase the expression of TNF-beta and TNFR in renal early trauma. PMID: 20092780
    2. TNFbeta-so far only known to be produced by lymphocytes-was demonstrated in alveolar macrophages, bronchial epithelial cells, vascular smooth muscle cells and endothelial cells at the protein and the message level PMID: 12849708
  • 亞細(xì)胞定位：
    Secreted. Membrane.
  • 蛋白家族：
    Tumor necrosis factor family
  • 數(shù)據(jù)庫鏈接：


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