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  4. Rat Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ ELISA KIT

Rat Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ ELISA KIT

  • 中文名稱：
    大鼠腫瘤壞死因子可溶性受體Ⅱ(TNFsR-Ⅱ)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07380r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠腫瘤壞死因子可溶性受體Ⅱ(TNFsR-Ⅱ)酶聯(lián)免疫試劑盒（CSB-E07380r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TNFsR2含量。TNFsR2 是重要靶點(diǎn)。TNF 家族參與多種生理病理過程，TNFsR2 作為其受體之一，在免疫調(diào)節(jié)、細(xì)胞存活與凋亡等方面發(fā)揮作用。研究聚焦其與配體結(jié)合機(jī)制，以及在疾病如炎癥、腫瘤中的信號(hào)傳導(dǎo)通路，旨在為相關(guān)疾病治療提供新思路。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于科研實(shí)驗(yàn)中動(dòng)態(tài)監(jiān)測動(dòng)物模型（如關(guān)節(jié)炎、膿毒癥等）的炎癥進(jìn)程、評(píng)估藥物干預(yù)對受體表達(dá)水平的影響，或探究TNF信號(hào)通路相關(guān)疾病的分子機(jī)制。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Tnfrsf1b ELISA Kit; Tnfr2 ELISA Kit; Tumor necrosis factor receptor superfamily member 1B ELISA Kit; Tumor necrosis factor receptor 2 ELISA Kit; TNF-R2 ELISA Kit; Tumor necrosis factor receptor type II ELISA Kit; TNF-RII ELISA Kit; TNFR-II ELISA Kit; p75 ELISA Kit; p80 TNF-alpha receptor ELISA Kit; CD antigen CD120b ELISA Kit
  • 縮寫：
    TNFsR-
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TNFsR-II in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 91
    Range % 87-94
    1:2 Average % 97
    Range % 92-101
    1:4 Average % 90
    Range % 87-98
    1:8 Average % 92
    Range % 85-95
  • 回收率：
    The recovery of rat TNFsR-II spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 93 88-96
    EDTA plasma (n=4) 98 95-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    40 2.827 2.899 2.863 2.732
    20 2.222 2.323 2.273 2.142
    10 1.373 1.412 1.393 1.262
    5 0.816 0.866 0.841 0.710
    2.5 0.531 0.545 0.538 0.407
    1.25 0.315 0.323 0.319 0.188
    0.625 0.226 0.241 0.234 0.103
    0 0.126 0.135 0.131 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2.
  • 基因功能參考文獻(xiàn)：
    1. results suggested that PTE may promote BMSC proliferation and osteogenic differentiation via a mechanism associated with the regulation of Let-7f-5p and the TNFR2/PI3K/AKT signaling pathway. PMID: 29975930
    2. Curcumin decreased TNFR2 and increased TRAF2 to decrease the apoptotic pathway in ureteral obstruction rats. PMID: 26732833
    3. The IL-17RD.TNFR2 complex in activation of NF-kappaB may explain the role of TNFR2 in inflammatory diseases including nephritis. PMID: 25378394
    4. the microglial TNF-alpha p55 pathway played a more important role than the TNF-alpha p75 pathway in the pathogenesis of peripheral nerve injury pain. PMID: 22652595
    5. study of TNF-alpha and TnfrR2 protein synthesis and expression patterns in sciatic nerve of controls and rats under systemic challenge with lipopolysaccharide. PMID: 17873366
    6. In human biopsies and in rat kidney allografts on day 6 with acute allograft rejection, significantly elevated expression of TNFR2 was observed. PMID: 19298452

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  • 亞細(xì)胞定位：
    Membrane; Single-pass type I membrane protein.
  • 數(shù)據(jù)庫鏈接：


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