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  4. Rat Thyroglobulin,TG ELISA Kit

Rat Thyroglobulin,TG ELISA Kit

  • 中文名稱：
    大鼠甲狀腺球蛋白(TG)酶聯免疫試劑盒
  • 貨號：
    CSB-E08240r
  • 規格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠甲狀腺球蛋白(TG)酶聯免疫試劑盒（CSB-E08240r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TG含量。大鼠甲狀腺球蛋白（TG）是甲狀腺濾泡細胞合成的糖蛋白，參與甲狀腺激素的合成和儲存。其負反饋調節機制涉及抑制甲狀腺激素合成基因表達，與促甲狀腺激素（TSH）共同調控甲狀腺功能，對甲狀腺癌的診斷和預后評估具有重要意義。試劑盒檢測范圍為10 ng/mL-160 ng/mL，適用于科研領域對甲狀腺功能及相關疾病的機制研究；適用于甲狀腺相關藥物的作用評估、環境毒素對甲狀腺影響的機制研究，以及甲狀腺疾病動物模型中病理進程的動態監測本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    TgThyroglobulin ELISA kit; Tg ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue Homogenates
  • 檢測范圍：
    10 ng/mL-160 ng/mL
  • 靈敏度：
    10 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 95
    Range % 89-102
    1:2 Average % 92
    Range % 86-99
    1:4 Average % 95
    Range % 87-105
    1:8 Average % 90
    Range % 83-102
  • 回收率：
    The recovery of rat TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 89-100
    EDTA plasma (n=4) 95 88-103
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average
    10 2.553 2.568 2.561
    20 1.748 1.763 1.756
    40 0.885 0.875 0.880
    80 0.515 0.511 0.513
    160 0.285 0.287 0.286
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling. Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream. One dimer produces 7 thyroid hormone molecules.
  • 相關疾病：
    Defects in Tg are a cause of a form of hypothyroidism in rdw rat.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Type-B carboxylesterase/lipase family
  • 組織特異性：
    Specifically expressed in the thyroid gland.
  • 數據庫鏈接：