在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Rat Testosterone,T ELISA Kit

  • 中文名稱:
    大鼠睪酮(T)酶聯免疫試劑盒
  • 貨號:
    CSB-E05100r
  • 規格:
    96T/48T
  • 價格:
    ¥3900/¥2500
  • 其他:

產品詳情

  • 產品描述:
    大鼠睪酮(T)酶聯免疫試劑盒(CSB-E05100r)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的T含量。大鼠睪酮(T)是男性激素,對生殖功能和男性特征發育至關重要。其合成主要在睪丸間質細胞(Leydig細胞)中進行,受促性腺激素(LH)調控。睪酮水平異常與多種生殖和生理問題相關,如少弱精子癥、性功能障礙等。研究睪酮的作用機制有助于開發治療相關疾病的藥物和方法。試劑盒檢測范圍為0.13 ng/mL-25.6 ng/mL,靈敏度為0.06 ng/mL。動物實驗研究中的激素水平監測,如生殖生物學機制探索、藥物或環境因素對內分泌系統的影響評估、以及疾病模型(如代謝綜合征或性腺功能障礙)的睪酮動態變化分析等領域本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    N/A
  • 縮寫:
    T
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍:
    0.13 ng/mL-25.6 ng/mL
  • 靈敏度:
    0.06 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat testosteronen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-96  
    1:2 Average % 97  
    Range % 92-104  
    1:4 Average % 85  
    Range % 81-88  
    1:8 Average % 89  
    Range % 83-93  
  • 回收率:
    The recovery of rat testosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 87-95  
    EDTA plasma (n=4) 94 90-98  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    25.6 0.238 0.236 0.237    
    6.4 0.435 0.431 0.433    
    2 0.611 0.615 0.613    
    0.5 0.984 0.982 0.983    
    0.13 1.403 1.398 1.401    
    0 2.001 2.003 2.002    
  • 本試劑盒所含材料:
      • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit Testosterone antibody.
      • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • Anti-Testosterone Antibody 1 x 6 ml -- Act as the detection antibody.
      • HRP-conjugated Testosterone 1 x 6 ml -- Bind to the DHT antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
      • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
      • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
      • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
      • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

    顯示更多

    收起更多

  • 本試劑盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

    顯示更多

    收起更多

  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 最新研究進展:
    T4,全稱為甲狀腺素(thyroxine),是由甲狀腺合成和分泌的一種甲狀腺激素。T4在人體內具有重要的生理作用,它能夠促進蛋白質、脂肪和碳水化合物的代謝,并參與調節心血管、神經、消化、泌尿系統等多種生理功能。近年來,T4的研究熱點主要集中在其與疾病的關聯性,如甲狀腺功能減退癥、自身免疫性甲狀腺疾病、心血管疾病等。此外,T4也被廣泛應用于臨床上,如用于甲狀腺激素替代治療和輔助診斷甲狀腺相關疾病等。


主站蜘蛛池模板: 大陆国语对白国产av片| 撕开奶罩揉吃奶高潮av在线观看| 97免费人妻在线视频| 国产播放隔着超薄丝袜进入| 青春草在线视频观看| 西西人体44www大胆无码| 国产成人一区二区三区在线观看| 亚洲国产超清无码专区| 加勒比色综合久久久久久久久| 苍井空浴缸大战猛男120分钟| 国产sm主人调教女m视频| 夜夜欢性恔免费视频| 久久99亚洲精品久久久久| 久久丫精品系列| 国产激情久久久久久熟女老人av| 久久97国产超碰青草| 久九九精品免费视频| 亚洲一区二区三区四区| 理论片87福利理论电影| 精品毛片乱码1区2区3区| 成人无码a∨电影免费| 天美传媒一区二区| 色婷婷久久综合中文久久一本| 国产毛片不卡野外视频| 成人亚洲a片v一区二区三区动漫| 韩日午夜在线资源一区二区| 欧美 日韩 亚洲 在线| 亚洲国产成人久久一区久久| 中文字幕亚洲欧美在线不卡| 国产成人一区二区三区别| 香蕉久久av一区二区三区app| 无码熟妇人妻av在线网站| 窝窝午夜看片| 婷婷四房综合激情五月| 亚洲va天堂va欧美ⅴa在线 | 久久99九九精品久久久久蜜桃| 欧美成人猛片aaaaaaa| 波多野结衣av在线无码中文18| 成在人线av无码免费看网站直播| 男女啪动最猛动态图| 日本熟妇色xxxxx日本免费看|