Rat Tenascin-C(TN-C) ELISA Kit
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中文名稱:大鼠粘膠蛋白/肌腱蛋白C(TN-C)酶聯免疫試劑盒
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貨號:CSB-E13377r
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:大鼠粘膠蛋白/肌腱蛋白C(TN-C)酶聯免疫試劑盒(CSB-E13377r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的TN-C含量。TN - C是當前靶點。它在細胞外基質中發揮重要作用,與細胞黏附、遷移等過程相關。研究其機制聚焦于它和腫瘤微環境、腫瘤細胞生長及轉移的關系,通過揭示TN - C作用機制或為腫瘤等相關疾病的治療提供新靶點與思路。試劑盒檢測范圍為2.5 ng/mL-160 ng/mL,科研人員可將其應用于心血管疾病模型、肝臟纖維化研究或創傷修復機制探索,通過檢測不同病理狀態下TN - C的動態表達,揭示其在細胞黏附、信號轉導中的功能,為靶向治療策略提供基礎數據支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:TN-C
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:2.5 ng/mL-160 ng/mL
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靈敏度:0.625 ng/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Neuroscience
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat TN-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 88-96 1:2 Average % 84 Range % 80-88 1:4 Average % 106 Range % 101-113 1:8 Average % 93 Range % 89-98 -
回收率:
The recovery of rat TN-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 90-100 EDTA plasma (n=4) 88 84-92 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 160 2.417 2.502 2.460 2.301 80 2.102 2.173 2.138 1.979 40 1.700 1.615 1.658 1.499 20 1.085 1.049 1.067 0.908 10 0.671 0.684 0.678 0.519 5 0.424 0.437 0.431 0.272 2.5 0.293 0.281 0.287 0.128 0 0.162 0.156 0.159 -
數據處理:
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貨期:3-5 working days
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