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Rat Tau Proteins ELISA kit

  • 中文名稱:
    大鼠Tau蛋白酶聯免疫試劑盒
  • 貨號:
    CSB-E13729r
  • 規格:
    96T/48T
  • 價格:
    ¥3900/¥2500
  • 其他:

產品詳情

  • 產品描述:
    大鼠Tau蛋白酶聯免疫試劑盒(CSB-E13729r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、腦脊液(CSF)樣本中的MAPT含量。MAPT基因編碼tau蛋白,是神經退行性疾病如額顳葉癡呆(FTDP-17)、阿爾茨海默病(AD)等的關鍵基因。其突變導致tau蛋白與微管結合能力下降,引發神經纖維纏結,進而導致神經元損傷。研究MAPT基因突變與tau蛋白功能異常的機制,有助于開發針對神經退行性疾病的診斷和治療策略。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL,適用于動態監測實驗動物模型中Tau蛋白的表達變化;適用于神經系統疾病的基礎研究、藥物干預效果評估或病理進程分析;為神經生物學、藥理學及疾病機制探索提供高效工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Mapt ELISA Kit; Mtapt ELISA Kit; Tau ELISA Kit; Microtubule-associated protein tau ELISA Kit; Neurofibrillary tangle protein ELISA Kit; Paired helical filament-tau ELISA Kit; PHF-tau ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, cerebrospinal fluid (CSF)
  • 檢測范圍:
    62.5 pg/mL-4000 pg/mL
  • 靈敏度:
    15.6 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat tau proteins in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %98
    Range %95-101
    1:10Average %90
    Range %88-92
    1:20Average %85
    Range %81-89
    1:40Average %98
    Range %95-101
  • 回收率:
    The recovery of rat tau proteins spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10199-105
    EDTA plasma (n=4)8784-90
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.694 2.678 2.686 2.523
    20002.104 2.156 2.130 1.967
    10001.330 1.356 1.343 1.180
    5000.825 0.836 0.831 0.668
    2500.552 0.568 0.560 0.397
    1250.359 0.368 0.364 0.201
    62.50.276 0.298 0.287 0.124
    00.167 0.159 0.163
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

相關問答

 常見問題解答
Q:

I want to know if CSB-E13729r detects Tau regardless of phosphorylation state?

A:
Thanks for your inquiry.
CSB-E13729r detect the total Tau, including the phosphorylated form.Pls let me know if you have any further questions. Thank you.

靶點詳情

  • 功能:
    Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
  • 亞細胞定位:
    Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell projection, axon. Cell projection, dendrite. Secreted.
  • 組織特異性:
    Expressed in neurons. The larger forms (isoform tau-A and isoform tau-B) are preferentially expressed in the peripheral nervous system while the other are expressed in the central nervous system. Low amounts of the larger forms are also found in limited a
  • 數據庫鏈接:


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