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  4. Rat Syndecan-1/CD138(SDC1) ELISA Kit

Rat Syndecan-1/CD138(SDC1) ELISA Kit

  • 中文名稱：
    大鼠Syndecan-1/CD138(SDC1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E17115r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠Syndecan-1/CD138(SDC1)酶聯(lián)免疫試劑盒（CSB-E17115r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的SDC1含量。SDC1是一個(gè)靶點(diǎn)。它是一種細(xì)胞表面蛋白聚糖，在多種生理和病理過(guò)程中發(fā)揮作用。研究顯示，其參與細(xì)胞增殖、黏附、遷移等機(jī)制。在腫瘤領(lǐng)域，SDC1表達(dá)異常與腫瘤發(fā)生、發(fā)展及轉(zhuǎn)移密切相關(guān)，成為腫瘤治療等方面的研究熱點(diǎn)。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，該產(chǎn)品可廣泛應(yīng)用于大鼠模型研究中SDC1表達(dá)的動(dòng)態(tài)監(jiān)測(cè)，例如探究炎癥性疾病發(fā)展機(jī)制、腫瘤微環(huán)境中細(xì)胞間相互作用，或評(píng)估組織損傷修復(fù)過(guò)程中SDC1的調(diào)控功能，為分子機(jī)理研究及藥物干預(yù)效果評(píng)價(jià)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Sdc1 ELISA Kit; Synd1 ELISA Kit; Syndecan-1 ELISA Kit; SYND1 ELISA Kit; CD antigen CD138 ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SDC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 88-102  
    1:2 Average % 101  
    Range % 95-104  
    1:4 Average % 90  
    Range % 83-94  
    1:8 Average % 94  
    Range % 87-99  
  • 回收率：
    The recovery of rat SDC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 102 94-107  
    EDTA plasma (n=4) 100 91-104  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.458 2.422 2.440 2.264  
    10 2.004 1.976 1.990 1.814  
    5 1.307 1.345 1.326 1.150  
    2.5 0.728 0.737 0.733 0.557  
    1.25 0.432 0.448 0.440 0.264  
    0.625 0.333 0.342 0.338 0.162  
    0.312 0.251 0.243 0.247 0.071  
    0 0.174 0.178 0.176    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.
  • 基因功能參考文獻(xiàn)：
    1. Plasma syndecan-1 and heparan sulfate correlate with microvascular glycocalyx degradation in hemorrhaged rats after different resuscitation fluids. PMID: 27037369
    2. down-regulation of Syndecan-1 could inhibit VEGF-VEGFR-2 signaling through regulating internalization of VEGFR-2. PMID: 27075925
    3. expression upregulated two months after renal transplantation PMID: 25972509
    4. data suggest that after renal transplantation loss of hepatic HS together with increased syndecan-1 shedding hampers lipoprotein binding and uptake by the liver contributing to dyslipidemia PMID: 25154787
    5. analysis of the molecular events that drive endocytosis of syndecan-1, a raft-dependent receptor and identification of its novel endocytic motif, MKKK PMID: 23525115
    6. The abnormal expression of HSPG may be an important molecular mechanism that leads to the occurrence and development of proteinuria in adriamycin-induced nephropathy rats. PMID: 20506637
    7. role in regulatory mechanism of apoptosis PMID: 11979781
    8. Syndecan-1 mRNA expression is not modulated by FSH as it would result from the antagonistic effects of increased intracellular cAMP and intracellular calcium levels. PMID: 12135485
    9. The major injury-related change, seen in injured brain and cultured glia, was an increase in 2-O-sulphated HS and increased syndecan-1, suggesting novel approaches to modulating scar formation. PMID: 18279312

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  • 亞細(xì)胞定位：
    Membrane; Single-pass type I membrane protein. Secreted. Secreted, extracellular exosome.
  • 蛋白家族：
    Syndecan proteoglycan family
  • 數(shù)據(jù)庫(kù)鏈接：


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