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中文名稱:大鼠基質細胞衍生因子1a(SDF-1a/CXCL12)酶聯免疫試劑盒
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貨號:CSB-E08729r
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規格:96T/48T
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價格:¥3900/¥2500
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其他:
產品詳情
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產品描述:大鼠基質細胞衍生因子1a(SDF-1a/CXCL12)酶聯免疫試劑盒(CSB-E08729r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的SDF1a/CXCL12含量。SDF1a/CXCL12是重要的趨化因子。其背景上,在胚胎發育、免疫調節等生理過程發揮關鍵作用。研究機制方面,與受體CXCR4結合,介導細胞遷移、增殖和存活,在腫瘤轉移、炎癥等病理進程中有重要影響,是相關疾病治療潛在靶點。試劑盒檢測范圍為0.78 pg/mL-50 pg/mL,可廣泛應用于神經再生、造血調控、免疫細胞趨化性等基礎研究領域,為探索SDF - 1a/CXCL12在疾病模型中的動態變化提供高特異性檢測工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:SDF1a/CXCL12
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Uniprot No.:
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍:0.78 pg/mL-50 pg/mL
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靈敏度:0.195 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Immunology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat SDF-1a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 88 Range % 83-97 1:200 Average % 97 Range % 91-103 1:400 Average % 84 Range % 80-88 1:800 Average % 89 Range % 81-95 -
回收率:
The recovery of rat SDF-1a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 89-96 EDTA plasma (n=4) 97 94-100 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 50 2.830 2.775 2.803 2.666 25 2.326 2.453 2.390 2.253 12.5 1.600 1.632 1.616 1.479 6.25 0.937 0.894 0.916 0.779 3.12 0.528 0.532 0.530 0.393 1.56 0.321 0.335 0.328 0.191 0.78 0.157 0.148 0.153 0.016 0 0.140 0.133 0.137 -
數據處理:
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貨期:3-5 working days
引用文獻
- CP-25 exerts anti-angiogenic effects on a rat model of adjuvant-induced arthritis by promoting GRK2-induced downregulation of CXCR4-ERK1/2 signaling in endothelial cells Zhang M, et al,Molecular Medicine Reports,2019
- Omega-3 Fatty Acids Supplementation Differentially Modulates the SDF-1/CXCR-4 Cell Homing Axis in Hypertensive and Normotensive Rats Halmenschlager L.et al,Nutrients.,2017
- Auditory stimulation modulates CXCL12/CXCR4 expression in postnatal development of the newborn rat cochlea Zhang W. et al,Neuroreport,2015
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