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  4. Rat Renalase/ Monoamine Oxidase-C(RNLS/ MAO-C)ELISA Kit

Rat Renalase/ Monoamine Oxidase-C(RNLS/ MAO-C)ELISA Kit

  • 中文名稱：
    大鼠腎胺酶(RNLS/ MAO-C)酶聯免疫試劑盒
  • 貨號：
    CSB-EL002965RA
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠腎胺酶(RNLS/ MAO-C)酶聯免疫試劑盒（CSB-EL002965RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C10orf59含量。大鼠腎胺酶（RNLS/MAO-C）是一種黃素蛋白，主要在腎臟表達，能夠降解兒茶酚胺，具有改善心功能、降壓和保護心肌的作用。近年來研究發現，RNLS與糖尿病及其心腦血管并發癥的發生發展密切相關，其基因多態性與糖尿病的遺傳易感性有關，并在糖尿病腎臟疾病、高血壓、腦卒中等并發癥中發揮重要作用。試劑盒檢測范圍為62.5 ng/mL-4000 ng/mL，適用于基礎醫學研究，如探索高血壓、糖尿病腎病等動物模型中腎胺酶的表達調控機制，或用于藥理學研究評估藥物對腎臟氧化應激通路的影響。本產品僅限科學研究使用，不涉及任何臨床診斷用途。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Rnls ELISA Kit; Renalase ELISA Kit; EC 1.6.3.5 ELISA Kit; Monoamine oxidase-C ELISA Kit; MAO-C ELISA Kit; alpha-NAD(P)H oxidase/anomerase ELISA Kit
  • 縮寫：
    C10orf59
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    62.5 ng/mL-4000 ng/mL
  • 靈敏度：
    15.6 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat RNLS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 92
    Range % 88-96
    1:2 Average % 106
    Range % 101-113
    1:4 Average % 94
    Range % 90-98
    1:8 Average % 89
    Range % 84-93
  • 回收率：
    The recovery of rat RNLS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 87 82-91
    EDTA plasma (n=4) 95 90-100
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    4000 2.204 2.132 2.168 1.972
    2000 1.619 1.524 1.572 1.376
    1000 1.002 0.948 0.975 0.779
    500 0.628 0.663 0.646 0.450
    250 0.430 0.407 0.419 0.223
    125 0.295 0.281 0.288 0.092
    62.5 0.241 0.250 0.246 0.050
    0 0.193 0.199 0.196 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Catalyzes the oxidation of the less abundant 1,2-dihydro-beta-NAD(P) and 1,6-dihydro-beta-NAD(P) to form beta-NAD(P)(+). The enzyme hormone is secreted by the kidney, and circulates in blood and modulates cardiac function and systemic blood pressure. Lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis.
  • 基因功能參考文獻：
    1. The brain and peripheral tissues renalase mRNA levels demonstrate opposite trends, which are obviously crucial for impaired regulation of blood pressure in spontaneously hypertensive rats. PMID: 24113803
    2. exercise differentially regulates renalase expression in skeletal muscle and kidney in rats PMID: 24366404
    3. Renalase deficiency occurs in salt-sensitive Dahl rats as they develop hypertension[review] PMID: 17565281
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Renalase family
  • 數據庫鏈接：


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