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  4. Rat Pulmonary Surfactant-associated protein C (SP-C) ELISA kit

Rat Pulmonary Surfactant-associated protein C (SP-C) ELISA kit

  • 中文名稱：
    大鼠肺表面活性物質相關蛋白C(SP-C)酶聯免疫試劑盒
  • 貨號：
    CSB-E12631r
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    大鼠肺表面活性物質相關蛋白C(SP-C)酶聯免疫試劑盒（CSB-E12631r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SFTPC含量。SFTPC 是一個重要靶點。它與肺部表面活性物質相關，在維持肺部正常功能中起關鍵作用。其研究機制聚焦于探究它在肺部疾病發生發展中的具體作用，如在特發性肺纖維化等疾病中的表達變化及作用通路，以尋求潛在治療策略。試劑盒檢測范圍為4.69 pg/mL-300 pg/mL，該產品可應用于基礎研究中評估肺發育、肺泡上皮功能相關機制，或用于肺纖維化、急性呼吸窘迫綜合征（ARDS）等疾病模型中SP - C的動態變化分析，亦可用于評估藥物或外界刺激對肺組織SP - C表達的影響，為呼吸道疾病研究提供可靠的科研工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Sftpc ELISA Kit; Sftp2 ELISA Kit; Pulmonary surfactant-associated protein C ELISA Kit; SP-C ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Val) ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    4.69 pg/mL-300 pg/mL
  • 靈敏度：
    1.17 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1000 Average % 85
    Range % 80-90
    1:2000 Average % 104
    Range % 100-109
    1:4000 Average % 91
    Range % 85-96
    1:8000 Average % 96
    Range % 91-103
  • 回收率：
    The recovery of rat SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 98 94-102
    EDTA plasma (n=4) 87 83-91
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    300 2.402 2.274 2.338 2.227
    150 1.939 2.051 1.995 1.884
    75 1.411 1.497 1.454 1.343
    37.5 0.939 0.904 0.922 0.811
    18.75 0.568 0.579 0.574 0.463
    9.38 0.355 0.382 0.369 0.258
    4.69 0.248 0.260 0.254 0.143
    0 0.114 0.108 0.111 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
  • 基因功能參考文獻：
    1. SP-C is present on the surface of exocytosed surfactant particles. Antibodies against SP-C stabilize the released particles when contacting the air-liquid interface, precluding their transformation and the formation of surface films. SP-C acts as an essential, preformed molecular key in the initial stages of surfactant unpacking and surface film formation. PMID: 27155084
    2. misfolded surfactant protein C has a role in endoplasmic reticulum stress in epithelial-mesenchymal transition of alveolar epithelial cells PMID: 21169555
    3. Results suggest that there is no primary deficiency of surfactant proteins A, B, or C in the nitrofen-induced rat model of congenital diaphragmatic hernia. PMID: 12904592
    4. surfactant protein C processing requires a type II transmembrane topology directed by juxtamembrane positively charged residues PMID: 12933801
    5. NO mediated the decrease in surfactant protein gene expression PMID: 15640287
    6. Data indicate that heparin can be stimulatory to surfactant protein B and C gene expression depending on concentration, degree of sulfation, and surrounding fibroblast growth factor environment. PMID: 16794256

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  • 亞細胞定位：
    Secreted, extracellular space, surface film.
  • 數據庫鏈接：

    KEGG: rno:50683

    STRING: 10116.ENSRNOP00000015032

    UniGene: Rn.3926



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