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  4. Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit

Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit

  • 中文名稱：
    大鼠肺表面活性物質相關蛋白B(SP-B)酶聯免疫試劑盒
  • 貨號：
    CSB-E12630r
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠肺表面活性物質相關蛋白B(SP-B)酶聯免疫試劑盒（CSB-E12630r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SFTPB含量。SP-B是肺表面活性物質的重要組成部分，參與調節肺泡表面張力，維持呼吸功能。其研究機制涉及炎癥反應、氧化應激，與肺發育障礙密切相關。近年來，深度學習等技術在藥物-靶標相互作用預測方面取得進展，為SP-B相關疾病研究提供新思路。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，可廣泛用于基礎科研中肺部疾病機制研究、藥物干預效果評估或動物模型（如肺損傷、肺纖維化及新生兒呼吸窘迫綜合征）的SP - B動態監測，為探索SP - B在呼吸生理及病理過程中的調控作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Sftpb ELISA Kit; Sftp3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
  • 縮寫：
    SFTPB
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.12 pg/mL-200 pg/mL
  • 靈敏度：
    0.78 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1000Average %90
    Range %85-100
    1:2000Average %94
    Range %87-98
    1:4000Average %90
    Range %85-96
    1:8000Average %94
    Range %90-98
  • 回收率：
    The recovery of rat SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9690-101
    EDTA plasma (n=4)9185-97
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.470 2.589 2.530 2.377
    1002.115 2.124 2.120 1.967
    501.713 1.726 1.720 1.567
    251.315 1.356 1.336 1.183
    12.50.985 0.971 0.978 0.825
    6.250.532 0.541 0.537 0.384
    3.120.378 0.389 0.384 0.231
    00.152 0.154 0.153 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
  • 基因功能參考文獻：
    1. SP-B is present on the surface of exocytosed surfactant particles. Antibodies against SP-B stabilize the released particles when contacting the air-liquid interface, precluding their transformation and the formation of surface films. SP-B acts as an essential, preformed molecular key in the initial stages of surfactant unpacking and surface film formation. PMID: 27155084
    2. Liraglutide activates the GLP1 receptor, increasing ACE2 expression, reversing right ventricle hypertrophy, and improving the production of SP-A and SP-B in the lungs of Type 1 Diabetes rats PMID: 26196539
    3. The role of the SP-B NH(2) terminus in the formation and stability of cylindrical structures, specifically the distribution of lipid stack height, width, and density with four SP-B truncation peptides, was studied. PMID: 20023175
    4. VEGF increased expression of SP-b mRNA in preterm rat lung. PMID: 17267143
    5. alterations of surfactant are a consequence of the ventilation strategy that impair the biophysical activity of this material and thereby contribute directly to lung dysfunction over time PMID: 18344412

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  • 亞細胞定位：
    Secreted, extracellular space, surface film.
  • 數據庫鏈接：


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