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  4. Rat Protein S100-A1(S100A1) ELISA kit

Rat Protein S100-A1(S100A1) ELISA kit

  • 中文名稱：
    大鼠S100-A1蛋白(S100A1)酶聯免疫試劑盒
  • 貨號：
    CSB-EL020622RA
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠S100-A1蛋白(S100A1)酶聯免疫試劑盒（CSB-EL020622RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的S100A1含量。S100A1屬于S100蛋白家族成員，主要分布于心肌和骨骼肌等組織。它參與細胞內鈣信號調節，可調控心肌收縮與舒張。研究發現其異常表達與心肌病等疾病密切相關，有望成為治療心血管疾病等的潛在靶點，為相關疾病診治帶來新思路。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，適用于基礎科研中探索S100A1在心血管疾病、肌肉功能調控或代謝性疾病模型中的分子機制，可定量分析不同實驗條件下（如藥物干預、基因編輯或病理刺激）生物樣本中S100A1的動態變化，為相關領域的蛋白功能研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    S100a1 ELISA Kit; Protein S100-A1 ELISA Kit; S-100 protein alpha chain ELISA Kit; S-100 protein subunit alpha ELISA Kit; S100 calcium-binding protein A1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    6.25 ng/mL-400 ng/mL
  • 靈敏度：
    1.56 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat S100A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 93
    Range % 89-97
    1:2 Average % 87
    Range % 82-91
    1:4 Average % 98
    Range % 94-102
    1:8 Average % 101
    Range % 95-107
  • 回收率：
    The recovery of rat S100A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 90-100
    EDTA plasma (n=4) 84 80-88
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    400 2.043 2.001 2.022 1.851
    200 1.295 1.327 1.311 1.140
    100 0.888 0.852 0.870 0.699
    50 0.608 0.640 0.624 0.453
    25 0.430 0.460 0.445 0.274
    12.5 0.346 0.329 0.338 0.167
    6.25 0.255 0.238 0.247 0.076
    0 0.168 0.174 0.171 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation. In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes. These changes allow interactions with specific target proteins and modulate their activity. Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase. Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole.
  • 基因功能參考文獻：
    1. depletion of S100A1 was observed in ischemic cardiomyocytes, and S100A1 plasma concentration was also significantly higher than that of the sham-operated group PMID: 23683996
    2. S100A1 is involved in the maintenance of the genetic program that defines normal myocardial function and its downregulation is permissive for the induction of genes that underlie myocardial hypertrophy PMID: 12619862
    3. S100A1 and calmodulin compete for the same binding site on ryanodine receptor PMID: 18650434
    4. in sympathetic neurons exogenous S100A1 may lead to an increase of sympathetic output. PMID: 19657060
  • 亞細胞定位：
    Cytoplasm. Sarcoplasmic reticulum. Mitochondrion.
  • 蛋白家族：
    S-100 family
  • 組織特異性：
    Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
  • 數據庫鏈接：


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