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  4. Rat Polypeptide YY(PYY)ELISA Kit

Rat Polypeptide YY(PYY)ELISA Kit

  • 中文名稱：
    大鼠多肽YY/酪酪肽(PYY)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13432r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠多肽YY/酪酪肽(PYY)酶聯(lián)免疫試劑盒（CSB-E13432r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的PYY含量。PYY（肽YY）是一種由腸道內(nèi)分泌細(xì)胞產(chǎn)生的激素，主要功能是調(diào)節(jié)食欲和抑制胰腺分泌。近期研究發(fā)現(xiàn)，PYY還具有抗菌肽功能，在維持腸道健康和免疫平衡中發(fā)揮重要作用。其研究機(jī)制涉及PYY與神經(jīng)肽Y受體結(jié)合，調(diào)節(jié)食欲和腸道微生物組，為肥胖、糖尿病等疾病的治療提供了新的靶點。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，適用于科研領(lǐng)域中對大鼠模型能量代謝機(jī)制、胃腸功能調(diào)控或肥胖相關(guān)疾病的研究，例如通過檢測血清或組織中的PYY水平評估飲食干預(yù)、藥物處理或基因修飾對代謝通路的影響，亦可用于體外細(xì)胞實驗探究激素分泌調(diào)控機(jī)制。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    PyyPeptide YY ELISA kit; PYY ELISA kit; Peptide tyrosine tyrosine) [Cleaved into: Peptide YY(3-36 ELISA kit; PYY-II)] ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PYY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %90-100
    1:2Average %88
    Range %84-93
    1:4Average %90
    Range %85-94
    1:8Average %99
    Range %94-105
  • 回收率：
    The recovery of rat PYY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9792-101
    EDTA plasma (n=4)10095-106
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.478 2.374 2.426 2.318
    2501.754 1.852 1.803 1.695
    1251.188 1.226 1.207 1.099
    62.50.725 0.689 0.707 0.599
    31.20.403 0.382 0.393 0.285
    15.60.272 0.257 0.265 0.157
    7.80.189 0.178 0.184 0.076
    00.110 0.105 0.108
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility.
  • 基因功能參考文獻(xiàn)：
    1. Peptide YY mediates the satiety effects of diets enriched with whey protein fractions in male rats PMID: 29042449
    2. Additional analysis of the findings demonstrated significant interactions of ovarian cycle stage and fed/fasted status with age on GLP-1, but not PYY plasma concentrations PMID: 28237410
    3. These findings indicate that the mechanism underlying type 2 diabetes remission may be mediated by PYY. PMID: 27117413
    4. Serum PYY levels were nonsignificantly decreased in rats with human neuroblastoma and hepatoblastoma transplants. PMID: 25111274
    5. gut PYY(3-36) action at Y2 receptors peripheral to the blood brain barrier plays an essential role in mediating satiety PMID: 23482449
    6. RACK1 modulates the ability of preglomerular vascular smooth muscle cells to respond to the proliferative actions of PYY. PMID: 23303411
    7. Exercise in hypoxia suppressed hunger and increased PYY but did not influence ad libitum energy intake. PMID: 22114179
    8. PYY concentrations in plasma, descending colon and rectum tissues were increased (P < 0.001) throughout pregnancy and lactation. PYY peaked at day 5 of lactation in plasma, as well as descending colon and rectum tissues. PMID: 19778563
    9. PYY induced a contraction of smooth muscle strips from proximal colon, but not on smooth muscle from any other gut segments. PMID: 12383864
    10. findings indicate that insulin-like growth factor-I functions as a positive regulator of peptide YY gene expression and that the stimulatory effect may be mediated by Sp1 proteins PMID: 14592960
    11. PYY(3-36) might play a role in the neuroendocrine modulation of the reproductive axis PMID: 15564330
    12. observations indicate that Peptide YY (PYY) levels are influenced by age, thyroid hormones, and growth hormone and indicate that PYY could be involved in the changes of food intake associated with these conditions PMID: 15687395
    13. study indicates that peripheral Peptide YY (PYY) may transmit satiety signals to the brain in part via the vagal afferent pathway PMID: 15718279
    14. PYY-immunoreactive (ir) cell bodies were localized exclusively to the gigantocellular reticular nucleus (Gi) of the rostral medulla. The highest density of PYY fibers was present within the solitary tract nucleus. PMID: 18022952
    15. Demonstrate the development of a potent homeostatic response to increase food intake when PYY(3-36) reduces food intake and energy reserves in diet-induced obese rats. PMID: 18550871

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    NPY family
  • 數(shù)據(jù)庫鏈接：


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