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  4. Rat Plasminogen (Plg) ELISA kit

Rat Plasminogen (Plg) ELISA kit

  • 中文名稱：
    大鼠纖溶酶原(Plg)酶聯免疫試劑盒
  • 貨號：
    CSB-E17250r
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠纖溶酶原(Plg)酶聯免疫試劑盒（CSB-E17250r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Plg含量。Plg 即纖溶酶原，是纖溶系統重要成分。其在體內以無活性前體形式存在，被激活后轉化為纖溶酶，可降解纖維蛋白凝塊。研究聚焦于其激活機制及相關調控因子，對血栓性疾病治療和病理機制探索有重要意義。試劑盒檢測范圍為62.5 ng/mL-4000 ng/mL，靈敏度為15.6 ng/mL。該產品適用于心血管疾病模型研究、創傷修復機制探索或肝臟功能評估等科研領域，為體外實驗提供穩定可靠的數據支持，特別適用于需要批量檢測的實驗室環境。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Plg ELISA Kit; Plasminogen ELISA Kit; EC 3.4.21.7) [Cleaved into: Plasmin heavy chain A; Activation peptide; Angiostatin; Plasmin heavy chain A ELISA Kit; short form; Plasmin light chain B] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    62.5 ng/mL-4000 ng/mL
  • 靈敏度：
    15.6 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Plg in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 96    
    Range % 86-103    
    1:200 Average % 91    
    Range % 83-99    
    1:400 Average % 95    
    Range % 82-97    
    1:800 Average % 95    
    Range % 93-100    
  • 回收率：
    The recovery of rat Plg spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 96 91-102    
    EDTA plasma (n=4) 94 85-99    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    4000 0.121 0.116 0.119      
    2000 0.223 0.230 0.227      
    1000 0.468 0.475 0.472      
    500 0.774 0.785 0.78      
    250 1.225 1.213 1.219      
    125 1.689 1.674 1.682      
    62.5 2.162 2.179 2.171      
    0 2.608 2.594 2.601      
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 最新研究進展：
    Plg是一種廣泛存在于人體內的血漿蛋白，在多種疾病的發生和發展中可能發揮著重要的作用。最近的研究表明，Plg參與了腫瘤生長、血管生成、轉移和免疫逃逸等多個方面的調節，并在心血管疾病、肺部疾病和肝臟疾病中的作用備受關注。對Plg結構和功能的研究也取得了重要進展，這將有助于更深入地了解Plg的生物學特性，為新藥物的開發和治療策略的制定提供更可靠的理論基礎。
  • 功能：
    Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.; Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.
  • 基因功能參考文獻：
    1. Angiostatin overexpression was associated with attenuated renal disease progression in a model of chronic kidney injury, likely because of its anti-inflammatory actions. PMID: 18971211
    2. Expression of angiostatin in the ischemic tissues after ischemia-reperfusion in diabetes mellitus + cerebral ischemia group increased significantly. PMID: 19932587
    3. plasmin has a role as an intracellular protease PMID: 12928439
    4. Plasminogen-induced IL-1beta, TNF-alpha, and iNOS mRNA expression in primary cultured rat microglia and BV2 murine microglial cells. PMID: 14651966
    5. Plg gene expression is up-regulated by nerve growth factor PMID: 17663741
    6. Plasmin activates epithelial Na+ channels by cleaving the gamma subunit PMID: 18981180

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family, Plasminogen subfamily
  • 數據庫鏈接：


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