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Rat Phospholipid hydroperoxide glutathione peroxidase, mitochondrial(GPX4) ELISA kit

  • 中文名稱:
    大鼠谷胱甘肽過氧化物酶,線粒體(GPX4)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-EL009869RA
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠谷胱甘肽過氧化物酶,線粒體(GPX4)酶聯(lián)免疫試劑盒(CSB-EL009869RA)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的GPX4含量。GPX4作為一個重要靶點(diǎn),是谷胱甘肽過氧化物酶家族成員。它能催化還原型谷胱甘肽清除脂質(zhì)過氧化物,在調(diào)控細(xì)胞鐵死亡過程中有關(guān)鍵作用。目前對其研究聚焦在通過調(diào)節(jié)GPX4活性干預(yù)鐵死亡,用于腫瘤等疾病治療。試劑盒檢測范圍為4.68 μU/mL-300 μU/mL,試劑盒適用于科研領(lǐng)域,如探究肝臟疾病、神經(jīng)退行性疾病或代謝綜合征等模型中GPX4活性變化,或用于抗氧化藥物篩選及鐵死亡機(jī)制研究。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Gpx4Phospholipid hydroperoxide glutathione peroxidase ELISA kit; PHGPx ELISA kit; EC 1.11.1.12 ELISA kit; Glutathione peroxidase 4 ELISA kit; GPx-4 ELISA kit; GSHPx-4 ELISA kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    4.68 μU/mL-300 μU/mL
  • 靈敏度:
    1.17 μU/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Cancer
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat GPX4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:200Average %85
    Range %80-90
    1:400Average %102
    Range %97-107
    1:800Average %87
    Range %83-91
    1:1600Average %97
    Range %91-103
  • 回收率:
    The recovery of rat GPX4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8380-86
    EDTA plasma (n=4)9287-97
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μU/mlOD1OD2AverageCorrected
    3002.588 2.597 2.593 2.439
    1502.292 2.212 2.252 2.098
    751.614 1.635 1.625 1.471
    37.51.021 1.065 1.043 0.889
    18.750.622 0.638 0.630 0.476
    9.370.384 0.374 0.379 0.225
    4.680.242 0.252 0.247 0.093
    00.152 0.156 0.154
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能:
    Essential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins. Can also reduce fatty acid hydroperoxide, cholesterol hydroperoxide and thymine hydroperoxide. Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation. Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species. The presence of selenocysteine (Sec) versus Cys at the active site is essential for life: it provides resistance to overoxidation and prevents cells against ferroptosis. The presence of Sec at the active site is also essential for the survival of a specific type of parvalbumin-positive interneurons, thereby preventing against fatal epileptic seizures. May be required to protect cells from the toxicity of ingested lipid hydroperoxides. Required for normal sperm development and male fertility. Essential for maturation and survival of photoreceptor cells. Plays a role in a primary T-cell response to viral and parasitic infection by protecting T-cells from ferroptosis and by supporting T-cell expansion. Plays a role of glutathione peroxidase in platelets in the arachidonic acid metabolism. Reduces hydroperoxy ester lipids formed by a 15-lipoxygenase that may play a role as down-regulator of the cellular 15-lipoxygenase pathway.; Specifically able to suppress the production of leukotriene and prostaglandin in response to several stimuli by reducing fatty acid hydroperoxide.; Specifically required to prevent mitochondrial cell death by mediating reduction of cardiolipin hydroperoxide. Also required for normal sperm development and male fertility.; Required for male fertility by stabilizing the condensed chromatin in sperm nuclei.
  • 基因功能參考文獻(xiàn):
    1. Gpx4-catalyzed reduction of lipid hydroperoxides in lipid membranes has been described. PMID: 28709976
    2. MS/MS analysis of oxidized rat GPx4 complies with the predicted structure, an 8-membered ring, in which selenium is bound as selenenylamide to the protein backbone. PMID: 26163004
    3. mitochondria-targeted antioxidant SkQ1 (50 nmol/kg during 5 days) significantly increased the mRNA levels of Nrf2 transcription factor and Nrf2-induced genes encoding antioxidant enzymes SOD1, SOD2, CAT, and GPx4 in rat peripheral blood leukocytes. PMID: 26071777
    4. Our findings suggest that a decrease in mitochondrial GPx4 monomer and a functional loss of activity may be a contributing factor to the vulnerability of dopaminergic neurons in Parkinson's disease PMID: 23816523
    5. Rat Gpx4 is a moonlighting protein that functions both as a peroxidase, as well as a structural protein. PMID: 10464096
    6. Upon selenium fluctuation, the modulation of UGA recoding efficiency depends on the nature of the SECIS, with Gpx1 being more sensitive than Gpx4. PMID: 22614831
    7. activity in testis and epididymal spermatozoa of rats of different ages (phospholipid hydroperoxide glutathione peroxidase PHGPx, Gpx4). PMID: 11903656
    8. an important component of the mechanisms regulating inflammation PMID: 12397078
    9. gene is differentially spliced during the meiotic prophase and haploid cell phases of spermatogenesis PMID: 12533403
    10. PHGPx signals were detected in chromatoid bodies, clear nucleoplasm, mitochondria-associated material, mitochondrial aggregates, granulated bodies, and vesicles in residual bodies in addition to mitochondria, nuclei, and acrosomes during spermatogenesis. PMID: 12724282
    11. the nuclear PHGPx variant has a role in regulating cell division PMID: 12819198
    12. overexpression provides protection against damage from simulated ischemia/reoxygenation injury, particularly in the mitochondria, and the combination of mPHGPx and HSP60/10 imparts an added protective effect PMID: 14583338
    13. presence of traces of the larger snGPx form suggests that exhaustive proteolytic processing of the precursor produces the 25.9 kDa enzyme PMID: 15670826
    14. PHGPx activity in activated PMNs appears to be related to the appearance of the intrinsic function of PMNs in the inflammatory site. PMID: 15757501
    15. TGR and GPx4 can serve as a novel disulfide bond formation system and contain a catalytic selenocysteine consistent with the role of selenium in male reproduction PMID: 15901730
    16. PHGPx interacts with SMCP; the cysteine motif of PHGPx is a new substrate of the selenoperoxidase PMID: 16159880
    17. Astrocytic upregulation of GPx4 in response to injury is part of a protective cascade counteracting further cell damage in brain injury. PMID: 17603929
    18. We therefore propose that under persistent oxidative stress the formation of hepoxilin A3 and the HXA3-induced upregulation of PHGPx constitute a compensatory defense response to protect the vitality and functionality of the cell. PMID: 17997296
    19. liver and kidney Gpx4 activity in old female rats PMID: 19398061
    20. Data show that the intake of fish oil in the selenium-deficient animal led to a markedly altered formation of sperm nuclei glutathione peroxidase (snGPx), also designated as the nuclear form of PHGPx (n-PHGPx). PMID: 19723078

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  • 亞細(xì)胞定位:
    [Isoform Nuclear]: Nucleus. Nucleus, nucleolus.; [Isoform Mitochondrial]: Mitochondrion. Cytoplasm.; [Isoform Cytoplasmic]: Cytoplasm.
  • 蛋白家族:
    Glutathione peroxidase family
  • 組織特異性:
    Present primarily in testis. Expressed in flagella of epididymal sperm. Isoform Cytoplasmic: Highly expressed in testis. Present in spermatogonia, spermatocyte and spermatid (at protein level).
  • 數(shù)據(jù)庫鏈接:

    KEGG: rno:29328

    UniGene: Rn.3647



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