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  4. Rat Osteocalcin/Bone gla protein,OT/BGP ELISA kit

Rat Osteocalcin/Bone gla protein,OT/BGP ELISA kit

  • 中文名稱：
    大鼠骨鈣素/骨谷氨酸蛋白(OT/BGP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E05129r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠骨鈣素/骨谷氨酸蛋白(OT/BGP)酶聯(lián)免疫試劑盒（CSB-E05129r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿,組織勻漿.樣本中的BGLAP含量。BGLAP是重要靶點。它與骨代謝密切相關(guān)，在成骨細(xì)胞中表達(dá)，是反映骨形成的特異指標(biāo)。研究機(jī)制方面，聚焦其在骨發(fā)育、骨重建等進(jìn)程中的作用，探索調(diào)節(jié)它的信號通路，有望為骨相關(guān)疾病如骨質(zhì)疏松癥等治療提供新思路。試劑盒檢測范圍為125 pg/mL- 8000 pg/mL，該產(chǎn)品適用于骨代謝基礎(chǔ)研究、骨質(zhì)疏松動物模型評估、骨修復(fù)藥物篩選等科研場景，為探究骨生物學(xué)機(jī)制及藥物干預(yù)效果提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Bglap ELISA Kit; Bglap2 ELISA Kit; Osteocalcin ELISA Kit; Bone Gla protein ELISA Kit; BGP ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma,tissue homogenates.
  • 檢測范圍：
    125 pg/mL- 8000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat OT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    102

    Range %

    98-107

    1:2

    Average %

    97

    Range %

    94-101

    1:4

    Average %

    97

    Range %

    92-102

    1:8

    Average %

    96

    Range %

    89-101

  • 回收率：

    The recovery of rat OT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    92

    88-96

    EDTA plasma (n=4)

    95

    91-99

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    8000

    1.947

    1.834

    1.891

    1.713

    4000

    1.218

    1.167

    1.193

    1.015

    2000

    0.682

    0.658

    0.670

    0.493

    1000

    0.453

    0.429

    0.441

    0.264

    500

    0.292

    0.301

    0.296

    0.119

    250

    0.233

    0.243

    0.238

    0.061

    125

    0.202

    0.194

    0.198

    0.020

    0

    0.180

    0.175

    0.177

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

相關(guān)問答

■ 常見問題解答
Q:

I would like to know what wavelength this product needs to be read at to make sure we have the appropriate filters. Also, we have some mouse cells lines, will this product work for those cell lines as well?

A:
Thanks for your inquiry.
We use TMB substrate. The detection wavelength must be 450nm. The absorbance value will be very good at this wavelength. Considering there may be scratches at the bottom of the vial when you add samples, we suggest you use dual wavelength( detection wavelength and correction wavelength).
We don't suggest you use this kit to test mouse cells lines. If you want to test it, you can do a pretest first.

靶點詳情

  • 功能：
    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • 基因功能參考文獻(xiàn)：
    1. These data suggest that Undercarboxylated osteocalcin may have other effects on skeletal muscle in addition to its insulin sensitizing effect. PMID: 27291707
    2. Uncarboxylated osteocalcin potentiates insulin secretion, inhibits Kv channels and increases [Ca(2+)]i. PMID: 27746193
    3. This rat model of complete loss of osteocalcin provides a platform for further understanding the role of osteocalcin in disease. PMID: 27483347
    4. gamma-Carboxylase inhibition prevents osteocalcin carboxylation and protects against diabetic cardiomyopathy, possibly through direct action on upregulated G protein-coupled receptor family C group 6 member A and indirectly via adiponectin. PMID: 27488359
    5. Bone Gla Protein PMID: 25185647
    6. In primary adipocytes, both carboxylated and uncarboxylated osteocalcin increased basal and insulin-stimulated glucose transport. PMID: 24554534
    7. The physiological changes associated with estrous cycle stage were not sufficient to cause a detectable difference in carboxylated or undercarboxylated osteocalcin concentrations in this study. PMID: 23817840
    8. Osteocalcin (OC)-containing neurons are distributed throughout the cerebellar nuclei; OC may play a role in modulating the neuroprotective function of osteopontin. PMID: 22552891
    9. warfarin impaired the carboxylation of osteocalcin in rats. Factor Xa inhibitor edoxaban at or higher doses than needed for an antithrombotic effect sustained the circulating Gla-osteocalcin level. PMID: 22999414
    10. results suggest that under some physiological conditions the oncoprotein MDM2 may cooperate with p53 to regulate the osteocalcin gene during osteoblastic differentiation. PMID: 22405968
    11. These data are strongly in support of Sp1 as an essential transcription factor required for Osx recruitment and transactivation of the OCN promoter. PMID: 21820092
    12. purified Bone Gla Protein (BGP) resulted in stabilization of hypoxia-inducible factor 1alpha (HIF-1alpha) in chondrocytes and vascular smooth muscle cells PMID: 21757657
    13. The mechanism of high glucose induced calcification in vascular smooth muscle cells may be due to the increased expression of cbfalpha-1 and OC. PMID: 21302441
    14. functional link between vitamin D-dependent bone tissue-specific transcription and histone acetylation at osteocalcin locus PMID: 11893738
    15. the VDRE in the distal region of the OC gene promoter is refractory to binding of the VDR-RXR complex when organized in a nucleosomal context PMID: 11964167
    16. deltaEF1 suppresses expression of the endogenous osteocalcin gene PMID: 12193549
    17. acetylation of histones H3 and H4 is functionally coupled to the chromatin remodeling events that mediate the developmental induction of osteocalcin gene transcription in bone cells PMID: 12554783
    18. Type I collagen promotes the activity of dentin formation by dental pulp cells by down-regulating Dmp-1 gene expression and up-regulating osteocalcin gene expression. PMID: 12647294
    19. A human vitamin D receptor-glucocorticoid receptor DNA-binding domain chimera is functional on rat osteocalcin vitamin D-responsive element with only conservative change of lys-49 to arg, and of negatively charged glu-53 to basic amino acid (lys or arg). PMID: 12960019
    20. Data show that in osteoblastic cells, the vitamin D receptor interacts directly with Runx2, and that this interaction contributes significantly to vitamin D3-dependent enhancement of the osteocalcin promoter. PMID: 15456860
    21. Results describe gene regulation by Dlx3 in relation to that of Msx2 and Dlx5 during osteoblast differentiation. PMID: 15456894
    22. Ocn is a Nurr1 target gene, which positions Nurr1 in the core of transcriptional factors regulating osteoblastic gene expression PMID: 15485875
    23. OC-IR nerve fibers in the circumvallate papilla mainly originated from the petrosal ganglion. PMID: 15621021
    24. Osteonectin (ON) and osteocalcin (OC) was used as osteoblast markersand assessed using an indirect immunoperoxidase. technique. PMID: 16443258
    25. CEBP-beta-dependent recruitment of SWI/SNF activity is required for chromatin remodeling and transcriptional activity of the bone-specific osteocalcin gene PMID: 16772287
    26. specific protein-DNA and protein-protein interactions that occur within the context of the OC gene promoter in osteoblastic cells stabilize the preferential association of the VDR-SRC-1 complex PMID: 17218095
    27. These results support a model where VDR preferentially recruits SRC-1 to enhance bone-specific OC gene transcription. PMID: 17786964
    28. These data suggest that long-term reduction in bone OC levels may induce the formation of immature bone, which is easily resorbed with changes in bone metabolism such as ovariectomy, and that OC may be one of the factors affecting bone turnover. PMID: 17968486
    29. TFIIA gamma together with ATF4 and Runx2 stimulates osteocalcin promoter activity and endogenous mRNA expression. PMID: 18171674
    30. Biomineralization followed secretion of osteocalcin, which may reflect early osteoblastic differentiation of cultured mesenchymal stem cells under osteoinductive conditions. PMID: 19191495
    31. The Notch-responsive Hes-1 protein is capable of repressing osteocalcin gene transcription in osteoblastic cells through an E-box in the proximal promoter. PMID: 19670267

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Osteocalcin/matrix Gla protein family
  • 數(shù)據(jù)庫鏈接：

    KEGG: rno:25295

    STRING: 10116.ENSRNOP00000026530

    UniGene: Rn.9722



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