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  4. Rat Neurotrophin 4,NT-4 ELISA KIT

Rat Neurotrophin 4,NT-4 ELISA KIT

  • 中文名稱：
    大鼠神經營養因子4(NT-4)酶聯免疫試劑盒
  • 貨號：
    CSB-E04691r
  • 規格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠神經營養因子4(NT-4)酶聯免疫試劑盒（CSB-E04691r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NTF4含量。NTF4（神經生長因子-4）是一種神經營養因子，由NTF4基因編碼，主要通過TrkB受體信號傳導。研究發現，NTF4在神經發生中起關鍵作用，通過調節Stat3和PKB/Akt活性，抑制Il6和Notch信號通路，促進神經干細胞分化為神經元。試劑盒檢測范圍為47 pg/mL-3000 pg/mL，本試劑盒適用于神經發育、損傷修復機制研究，以及神經退行性疾病模型、藥物干預實驗中NT - 4的動態監測，例如腦組織、外周神經或血液樣本的分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Ntf4 ELISA Kit; Nt4 ELISA Kit; Ntf5 ELISA Kit; Neurotrophin-4 ELISA Kit; NT-4 ELISA Kit; Neurotrophin-5 ELISA Kit; NT-5 ELISA Kit; Neutrophic factor 4 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    47 pg/mL-3000 pg/mL
  • 靈敏度：
    11.75 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat NT-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %82-94
    1:2Average %93
    Range %88-98
    1:4Average %96
    Range %90-102
    1:8Average %99
    Range %95-104
  • 回收率：
    The recovery of rat NT-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9690-102
    EDTA plasma (n=4)10095-105
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    30002.268 2.250 2.259 2.150
    15001.845 1.871 1.858 1.749
    7501.464 1.492 1.478 1.369
    3751.135 1.149 1.142 1.033
    187.50.704 0.724 0.714 0.605
    940.438 0.457 0.448 0.339
    470.287 0.294 0.291 0.182
    00.108 0.110 0.109 　
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能：
    Could serve as a target-derived trophic factor for sensory and sympathetic neurons.
  • 基因功能參考文獻：
    1. The neuroprotective effect of NT-4 on retinas cultured under diabetic stress correlates with suppression of PERK kinase and transcription factor CHOP. PMID: 21767604
    2. alteration of NT-4 protein levels and gene expression in brain stem, cerebellum and cerebral hemisphere in context of ecstasy dependence; results show chronic administration of ecstasy significantly increased NT-4 gene expression in these areas of brain PMID: 21273656
    3. NT-4, alone or when co-expressed with brain-derived neurotrophic factor, is processed within and secreted by the constitutive secretory pathway PMID: 12970359
    4. The results indicate that oleic acid behaves as a neurotrophic factor, inducing GAP-43 expression through a PKC-mediated mechanism that is not mediated by other neurotrophic factors but that is strongly synergized by NT-3 and NT-4/5. PMID: 14519521
    5. NT-4 significantly improved slow motor unit recovery, and provides a basis for therapies intended to aid the functional recovery of muscles after denervating injury. PMID: 14622146
    6. BDNF and NT-4 expression could be modulated by activity of spinal circuitry and further support putative involvement of the endogenous neurotrophins in mechanisms of spinal neuroplasticity. PMID: 15193526
    7. Transcellular induction of neuropeptide Y expression by NT4 and BDNF. PMID: 15703301
    8. NT-4 regulates proliferation and differentiation of the dental epithelium and promotes production of the enamel matrix PMID: 18045880
    9. Analysis of neurotrophin expression in dentate gyrus dosen't reveal an expression change of Ntf4 in a genetically hypertensive strain of Wistar rat. PMID: 18957307

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    NGF-beta family
  • 組織特異性：
    Expressed in thymus, muscle, ovary, brain, heart, stomach and kidney. Expressed in both embryo and adult tissues.
  • 數據庫鏈接：


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