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  4. Rat Neurofilament light polypeptide(NEFL) ELISA kit

Rat Neurofilament light polypeptide(NEFL) ELISA kit

  • 中文名稱：
    大鼠神經絲蛋白輕鏈多肽(NEFL)酶聯免疫試劑盒
  • 貨號：
    CSB-EL015688RA
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠神經絲蛋白輕鏈多肽(NEFL)酶聯免疫試劑盒（CSB-EL015688RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NEFL含量。NEFL 即神經絲輕鏈蛋白，是神經元細胞骨架的組成成分。其背景上，它在神經系統疾病中具有重要意義。研究機制方面，血液和腦脊液中的 NEFL 水平變化能反映神經元損傷情況，可作為多種神經退行性疾病早期診斷和病情監測的生物標志物。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，適用于神經科學領域基礎研究，包括中樞神經系統疾病動物模型構建、神經再生機制探索、神經毒性物質評估等場景，尤其可為腦損傷模型、脊髓病變研究及神經保護藥物開發提供可靠的定量檢測方案。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Nefl ELISA Kit; Nf68 ELISA Kit; Nfl ELISA Kit; Neurofilament light polypeptide ELISA Kit; NF-L ELISA Kit; 68 kDa neurofilament protein ELISA Kit; Neurofilament triplet L protein ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat NEFL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %85-98
    1:2Average %107
    Range %97-111
    1:4Average %90
    Range %83-94
    1:8Average %97
    Range %91-102
  • 回收率：
    The recovery of rat NEFL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9082-94
    EDTA plasma (n=4)9991-104
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.516 2.411 2.464 2.287
    2502.052 1.987 2.020 1.843
    1251.297 1.282 1.290 1.113
    62.50.725 0.784 0.755 0.578
    31.20.442 0.437 0.440 0.263
    15.60.338 0.330 0.334 0.157
    7.80.259 0.249 0.254 0.077
    00.178 0.176 0.177
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

相關問答

■ 常見問題解答
Q:

I have a customer inquiring about CSB-EL015688RA. Could you let me know whether any preservatives are contained in each component? Could you also let me know whether it was contained recombinant protein in
this kit? If yes, Please let me know expression system of the recombinant protein.

A:
Thanks for your inquiry!
CSB-EL015688RA
Standard is NS0-derived recombinant protein.
Preservative is liquid proclin 300(1:3000).

靶點詳情

  • 功能：
    Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
  • 基因功能參考文獻：
    1. Furthermore, the effect of CaMKP on self-assembly of NFL was examined. Electron microscopy revealed that CaMKP markedly prevented NFL from forming large filamentous aggregates, suggesting that CaMKP-binding to NFL inhibits its filament association. These findings may provide new insights into a novel mechanism for regulating network formation of neurofilaments during neuronal differentiation. PMID: 27369073
    2. Data suggest that tetrahydropapaveroline (an endogenous catechol) causes oxidative stress resulting in astrocyte/neuronal cell death via generation of reactive oxygen species and modification/aggregation of NF-L (as in neurodegenerative diseases). PMID: 23228886
    3. In cultured cortical neurons, hamartin and tuberin co-localize with neurofilament light chain preferentially in the proximal to central growth cone region PMID: 12226091
    4. The amount of NF-L correlated with the morphological retinal and optic nerve damage in rats PMID: 15193534
    5. Increased neuritic degeneration was observed in neuronal cells overexpressing neurofilament light mutants. PMID: 15857389
    6. 68-kDa neurofilament light chain (NF-L) as identified as a binding protein of amino-terminal PH domain of PLC-gamma1. PMID: 16819285
    7. Rat pituitary cells from the proopiomelanocortin lineage nearly completely lack NF68 immunoreactivity. PMID: 16940710
    8. Data show that as a consequence of RhoA knockdown, the process which expresses neurofilament is markedly induced in C12 cells. PMID: 18556119

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  • 亞細胞定位：
    Cell projection, axon. Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Intermediate filament family
  • 組織特異性：
    Expressed in the dorsal root ganglion neurons (at protein level).
  • 數據庫鏈接：


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