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中文名稱:大鼠神經肽Nesfatin-1酶聯免疫試劑盒
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貨號:CSB-E14378r
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:大鼠神經肽Nesfatin-1酶聯免疫試劑盒(CSB-E14378r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的Nesfatin-1含量。Nesfatin-1是一種由核組蛋白2(NUCB2)衍生而來的肽段,主要在下丘腦和外周組織表達,具有抑制攝食、調節(jié)能量代謝、參與生長發(fā)育和胰島素抵抗等生物學功能。其研究機制涉及調節(jié)下丘腦黑皮質素信號通路、影響食欲、降低血糖、促進胰島素分泌和調節(jié)糖代謝中酶的活性等方面。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,適用于神經生物學、代謝調控機制研究,以及肥胖相關動物模型的病理機制探索;為研究Nesfatin - 1在生理及病理過程中的作用提供可靠工具;適用于實驗室規(guī)模的樣本批量檢測本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:Nesfatin-1
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:15.6 pg/mL-1000 pg/mL
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靈敏度:3.9 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Others
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat nesfatin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 84 Range % 80-92 1:2 Average % 89 Range % 85-95 1:4 Average % 93 Range % 89-99 1:8 Average % 95 Range % 90-100 -
回收率:
The recovery of rat nesfatin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 104 97-109 EDTA plasma (n=4) 97 93-102 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1000 2.795 2.652 2.724 2.573 500 1.857 1.776 1.817 1.666 250 1.004 1.082 1.043 0.892 125 0.654 0.613 0.634 0.483 62.5 0.424 0.430 0.427 0.276 31.2 0.317 0.299 0.308 0.157 15.6 0.226 0.209 0.218 0.067 0 0.154 0.147 0.151 -
數據處理:
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貨期:3-5 working days
引用文獻
- Resveratrol ameliorates learning and memory impairments induced by bilateral hippocampal injection of streptozotocin in mice YD Wei,Neurochemistry international,2023
- Depression-like?Behavior?Induced?by?Nesfatin-1?in?Rats:?Involvement?of?Increased?Immune?Activation?andImbalance?of?Synaptic?Vesicle?Proteins Ge JF. et al,Front Neurosci,2015
- Acute, but not chronic, stress increased the plasma concentration and hypothalamic mRNA expression of NUCB2/nesfatin-1 in rats Ya-Yun Xu. et al,Neuropeptides,2015
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