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  4. Rat Myoglobin,MYO/MB ELISA Kit

Rat Myoglobin,MYO/MB ELISA Kit

  • 中文名稱：
    大鼠肌紅蛋白(MYO/MB)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08049r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠肌紅蛋白(MYO/MB)酶聯(lián)免疫試劑盒（CSB-E08049r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MB含量。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，運動生理學(xué)、肌肉疾病模型或心血管研究等領(lǐng)域；實驗室研究肌肉損傷機制、評估藥物干預(yù)對肌肉組織的影響或探索代謝性疾病相關(guān)生物標(biāo)志物等科研需求；肌肉相關(guān)基礎(chǔ)研究本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Mb ELISA Kit; Myoglobin ELISA Kit
  • 縮寫：
    MB
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 ng/mL-1000 ng/mL
  • 靈敏度：
    3.9 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MYO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %94
    Range %90-100
    1:2Average %92
    Range %87-100
    1:4Average %97
    Range %90-105
    1:8Average %87
    Range %83-96
  • 回收率：
    The recovery of rat MYO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9688-99
    EDTA plasma (n=4)9591-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    10002.064 2.069 2.067 1.922
    5001.743 1.754 1.749 1.604
    2501.355 1.363 1.359 1.214
    1250.954 0.962 0.958 0.813
    62.50.718 0.728 0.723 0.578
    31.250.498 0.502 0.500 0.355
    15.60.337 0.343 0.340 0.195
    00.143 0.146 0.145 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
  • 基因功能參考文獻(xiàn)：
    1. In rhabdomyolytic rats, the level of heme and free iron in cytoplasm and mitochondria of kidney cells is remarkably increased while inhibition of proteolysis results in further elevation of myoglobin content in the renal tissue. PMID: 27329933
    2. After reperfusion, there was no significant increase in dialysate myoglobin concentration. MDL-28170 did not affect dialysate myoglobin concentration during occlusion or after reperfusion. PMID: 24256333
    3. Amphetamine-induced serum myoglobin release is a potential biomarker and possibly a factor in AMPH-induced toxicity processes. PMID: 23608161
    4. bradykinin-induced NO does not interact significantly with cellular Mb to produce an NMR-detectable quantity of metMb in the perfused rat myocardium PMID: 14766675
    5. determination of myoglobin concentration and succinate dehydrogenase activity in serial sections indicate that myoglobin can lead to a substantial reduction (18-60%) of the extracellular oxygen tension required to prevent an anoxic core in muscle cells PMID: 15048578
    6. Mb-facilitated diffusion has a higher poise in skeletal muscle than in myocardium. PMID: 17038435
    7. Moreover, the similar Q10 for the DMb of solution versus cell Mb suggests that any temperature-dependent alteration of the postulated cell matrix does not significantly affect protein mobility. PMID: 17218454
    8. The nitrite reductase activity of deoxymyoglobin reduces nitrite approximately 36 times faster than deoxyhemoglobin because of its lower heme redox potential. PMID: 17293481
    9. intermittent hypobaric hypoxia decreased myocardial citrate synthase activity and myoglobin and decreased myocardial lactate dehydrogenase activity PMID: 19308875
    10. Data suggest that rhabdomyolysis-induced kidney damage involves direct interaction of myoglobin with mitochondria possibly resulting in iron ions release from myoglobin's heme, which promotes the peroxidation of mitochondrial membranes. PMID: 19545623

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  • 蛋白家族：
    Globin family
  • 數(shù)據(jù)庫鏈接：


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