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  4. Rat Lecithin Cholesterol Acyltransferase(LCAT) ELISA Kit

Rat Lecithin Cholesterol Acyltransferase(LCAT) ELISA Kit

  • 中文名稱：
    大鼠卵磷脂膽固醇酰基轉移酶(LCAT)酶聯免疫試劑盒
  • 貨號：
    CSB-E14201r
  • 規格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠卵磷脂膽固醇酰基轉移酶(LCAT)酶聯免疫試劑盒（CSB-E14201r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LCAT含量。LCAT即卵磷脂膽固醇酰基轉移酶，在膽固醇逆向轉運中發揮關鍵作用。它能催化卵磷脂上的脂肪酸轉移到膽固醇上形成膽固醇酯，利于膽固醇轉運與代謝。相關研究圍繞其在動脈粥樣硬化等疾病中的作用機制，以探索潛在治療靶點。試劑盒檢測范圍為1.87 ng/mL-120 ng/mL，本產品可廣泛應用于基礎科研領域，例如研究高脂血癥、動脈粥樣硬化等疾病模型中LCAT的動態變化，評估藥物或基因干預對脂代謝調控的影響，以及探索膽固醇代謝相關分子機制。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    LcatPhosphatidylcholine-sterol acyltransferase ELISA kit; EC 2.3.1.43 ELISA kit; Lecithin-cholesterol acyltransferase ELISA kit; Phospholipid-cholesterol acyltransferase ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.87 ng/mL-120 ng/mL
  • 靈敏度：
    0.94 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat LCAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %102
    Range %92-107
    1:200Average %88
    Range %84-93
    1:400Average %101
    Range %94-105
    1:800Average %102
    Range %92-105
  • 回收率：
    The recovery of rat LCAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-96
    EDTA plasma (n=4)10292-107
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    1200.075 0.079 0.077
    600.172 0.178 0.175
    300.374 0.382 0.378
    150.634 0.624 0.629
    7.51.178 1.146 1.162
    3.751.504 1.533 1.519
    1.871.673 1.622 1.648
    02.155 2.078 2.117
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Catalyzes the hydrolysis of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor or PAF) to 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF). Also catalyzes the transfer of the acetate group from PAF to 1-hexadecanoyl-sn-glycero-3-phosphocholine forming lyso-PAF. Catalyzes the esterification of (24S)-hydroxycholesterol (24(S)OH-C), also known as cerebrosterol to produce 24(S)OH-C monoesters.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    AB hydrolase superfamily, Lipase family
  • 組織特異性：
    Detected in blood plasma (at protein level).
  • 數據庫鏈接：


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