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中文名稱:大鼠層連蛋白/板層素(LN)酶聯免疫試劑盒
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貨號:CSB-E04646r
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規格:96T/48T
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價格:¥3900/¥2500
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其他:
產品詳情
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產品描述:大鼠層連蛋白/板層素(LN)酶聯免疫試劑盒(CSB-E04646r)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的LN含量。大鼠層連蛋白/板層素(LN)是細胞外基質的主要結構成分,對基膜的組裝起關鍵作用。其在細胞表面形成網絡結構,將細胞固定在基膜上,參與多種生理過程,如細胞粘附、增殖和分化。研究顯示,LN在肝硬化等疾病中表達增加,可能與肝竇毛細血管化形成有關。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL,適用于基礎醫學研究中LN相關分子機制探索,如肝/肺纖維化模型評估、傷口愈合過程監測、腫瘤侵襲性研究等,為體外實驗提供可靠的蛋白定量工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:N/A
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縮寫:LN
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma
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檢測范圍:62.5 pg/mL-4000 pg/mL
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靈敏度:15.6 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Signal Transduction
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat LN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 91 Range % 87-99 1:200 Average % 97 Range % 93-105 1:400 Average % 85 Range % 82-89 1:800 Average % 90 Range % 85-98 -
回收率:
The recovery of rat LN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 85-95 EDTA plasma (n=4) 95 89-99 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 4000 0.069 0.075 0.072 2000 0.124 0.125 0.125 1000 0.205 0.223 0.214 500 0.361 0.340 0.351 250 0.593 0.624 0.609 125 0.821 0.858 0.840 62.5 1.119 1.116 1.118 0 2.165 2.216 2.191 -
數據處理:
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貨期:3-5 working days
引用文獻
- Effects of novel Fufang Biejia Ruangan Tablets with sheep placenta as substitute for Hominis Placenta on CCl4-induced liver fibrosis B Shen,Chinese Herbal Medicines,2023
- Comprehensive analysis of transcriptomics and metabolomics to illustrate the underlying mechanism of helenalin against hepatic fibrosis Yuhua Xiong,European Journal of Pharmacology,2022
- A Compound of Chinese Herbs Protects against Alcoholic Liver Fibrosis in Rats via the TGF-β1/Smad Signaling Pathway Xiaomeng Li,Evidence-Based Complementary and Alternative Medicine,2019
- Role of betaine in liver injury induced by the exposure to ionizing radiation Shedid SM.et al,environmental toxicology,2018
- Hepatoprotective effects of Dicliptera chinensis polysaccharides on dimethylnitrosamine-induced hepatic fibrosis rats and its underlying mechanism Zhang K. et al,J Ethnopharmacol,2015
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