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  4. Rat Keratin, type I cytoskeletal 18(KRT18) ELISA kit

Rat Keratin, type I cytoskeletal 18(KRT18) ELISA kit

  • 中文名稱：
    大鼠細胞角蛋白18-M65（CK 18-M65）酶聯免疫試劑盒
  • 貨號：
    CSB-EQ027319RA
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠細胞角蛋白18-M65（CK 18-M65）酶聯免疫試劑盒（CSB-EQ027319RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿,組織勻漿樣本中的KRT18含量。KRT18 是角蛋白家族成員。在細胞中維持細胞結構穩定、參與信號傳導。其異常表達與腫瘤等疾病密切相關。研究發現它可作為腫瘤診斷標志物，還參與腫瘤細胞增殖、遷移等過程，有望成為潛在治療靶點。試劑盒檢測范圍為0.94 ng/mL-60 ng/mL，適用于科研領域中對大鼠模型的組織病理機制研究，例如肝纖維化、肝癌模型構建或化學誘導毒性實驗中細胞死亡動態監測，為評估上皮細胞損傷程度及疾病進展提供定量分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Krt18 ELISA Kit; Krt1-18Keratin ELISA Kit; type I cytoskeletal 18 ELISA Kit; Cytokeratin-18 ELISA Kit; CK-18 ELISA Kit; Keratin-18 ELISA Kit; K18 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma,tissue homogenates
  • 檢測范圍：
    0.94 ng/mL-60 ng/mL
  • 靈敏度：
    0.23 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat KRT18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    96

    Range %

    92-100

    1:2

    Average %

    99

    Range %

    94-103

    1:4

    Average %

    101

    Range %

    98-105

    1:8

    Average %

    98

    Range %

    93-104

  • 回收率：

    The recovery of rat KRT18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    89

    84-95

    EDTA plasma (n=4)

    100

    95-105

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    60

    2.606

    2.657

    2.632

    2.469

    30

    1.798

    1.774

    1.786

    1.623

    15

    1.152

    1.198

    1.175

    1.012

    7.5

    0.713

    0.739

    0.726

    0.563

    3.75

    0.446

    0.468

    0.457

    0.294

    1.87

    0.334

    0.348

    0.341

    0.178

    0.94

    0.265

    0.267

    0.266

    0.103

    0

    0.161

    0.165

    0.163

    　

  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

相關問答

■ 常見問題解答
Q:

1. Which is this standard in this kit, natural material or recombinant? If recombinant, please inform me on the expression method (E.coli etc.).
2. Does this kit include polyclonal antibody (except for microplate binding protein)? If so, please tell me the derivation and purification method.
3. Is the standard or antibody, including any additive agent (antiseptic agent or preserving agent)? If so, please let me know the composition and concentration.
4. Does this product include a Stop solution? If so, please tell me the composition and concentration for the stop solution.
5. Please let me know whether this kit include any hazardous substances (azide, thimerosal, 2-ME, etc). If so, please inform me of the composition and concentration.

A:
Thanks for your inquiry!
Pls check our answer:
1, Standard of CSB-EQ027319RA is NS0-derived recombinant protein
2, Coated anti: Mouse monoclonal antibody(precoated in the plate); Detection anti: Goat polyclonal(affinity purification)
3, Stop solution: 4N sulfuric acid
4, There is no hazardous substances (azide, thimerosal, 2-ME, etc) included in the kit.
5, Preservative of this kit is proclin 300(1:3000).

靶點詳情

  • 功能：
    When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells.
  • 基因功能參考文獻：
    1. Resutls show that the appearance caspase-cleaved CK18 in plasma was found to reflect formation of the caspase-cleaved epitope in PMID: 19407366
  • 亞細胞定位：
    Nucleus matrix. Nucleus, nucleolus. Cytoplasm.
  • 蛋白家族：
    Intermediate filament family
  • 組織特異性：
    Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine
  • 數據庫鏈接：


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