在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Rat Interleukin 13,IL-13 ELISA KIT

Rat Interleukin 13,IL-13 ELISA KIT

  • 中文名稱：
    大鼠白介素13(IL-13)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07454r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠白介素13(IL-13)酶聯(lián)免疫試劑盒（CSB-E07454r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IL13含量。IL-13是一種由T細(xì)胞、肥大細(xì)胞等分泌的細(xì)胞因子。它在過敏反應(yīng)、組織纖維化等病理過程中發(fā)揮重要作用。研究機制方面，IL-13通過與其受體結(jié)合，激活下游信號通路，調(diào)控基因表達(dá)，進(jìn)而影響細(xì)胞增殖、分化及炎癥因子釋放等。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，該產(chǎn)品適用于探索Th2型免疫調(diào)控機制、評估藥物干預(yù)對炎癥通路的影響，或通過檢測實驗動物模型（如哮喘或寄生蟲感染模型）中IL - 13的動態(tài)變化，揭示其與病理進(jìn)程的關(guān)聯(lián)性。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Il13 ELISA Kit; Il-13 ELISA Kit; Interleukin-13 ELISA Kit; IL-13 ELISA Kit; T-cell activation protein P600 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %91
    Range %88-93
    1:2Average %104
    Range %99-107
    1:4Average %93
    Range %88-97
    1:8Average %99
    Range %91-103
  • 回收率：
    The recovery of rat IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9491-96
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5001.854 1.766 1.810 1.727
    2501.117 1.106 1.112 1.029
    1250.728 0.743 0.736 0.653
    62.50.517 0.534 0.526 0.443
    31.20.303 0.312 0.308 0.225
    15.60.210 0.202 0.206 0.123
    7.80.171 0.164 0.168 0.085
    00.084 0.082 0.083 　
  • 本試劑盒所含材料：
    • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat IL-13 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled IL-13 antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
    • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
    • One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
    • An instruction manual

    顯示更多

    收起更多

  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

    顯示更多

    收起更多

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

靶點詳情

  • 功能：
    Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages.
  • 基因功能參考文獻(xiàn)：
    1. SUMF2 may mediate airway inflammation in allergic asthma by modulating the expression of IL-13 PMID: 26722390
    2. IL-13 significantly enhanced C/EBP-alpha/COX-2 expression and PGE2 production in LPS-treated microglial cells. Paradoxically, IL-13 abolished C/EBP-beta/PPAR-gamma/HO-1 expression. PMID: 23881867
    3. asthma the ERK expression and activation levels were increased, as was the protein level of IL-13. The ERK signaling pathway may be involved in the increased expression of the Th2 cytokine IL-13 in asthma. PMID: 20819635
    4. The IL-13 system is a novel regulator of paSMC growth. Dysregulation of IL-13 receptor expression in IPAH may partially underlie smooth muscle hypertrophy associated with pathological vascular remodeling in IPAH. PMID: 20522789
    5. IL-13 is involved in tobacco smoke-induced changes in the structure and function of rat intrapulmonary airways PMID: 19783789
    6. This finding provides an unequivocal evidence of IL-13 participation in pancreatic fibrosis, illustrating a new strategy for chronic pancreatitis. PMID: 20100461
    7. Inhibition of HO-1 with tin protoporphyrin reversed cytoprotective/antiapoptotic effects of IL-13 in liver transplantation. PMID: 12919086
    8. IL-13 may control brain inflammation by inducing the death of activated microglia in vivo, resulting in an enhancement of neuronal survival. PMID: 15042582
    9. study demonstrated at the protein and mRNA levels, both in vivo and in vitro, that alveolar macrophages produce IL-25 and IL-13 in response to fine particles of TiO2 PMID: 15961726
    10. IL-13 overexpression in the rat could lead to podocyte injury and minimal-change-like nephropathy. PMID: 17429054
    11. The findings support a role for IL-13 as an in vivo antiangiogenic factor and provide a rationale for its use in RA to control. pathologic neovascularization. PMID: 17665443
    12. IL-13 down-regulates the cytokine-induced iNOS transcription by decreasing iNOS specific IRF-1/ISRE binding activity PMID: 17723228
    13. expression decreased in lung in response to alcohol exposure PMID: 19120067
    14. Endogenous IL-13 triggers microglial NADPH oxidase-derived oxidative stress, leading to degeneration of hippocampal neurons in vivo. PMID: 19752235

    顯示更多

    收起更多

  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-4/IL-13 family
  • 數(shù)據(jù)庫鏈接：


主站蜘蛛池模板： 九个美女露脸撒尿嘘嘘视频| 亚洲加勒比无码一区二区| 精品一区二区三区无码视频| 日本道色综合久久影院| 久久天天躁夜夜躁狠狠综合| av无码不卡一区二区三区| 婷婷丁香五月中文字幕| 亚洲熟女综合一区二区三区| 亚洲日韩色在线影院性色| 国产精品9999久久久久| 午夜福利理论片在线观看播放| 日韩av无码久久一区二区| 99r在线精品视频在线播放| 亚洲国产欧美在线人成app| 性做久久久久久免费观看| 国产成人无码a区视频| 国产精品a久久777777| 亚洲欧美色中文字幕在线| 99国产精品自在自在久久| 免费看成人午夜福利专区| 无码帝国www无码专区色综合| 性欧美欧美巨大69| 丰满少妇被猛烈进入| 狠狠亚洲色一日本高清色| 99久久人妻精品免费一区| 久久99成人免费| 熟女毛多熟妇人妻在线视频| 性亚洲videofree高清| 国产男女猛烈无遮挡a片漫画| 狠狠色噜噜狼狼狼色综合久| 18禁止观看强奷免费国产大片| 久久久久99精品成人片试看| 手机看片福利一区二区三区| 99视频有精品视频高清| 一道久在线无码加勒比| 日韩va中文字幕无码电影| 亚洲精品色无码av试看| 日本久久久久久级做爰片| 人妻丰满av无码久久不卡| 黑人狠狠的挺身进入| 日日碰狠狠添天天爽五月婷|