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  4. Rat Interleukin 1β,IL-1β ELISA Kit

Rat Interleukin 1β,IL-1β ELISA Kit

  • 中文名稱：
    大鼠白介素1β (IL-1β)酶聯免疫試劑盒
  • 貨號：
    CSB-E08055r-IS
  • 規格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠白介素1β (IL-1β)酶聯免疫試劑盒（CSB-E08055r-IS）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL1B含量。IL1B 是重要的細胞因子。在炎癥反應等生理病理過程中發揮關鍵作用。研究發現它可激活下游炎癥信號通路，誘導多種炎癥介質產生。針對 IL1B 研發藥物，干預其作用機制，有望為炎癥相關疾病如類風濕關節炎等治療提供新策略。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，靈敏度為15.6 pg/mL。常用于評估感染性疾病、自身免疫病及神經退行性病變等研究模型；廣泛用于炎癥機制研究、藥物抗炎效果評價、免疫調控實驗及動物疾病模型構建，特別適用于腦、肝臟等組織勻漿中細胞因子釋放的動態監測。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Il1bInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    91

    Range %

    91-105

    1:2

    Average %

    102

    Range %

    95-109

    1:4

    Average %

    99

    Range %

    92-105

    1:8

    Average %

    93

    Range %

    86-98

  • 回收率：

    The recovery of rat IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    92

    85-98

    EDTA plasma (n=4)

    97

    90-100

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    2000

    2.826

    2.634

    2.730

    2.569

    1000

    2.045

    1.967

    2.006

    1.845

    500

    1.349

    1.328

    1.339

    1.177

    250

    0.818

    0.829

    0.824

    0.662

    125

    0.474

    0.491

    0.482

    0.321

    62.5

    0.344

    0.353

    0.349

    0.187

    31.25

    0.238

    0.244

    0.241

    0.080

    0

    0.157

    0.165

    0.161

    　

  • 數據處理：
  • 貨期：
    3-5 working days

靶點詳情

  • 功能：
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
  • 亞細胞定位：
    Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
  • 蛋白家族：
    IL-1 family
  • 數據庫鏈接：


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