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Rat Interferon γ,IFN-γ ELISA Kit

  • 中文名稱:
    大鼠γ干擾素(IFN-γ)酶聯免疫試劑盒
  • 貨號:
    CSB-E04579r
  • 規格:
    96T/48T
  • 價格:
    ¥3900/¥2500
  • 其他:

產品詳情

  • 產品描述:
    大鼠γ干擾素(IFN-γ)酶聯免疫試劑盒(CSB-E04579r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IFNG含量。IFNG即干擾素-γ,是一種多功能細胞因子。它在免疫調節中至關重要,能增強巨噬細胞和NK細胞活性、促進Th1細胞分化。研究機制方面,通過與受體結合激活下游信號通路,調控免疫相關基因表達,在抗感染、抗腫瘤等領域是重要研究靶點。試劑盒檢測范圍為0.625 pg/mL-40 pg/mL,適用于科研領域研究感染性疾病模型、自身免疫疾病機制、腫瘤免疫微環境分析及藥物干預實驗中的免疫動態變化監測,為探索Th1型免疫反應、炎癥調控及免疫治療策略提供可靠數據支持本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    IfngInterferon gamma ELISA Kit; IFN-gamma ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.625 pg/mL-40 pg/mL
  • 靈敏度:
    0.156 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %92
    Range %88-95
    1:2Average %104
    Range %99-107
    1:4Average %93
    Range %87-96
    1:8Average %99
    Range %93-103
  • 回收率:
    The recovery of rat IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)9692-98
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    402.361 2.224 2.293 2.103
    201.430 1.376 1.403 1.213
    100.916 0.909 0.913 0.723
    50.617 0.626 0.622 0.432
    2.50.424 0.453 0.439 0.249
    1.250.333 0.340 0.337 0.147
    0.6250.272 0.288 0.280 0.090
    00.192 0.188 0.190  
  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能:
    Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
  • 基因功能參考文獻:
    1. Lipopolysaccharide enhances TLR4 expression in rat pulmonary artery smooth muscle cells and induced production of IFNgamma dramatically. PMID: 28714001
    2. This study suggested that IFN-gamma maintained tolerance mediating alloantigen-specific CD4(+)CD25(+)T cells. PMID: 28487237
    3. Addition of 20 (3) and 40 (6) mg/L (mg/kg BW) of boron to drinking water significantly increased rat serum IgG concentrations, splenic IFN-gamma and IL-4 expression as well as the number of splenic CD3(+), CD4(+) and proliferating cell nuclear antigen (PCNA)(+) cells. PMID: 28092075
    4. The results provide evidence that changes in IFN-gamma expression and signalling may be perceived at stages preceding refractory cachexia, and therefore, might be employed as a means to assess the early stage of the syndrome PMID: 26987263
    5. This study found that ACZ could decrease the content of pro-inflammatory cytokines, such as MCP-1, IL-1beta, TNF-alpha and IFN-gamma in rat lungs, and, the lung injury in the HA+ACZ group reduced. PMID: 27104804
    6. IFN-gamma dose-dependently induces HMGB1 cytoplasmic accumulation and its active release from VSMCs, resulting in enhanced HMGB1 in the medium. PMID: 27579780
    7. The results thus demonstrate that XO mediates oxidative stress in MB+PQ-treated rat PMNs via iNOS-independent but cytokine (predominantly IFN-gamma)-dependent mechanism. PMID: 28025796
    8. Iba-1-immunoreactive microglia are activated and IFN-gamma and IL-1beta levels are increased in the hippocampal CA1 region and the dentate gyrus of aged, diabetic rats, indicating that chronic diabetes may accelerate aging processes in the hippocampus PMID: 24571083
    9. The findings of the present study suggest that part of ischemic injury in the embolic stroke may be mediated through the increased levels of inflammatory cytokines. PMID: 24338258
    10. Apoptotic cell death assessed by TUNEL assay was enhanced in both ERalpha and ERbeta-silenced VSC4.1 motoneurons following IFN-gamma and IGF-1 exposure. PMID: 24188094
    11. The interleukin (IL)-18/IFN-gamma system may be involved in status epilepticus-induced vasogenic edema formation. PMID: 22338606
    12. IFNgamma produced by constituents of the mucosal immune system modulates epithelial cell functions with relevance for intestinal wound healing and may play a role in preserving the integrity of the intestinal epithelium following various forms of injuries. PMID: 21385594
    13. Furthermore, IFNgamma reduced JNK phosphorylation, which tightly was associated with decreased phosphorylation of downstream factors c-Jun and Smad3 and decreased binding activity of c-Jun and Smad3 in the preprpET-1 promoter. PMID: 22301113
    14. Study provides mechanistic insights in the IFN-mediated suppression of ICP4 and IE180 in sensory neuronal cells. PMID: 21641126
    15. Garlicin could increase the level of INF-gamma and decrease the level of IL4 significantly in allergic rhinitis. PMID: 18826099
    16. IFN-gamma is able to activate rat intestinal mucous microvascular endothelial cells, thereby leading to upregulated expressions of IFN-alpha/beta. PMID: 20214528
    17. IFN-gamma enhanced IL-27 secretion, revealing a cooperative interplay between the two cytokines. PMID: 21123181
    18. Immunoregulatory cytokines belonging to the Th-1 group (IL-2 and IFN-gamma) were increased in the retina of experimental diabetic rats PMID: 20213480
    19. The interplay between IFNgamma released from immune cells and PACAP is of importance in brain inflammation and may affect the regeneration and recruitment of NPCs in immune diseases PMID: 20559421
    20. Fasting-induced iNOS activation in epithelial cells may induce apoptosis mediatiors such as interferon-gamma via a reactive oxyge speces-mediated mechanism. PMID: 20378828
    21. Data show that IgG stimulation induced a significant TLR4 expression and TNF-alpha secretion in cultured microglial cells in a dose-dependent manner, while IFN-gamma was PMID: 19257980
    22. Data show that treated rats exhibited the lowest degree of inflammatory infiltration of the spinal cord, as well as the lowest levels of nuclear factor kappa B, interleukin-12 and interferon-gamma. PMID: 19922500
    23. Data show that methylprednisolone inhibited IFN-gamma and IL-17 expression and production in cells isolated from the CNS of rats with experimental autoimmune encephalomyelitis. PMID: 20003332
    24. role of IFN-gamma in the regulation of bacterial load in the cecal ligation and puncture (CLP) model of intra-abdominal sepsis in the rat PMID: 11770039
    25. Interferon-gamma significantly inhibits hepatic stellate cell proliferation and reduces smooth muscle alpha-actin abundance after 6 days treatment. PMID: 11940252
    26. the spleen contributes to the endotoxin-induced liver injury by modulating release of IFNgamma PMID: 11954833
    27. IFN-gamma increased mRNA activity and gene expression of TGase 2 in a rat small intestinal epithelial cell line. PMID: 12162878
    28. IFN-gamma inhibited TGF-beta(1)-induced increases in PG expression by fibroblasts in fibrotic lungs. PMID: 12225958
    29. Increased levels in BALF of anti-IP-10 antibody-treated F344 rats following Sendai viral infection. PMID: 12581489
    30. role in post-translational regulation of Muc4 PMID: 12668667
    31. plays an important physiological role during inflammation by down-regulating collagen gene expression and activating major histocompatibility II complex PMID: 12968017
    32. Differential signaling through IFN-gamma in the conjunctiva between the Lewis and Brown Norway rat strains in experimental allergic conjunctivitis. This may be due to differences in histopathological character between the two strains. PMID: 14605445
    33. The protein level of IFN-gamma follows a physiological circadian rhythm in rat splenocytes that concurrently drives the circadian rhythm of natural killer cell cytolytic activity. PMID: 14978081
    34. IFN-gamma induces rapid secretion of macrophage migration inhibitory factor by renal tubular epithelial cells. PMID: 15012733
    35. JNK signaling plays an important role in the inhibitory effects of cAMP on IL-1beta+IFNgamma-induced iNOS gene expression in cultured hepatocytes PMID: 15115662
    36. Taken together, these results suggest that NDGA regulates IFN-gamma-mediated inflammation through mechanisms unrelated to the inhibition of 5-LO PMID: 15694390
    37. retinoic acid induces an anti-inflammatory effect by suppressing the activation of the JAK/STAT pathway in IFN-gamma-treated astrocytes PMID: 15721283
    38. IFN-gamma, in conjunction with TNF or LPS, can both inhibit hepatocyte proliferation through the generation of nitric oxide and stimulate oval cell replication. PMID: 15799032
    39. Circadian expression of interferon gamma mRNA is demonstrated for the first time in natural killer (NK) cells enriched from rat spleen. PMID: 15944262
    40. IFN-gamma can modulate the expression of RT1A and RT1-DM during the whole pregnancy PMID: 15946996
    41. IFN-gamma in NK cells has a role in the link between the innate and adaptive immune responses early after transplantation PMID: 16095488
    42. The leukemia inhibitory factor inhibition of interferon-gamma toxicity in oligodendrocytes is mediated by a suppressor of cytokine signaling-3 independent mechanism. PMID: 16289836
    43. The evidence presented suggests that IGF-1 acts to antagonize the IFNgamma-induced microglial activation, the accompanying increase in IL-1beta concentration and the consequent deficit in LTP. PMID: 16464236
    44. Intermittent fasting dietary restriction stimulates IFN-gamma-mediated neuroprotective signaling in the hippocampus, suggesting a role for this cytokine in neural cytoprotection. PMID: 16521127
    45. Astrocytes up-regulated IFN-gamma gene expression and protein synthesis in T cells, which coincided with astrocytes' ability to express IL-23 subunit p19 and common IL-12/IL-23 subunit p40. PMID: 17969033
    46. the primary effect of atorvastatin is to increase IL-4, which antagonizes the effects of IFNgamma, the associated increase in microglial activation, and the subsequent cascade of events. PMID: 17981803
    47. Innate immunity (natural killer cells/IFN-gamma) is activated after allogeneic transplantation, which likely contributes to liver injury and inhibits hepatocyte proliferation. PMID: 18292182
    48. Data suggest that estradiol effects on T cell IFN-gamma and IL-2 production afer trauma-hemorrhage are mediated via non-genomic pathway, and which is in turn mediated via ERK, p38, and JNK pathways. PMID: 18343154
    49. The aim of this study was to evaluate the constitutive expression of IL-18 and its receptors (IL-18Ralpha and IL-18Rbeta) in intestinal epithelial cells (IEC) stimulated by tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma). PMID: 18547159
    50. An enhanced integrated stress response could promote oligodendrocyte survival in immune-mediated demyelination diseases. PMID: 18818381

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  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Type II (or gamma) interferon family
  • 組織特異性:
    Released primarily from activated T lymphocytes.
  • 數據庫鏈接:


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