Rat Interferon β,IFN-β/IFNB ELISA Kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠β干擾素(IFN-β/IFNB)酶聯免疫試劑盒（CSB-E04845r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFNB1含量。IFNB1即干擾素β1，它是一種細胞因子，在免疫調節中發揮關鍵作用。研究發現它能抑制病毒復制、調節免疫細胞活性。在醫學上常用于多發性硬化癥等疾病治療，其機制是通過與受體結合，激活下游信號通路，發揮免疫調節和抗病毒等效應。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，本試劑盒可為病毒感染模型構建、免疫調節機制研究及藥物干預效果評估提供定量數據支持，尤其適用于動物實驗中對IFN-β分泌水平的分析，如中樞神經系統炎癥、病毒性肝炎或自身免疫性疾病等研究場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Ifnb1 ELISA Kit; IfnbInterferon beta ELISA Kit; IFN-beta ELISA Kit
縮寫：

IFNB1
Uniprot No.：

P70499
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-102
1:2	Average %	104
	Range %	98-110
1:4	Average %	90
	Range %	85-94
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of pig LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	87	80-91

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Has antiviral, antibacterial and anticancer activities.
基因功能參考文獻：
1. results provided evidence that delivery of IL-18 and IFN-beta by BMSCs may be an excellent and promising approach to develop an effective treatment protocol for glioma therapy. PMID: 26252165
2. IFN-gamma is able to activate rat intestinal mucous microvascular endothelial cells, thereby leading to upregulated expressions of IFN-alpha/beta. PMID: 20214528
3. These findings validate IFN-beta as a therapeutic molecule for intraarticular gene therapy of arthritis. PMID: 16984225
4. This study presents novel effects of IFN-beta in the central nervous system and begins to unravel the mechanism behind the known excitability changes in neurons. PMID: 19541371
亞細胞定位：

Secreted.
蛋白家族：

Alpha/beta interferon family
數據庫鏈接：

KEGG: rno:24481

STRING: 10116.ENSRNOP00000008225

UniGene: Rn.138105