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  4. Rat Immunoglobulin G,IgG ELISA Kit

Rat Immunoglobulin G,IgG ELISA Kit

  • 中文名稱：
    大鼠免疫球蛋白G(IgG)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07981r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠免疫球蛋白G(IgG)酶聯(lián)免疫試劑盒（CSB-E07981r）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IgG含量。IgG即免疫球蛋白G，是人體內(nèi)最為豐富的抗體類型。它在免疫防御中至關(guān)重要，能識別并結(jié)合病原體。研究機制主要圍繞其結(jié)構(gòu)和功能展開，解析其與抗原的結(jié)合模式，通過改造IgG結(jié)構(gòu)以提升免疫治療效果，應(yīng)用于腫瘤、自身免疫病等領(lǐng)域。試劑盒檢測范圍為31.25 ng/mL-8000 ng/mL，靈敏度為31.25 ng/mL。適用于免疫學(xué)研究中評估大鼠模型體液免疫水平，例如感染性疾病、疫苗效力測試、自身免疫疾病或藥物干預(yù)實驗中的抗體應(yīng)答監(jiān)測，也可用于生物制劑研發(fā)中抗體濃度測定本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    IgG
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    31.25 ng/mL-8000 ng/mL
  • 靈敏度：
    31.25 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1000 Average % 100  
    Range % 95-104  
    1:2000 Average % 90  
    Range % 86-95  
    1:4000 Average % 87  
    Range % 83-92  
    1:8000 Average % 101  
    Range % 97-106  
  • 回收率：
    The recovery of rat IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 88-95  
    EDTA plasma (n=4) 93 89-97  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    8000 0.230 0.225 0.228    
    2000 0.417 0.407 0.412    
    500 0.643 0.664 0.654    
    125 1.238 1.257 1.248    
    31.25 1.754 1.770 1.762    
    0 2.035 1.988 2.012    
  • 本試劑盒所含材料：
      • A 96-well Assay plate --The 96-well plate has been pre-coated with rat IgG.
      • Standard (6 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • HRP-conjugated IgG(1 x 6 ml) --Bind to the IgG, and HRP catalyzes the TMB to elicit a chromogenic reaction.
      • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
      • Wash Buffer (25x concentrate) 1 x 20 ml --Wash away unbound or free substances.
      • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

靶點詳情

  • 最新研究進展：
    IgG是一種免疫球蛋白，是人體免疫系統(tǒng)中最常見的抗體類型。它可以識別和結(jié)合到外來物質(zhì)，如病毒、細菌和其他病原體，從而幫助身體清除這些病原體。最近的研究表明，IgG不僅可以發(fā)揮免疫作用，還可以在其他方面發(fā)揮作用，如參與血小板功能調(diào)節(jié)、影響血腦屏障通透性、調(diào)節(jié)神經(jīng)元發(fā)育等。此外，人體在某些疾病或免疫系統(tǒng)失調(diào)狀態(tài)下，會產(chǎn)生抗IgG抗體，這些抗體會與IgG結(jié)合并影響其功能，相關(guān)研究也得到了不斷的深入。