在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Rat Immunoglobulin E,IgE ELISA Kit

  • 中文名稱:
    大鼠免疫球蛋白E(IgE)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E07984r
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠免疫球蛋白E(IgE)酶聯(lián)免疫試劑盒(CSB-E07984r)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿樣本中的IgE含量。IgE即免疫球蛋白 E,是參與過敏性疾病發(fā)生發(fā)展的重要靶點(diǎn)。其背景在于它與速發(fā)型過敏反應(yīng)密切相關(guān)。研究機(jī)制主要是,IgE 可與肥大細(xì)胞、嗜堿性粒細(xì)胞表面受體結(jié)合,當(dāng)變應(yīng)原再次入侵,引發(fā) IgE 交聯(lián),促使細(xì)胞釋放炎性介質(zhì),從而導(dǎo)致過敏癥狀。試劑盒檢測(cè)范圍為3.12 ng/mL-200 ng/mL,適用于過敏機(jī)制研究、寄生蟲感染模型評(píng)估、免疫調(diào)節(jié)藥物篩選等科研領(lǐng)域,為探索Th2型免疫反應(yīng)、肥大細(xì)胞激活等研究提供可靠工具本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    IgE
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma
  • 檢測(cè)范圍:
    3.12 ng/mL-200 ng/mL
  • 靈敏度:
    0.78 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %85
    Range %84-93
    1:2Average %96
    Range %91-103
    1:4Average %84
    Range %81-89
    1:8Average %93
    Range %89-97
  • 回收率:
    The recovery of rat IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-98
    EDTA plasma (n=4)9590-99
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2002.055 2.086 2.071 1.926
    1001.520 1.649 1.585 1.440
    501.101 1.156 1.129 0.984
    250.738 0.750 0.744 0.599
    12.50.417 0.445 0.431 0.286
    6.250.341 0.337 0.339 0.194
    3.120.201 0.213 0.207 0.062
    00.139 0.150 0.145  
  • 本試劑盒所含材料:
    • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat IgE antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled IgE antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
    • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
    • One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
    • An instruction manual

    顯示更多

    收起更多

  • 本試劑盒不含材料:
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

    顯示更多

    收起更多

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)



主站蜘蛛池模板: 欧美精品成人v高清视频| 久久99精品久久久久久| 中国女人内射6xxxxx| 一个色综合国产色综合| 国产公开久久人人97超碰| 777亚洲精品乱码久久久久久| 天天综合网网欲色| 在线视频精品免费观看10| 婷婷色综合视频在线观看| 精品一区二区三区四区五区六区| 午夜无码一区二区三区在线观看| 狠狠做深爱婷婷丁香综合| 性色av一二三天美传媒| 久久久噜噜噜www成人网| 成人午夜视频精品一区| 久久 国产 尿 小便 嘘嘘| 天堂资源在线官网| 性少妇中国内射xxxx狠干| 成人av片无码免费网站| 亚洲日韩在线a视频在线观看| 国产精品偷伦视频观看免费| 免费又黄又爽又色的视频| 欧美乱大交xxxxx潮喷| 99re久久热在这里精品| 午夜精品乱人伦小说区| 国产三级精品三级在专区| 天天做av天天爱天天爽| 浪货趴办公桌~h揉秘书电影| 国产成人精品无码免费看夜聊软件| 天堂а√在线官网| 国产无遮挡又黄又爽动态图| 亚洲 a v无 码免 费 成 人 a v | 成年丰满熟妇午夜免费视频| 久久精品国产亚洲夜色av网站| 蜜桃麻豆www久久国产sex| 成人综合婷婷国产精品久久蜜臀 | 99亚洲男女激情在线观看| 亚洲人成网站在线播放影院在线| 国产大量精品视频网站| 秋霞特色aa大片| 久久er热在这里只有精品66|