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  4. Rat IL-1 Receptor Like 1(IL1RL1)ELISA Kit

Rat IL-1 Receptor Like 1(IL1RL1)ELISA Kit

  • 中文名稱：
    大鼠白介素1受體樣1(IL1RL1)酶聯免疫試劑盒
  • 貨號：
    CSB-E14896r
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠白介素1受體樣1(IL1RL1)酶聯免疫試劑盒（CSB-E14896r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的IL1RL1含量。IL1RL1是一種重要的靶點。它參與免疫調節信號通路，在炎癥反應等生理病理過程中扮演關鍵角色。科研人員聚焦于其與疾病關聯的研究機制，期望通過干預該靶點，研發出針對炎癥性疾病、自身免疫病等的有效治療方案。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于科研領域中對IL1RL1表達動態的探索，例如炎癥性疾病動物模型的機制研究、藥物干預后生物標志物水平變化監測，或心血管疾病相關信號通路的功能解析本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Il1rl1 ELISA Kit; Fit1 ELISA Kit; Interleukin-1 receptor-like 1 ELISA Kit; EC 3.2.2.6 ELISA Kit; Fit-1 ELISA Kit; Fos-responsive gene 1 protein ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL1RL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %97
    Range %91-104
    1:4Average %96
    Range %91-101
    1:8Average %92
    Range %86-96
  • 回收率：
    The recovery of rat IL1RL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9690-100
    EDTA plasma (n=4)9085-95
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.317 2.252 2.285 2.153
    5001.969 1.923 1.946 1.814
    2501.643 1.510 1.577 1.445
    1251.211 1.191 1.201 1.069
    62.50.832 0.784 0.808 0.676
    31.20.554 0.537 0.546 0.414
    15.60.325 0.305 0.315 0.183
    00.136 0.128 0.132 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function.; Inhibits IL-33 signaling.
  • 基因功能參考文獻：
    1. administration of sST2 significantly attenuated the severity of severe acute pancreatitis and associated cardiac damage, and the cardioprotective effect is associated with its anti-inflammatory action PMID: 29653119
    2. Study demonstrates that IL-33 and ST2 are highly expressed by various central nervous system (CNS) resident cells, and there was a change of the expression levels and patterns of both molecules in the CNS lesions of multiple sclerosis (MS)patients compared with healthy brain tissues. Furthermore, IL-33/ST2 signaling pathway may have its unique important function in the myelination process in the CNS compartment. PMID: 27455844
    3. A strong relationship of IL-33/ST2 with natriuretic peptides and classical inflammatory mediators was observed in cardiac tissue. Expression of sST2 in cardiac, adipose and liver tissue decreased in obese rats compared with controls, suggesting an involvement for IL-33/ST2 system in molecular mechanisms of obesity. PMID: 28274612
    4. these results demonstrated that ST2L translocation from the cytosol to the cell membranes of lung tissue and the down-expression of sST2 in both fractions can function as new biomarkers of (ventilator-induced lung injury. PMID: 25815839
    5. This is the first study demonstrating a deleterious role for sST2 in the vascular remodeling associated with obesity. PMID: 24265755
    6. Interleukin-33 prevents apoptosis and improves survival after experimental myocardial infarction through ST2 signaling. PMID: 19919994

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  • 亞細胞定位：
    Cell membrane; Single-pass type I membrane protein.; [Isoform B]: Secreted.
  • 蛋白家族：
    Interleukin-1 receptor family
  • 組織特異性：
    Isoform A is detected in spleen, lung, bone marrow and lymh node. Isoform B is predominant in fibroblasts.
  • 數據庫鏈接：


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