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中文名稱:大鼠透明質酸(HA)酶聯免疫試劑盒
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貨號:CSB-E08120r
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規格:96T/48T
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價格:¥3900/¥2500
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其他:
產品詳情
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產品描述:大鼠透明質酸(HA)酶聯免疫試劑盒(CSB-E08120r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的HA含量。靶點研究是醫藥創新的關鍵環節,通過深入疾病分子機制和生物學特性,尋找新的藥物靶點,設計精確治療方案。研究機制包括基因組學、蛋白質組學和生物信息學,以及高通量篩選技術,旨在發現和開發高效、選擇性的藥物。試劑盒檢測范圍為4.69 ng/mL-300 ng/mL,適用于探索HA在組織再生、慢性炎癥或代謝性疾病中的動態變化,如通過檢測肝病模型中的血清HA水平分析纖維化進展,或評估關節炎動物模型中滑膜組織勻漿的HA代謝異常。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:N/A
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縮寫:HA
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:4.69 ng/mL-300 ng/mL
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靈敏度:1.17 ng/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Cell Biology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 90-96 1:2 Average % 91 Range % 87-96 1:4 Average % 97 Range % 93-100 1:8 Average % 89 Range % 85-96 -
回收率:
The recovery of rat HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 90-96 EDTA plasma (n=4) 95 90-101 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 300 2.656 2.678 2.667 2.505 150 1.848 1.892 1.870 1.708 75 1.202 1.264 1.233 1.071 37.5 0.763 0.778 0.771 0.609 18.75 0.496 0.473 0.485 0.323 9.38 0.384 0.371 0.378 0.216 4.69 0.275 0.298 0.287 0.125 0 0.161 0.163 0.162 -
數據處理:
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貨期:3-5 working days
引用文獻
- Chitosan tamarind-based nanoparticles as a promising approach for topical application of curcumin intended for burn healing: in vitro and in vivo study ES Shalaby,Journal of drug targeting,2023
- Effects of novel Fufang Biejia Ruangan Tablets with sheep placenta as substitute for Hominis Placenta on CCl4-induced liver fibrosis B Shen,Chinese Herbal Medicines,2023
- In vivo assessment of the durable, green and in situ bio-functional cotton fabrics based carboxymethyl chitosan nanohybrid for wound healing application TI Shaheen,International journal of biological macromolecules,2023
- Calcium dobesilate prevents PLD-induced hand-foot syndrome by alleviating capillary endothelial tight junction injury via the HA/CD44 pathway C Li,American journal of cancer research,2023
- Cold plasma approach fortifies the topical application of thymoquinone intended for wound healing via up-regulating the levels of TGF-?, VEGF, and α-SMA in rats AA Sedik,International immunopharmacology,2023
- A standardized pomegranate fruit extract ameliorates thioacetamide-induced liver fibrosis in rats via AGE-RAGE-ROS signaling HM Abouelezz,Heliyon,2023
- Ischemic Preconditioning Alleviates Cerebral Ischemia–Reperfusion Injury by Interfering With Glycocalyx YN Zhang,Translational Stroke Research,2022
- Comprehensive analysis of transcriptomics and metabolomics to illustrate the underlying mechanism of helenalin against hepatic fibrosis Yuhua Xiong,European Journal of Pharmacology,2022
- Mesenchymal stem cell-based Smad7 gene therapy for experimental liver cirrhosis DN Su,Stem Cell Research & Therapy,2020
- Sidr honey abrogates the oxidative stress and downregulates the hyaluronic acid concentration and gene expression of TGF‐β1 and COL1a1 in rat model of thioacetamide‐induced hepatic fibrosis MM Zeweil,Animal Science,2020
- The application of Compont gel in chronic obstructive jaundice rats model Luo WW,ACTA CIRURGICA BRASILEIRA,2019
- The liver sinusoidal endothelial cell damage in rats caused by heatstroke Xingqin Zhang.et al,European Journal of Inflammation,2018
- Hepatoprotective effects of Dicliptera chinensis polysaccharides on dimethylnitrosamine-induced hepatic fibrosis rats and its underlying mechanism Zhang K. et al,J Ethnopharmacol,2015
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