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  4. Rat Glucagon,GC ELISA Kit

Rat Glucagon,GC ELISA Kit

  • 中文名稱：
    大鼠胰高血糖素(GC)酶聯免疫試劑盒
  • 貨號：
    CSB-E12800r
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠胰高血糖素(GC)酶聯免疫試劑盒（CSB-E12800r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GCG含量。GCG是人體中能編碼胰高血糖素等激素的基因。其編碼產物在維持血糖穩態等方面發揮關鍵作用，當GCG功能異常會影響血糖調節。研究上主要聚焦其信號通路、與疾病關聯機制，期望以此為靶點開發治療糖尿病、肥胖癥等代謝疾病藥物。試劑盒檢測范圍為0.78 pg/mL-50 pg/mL，可廣泛用于動物模型研究、代謝調控機制探索、藥物干預效果評估等科研領域。樣本兼容性覆蓋血液（血清/血漿）及組織提取物，為多維度實驗設計提供支持，例如探究不同生理狀態下胰高血糖素分泌動態或器官特異性表達差異。助力研究人員深入解析GC在能量代謝網絡中的功能及病理生理學意義。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    GcgGlucagon [Cleaved into: Glicentin; Glicentin-related polypeptide ELISA kit; GRPP); Oxyntomodulin ELISA kit; OXM ELISA kit; OXY); Glucagon; Glucagon-like peptide 1 ELISA kit; GLP-1); Glucagon-like peptide 1(7-37 ELISA kit; GLP-1(7-37)); Glucagon-like peptide 1(7-36 ELISA kit; GLP-1(7-36)); Glucagon-like peptide 2 ELISA kit; GLP-2)] ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.78 pg/mL-50 pg/mL
  • 靈敏度：
    0.195 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat GC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %92
    Range %88-95
    1:200Average %103
    Range %99-106
    1:400Average %93
    Range %89-97
    1:800Average %100
    Range %95-103
  • 回收率：
    The recovery of rat GC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9491-97
    EDTA plasma (n=4)9491-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    502.035 2.022 2.029 1.890
    251.330 1.321 1.326 1.187
    12.50.904 0.892 0.898 0.759
    6.250.554 0.562 0.558 0.419
    3.120.360 0.356 0.358 0.219
    1.560.278 0.287 0.283 0.144
    0.780.239 0.229 0.234 0.095
    00.141 0.137 0.139 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.; Potent stimulator of glucose-dependent insulin release (Probable). Also stimulates insulin release in response to IL6. Plays important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Has growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis (Probable).; Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.; May modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.; Oxyntomodulin significantly reduces food intake.
  • 亞細胞定位：
    Secreted.; [Glucagon-like peptide 1]: Secreted.
  • 蛋白家族：
    Glucagon family
  • 組織特異性：
    Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract.
  • 數據庫鏈接：


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