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  4. Rat Follistatin,FS ELISA Kit

Rat Follistatin,FS ELISA Kit

  • 中文名稱：
    大鼠卵泡抑素(FS)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08507r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠卵泡抑素(FS)酶聯(lián)免疫試劑盒（CSB-E08507r）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的FST含量。試劑盒檢測(cè)范圍為0.2 ng/mL-40 ng/mL，適用于卵巢功能研究、生殖內(nèi)分泌調(diào)控機(jī)制探索、疾病模型FS表達(dá)動(dòng)態(tài)分析等科研領(lǐng)域。通過(guò)優(yōu)化樣本預(yù)處理流程，可兼容高脂血漿及多種組織勻漿的檢測(cè)需求，為體外實(shí)驗(yàn)提供穩(wěn)定可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    FstFollistatin ELISA Kit; FS ELISA Kit; Activin-binding protein ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.2 ng/mL-40 ng/mL
  • 靈敏度：
    0.1 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat FS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %84
    Range %81-89
    1:2Average %95
    Range %92-98
    1:4Average %102
    Range %98-106
    1:8Average %89
    Range %84-93
  • 回收率：
    The recovery of rat FS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-95
    EDTA plasma (n=4)8985-93
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    400.167 0.160 0.164
    100.401 0.432 0.417
    3.20.549 0.536 0.543
    0.80.951 0.942 0.947
    0.21.485 1.478 1.482
    02.548 2.409 2.479
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).
  • 基因功能參考文獻(xiàn)：
    1. These data suggest that ghrelin inhibits myocardial fibrosis, attenuates left ventricular remodeling, and eventually improves cardiac function by adjusting Act A/FS imbalance. PMID: 29113826
    2. manifestation of polycystic ovarian syndrome-like phenotype following prenatal exposure to excess androgen is associated with irregularity in expression of the FST gene during the estrus cycle PMID: 28277126
    3. Results show that Myostatin and follistatin protein expression is reduced in the long-term hypertrophied myocardium from spontaneously hypertensive rats with heart failure. PMID: 27128567
    4. Myogenin, myostatin, and follistatin expression is lower and myogenic regulatory factor (MRF)4 levels higher in spontaneously hypertensive rats (SHR) with heart failure compared to the Wistar-Kyoto strain. PMID: 22464481
    5. Expression of follistatin and myostatin mRNA is increased in muscle and subcutaneous fat of sedentary diabetic rats compared with sedentary control rats. PMID: 22332899
    6. The ratio of follistatin/myostatin could be used to monitor the progress of target muscle atrophy and recovery. PMID: 22033906
    7. Fst expression is necessary for the proliferation of small hepatocytes. PMID: 21826650
    8. Effects of training and androgens on MYOPRO and FOLLI concentrations in blood and skeletal muscle, were investigated. PMID: 20801187
    9. During chronic heart failure, decreased skeletal muscle trophism is combined with unchanged myostatin and reduced follistatin expression. PMID: 20002838
    10. demonstrate that levels of follistatin mRNA show two surges during the estrous cycle; a relationship between ovarian follistatin expression and progesterone secretion is also suggested PMID: 11906933
    11. PACAP and GnRH down-regulate follicle-stimulating hormone-beta mRNA levels by stimulating follistatin gene expression. PACAP influences FSH production selectively by a paracrine mechanism through folliculostellate cells that involves follistatin. PMID: 12088867
    12. frequency regulation of FSHbeta transcription involves both direct actions of GnRH and indirect effects, via changes in pituitary FS expression PMID: 12193535
    13. we studied the expression of follistatin in rat and mouse liver tumors as a possible mechanism to overcome activin growth control, 40% of the tumors displayed increased levels of follistatin mRNA when compared to tumor-surrounding liver tissue. PMID: 12203361
    14. follistatin originating from anterior pituitary folliculostellate cells participates in mediating the effects of IL-1beta, glucocorticoids, and other agents on the response of pituitary cells to activins. PMID: 12538636
    15. crystal structure of the presumed heparan sulfate-binding domain of follistatin, both alone and in complex with the small heparin analogs sucrose octasulfate and D-myo-inositol hexasulfate PMID: 12867435
    16. inhibin is a major regulator of FS, both by transcriptional and nontranscriptional mechanisms. PMID: 14561646
    17. Data suggest that betaA- and betaC-activin subunit proteins and follistatin are autocrine growth regulators in regenerating liver and when expressed independently lead to hepatocyte apoptosis or mitosis in a subset of hepatocytes. PMID: 15821113
    18. The roles of activin A and follistatin in hepatic fribrogenesis are reported. PMID: 16123203
    19. Follistatin was overexpressed while both activin subunits were downregulated in the majority of rat and human liver tumours PMID: 16935389
    20. Follistatin mRNA levels increased significantly with GnRH treatment in young animals but were not significantly changed in the middle aged females. PMID: 17344471
    21. intron 1 of the follistatin gene has a critical role in mediating Smad-dependent effects of activin and regulating the expression level of this gene PMID: 18184649
    22. Activin betaA subunit, follistatin and follistatin-like 3 are expressed in the endometrium of ovariectomized rats and regulated by estrogen replacement PMID: 18781389
    23. Fst expression in the pineal gland exhibits a ~20-fold increase at night, reaching levels that are > 16 fold higher than in other tissues. Expression is controlled by an adrenergic-cyclic AMP mechanism. PMID: 19103603

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  • 亞細(xì)胞定位：
    Secreted.
  • 數(shù)據(jù)庫(kù)鏈接：


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