Rat Folic acid,FA ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠葉酸(FA)酶聯(lián)免疫試劑盒（CSB-E08757r）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的FA含量。葉酸是一種水溶性B族維生素，對(duì)DNA合成、修復(fù)和甲基化至關(guān)重要。在大鼠研究中，葉酸缺乏會(huì)影響生長發(fā)育，可能通過調(diào)節(jié)胰島素樣生長因子（IGFs）系統(tǒng)的甲基化水平，進(jìn)而影響胚胎發(fā)育和細(xì)胞增殖。葉酸還與DNA甲基化、細(xì)胞代謝和神經(jīng)管缺陷等過程密切相關(guān)。試劑盒檢測范圍為19.5 pg/mL-5000 pg/mL，靈敏度為19.5 pg/mL。本試劑盒適用于科研領(lǐng)域中對(duì)大鼠葉酸代謝相關(guān)研究，包括膳食營養(yǎng)評(píng)估、葉酸缺乏模型構(gòu)建、藥物干預(yù)效果分析等基礎(chǔ)實(shí)驗(yàn)，為探索葉酸在生長發(fā)育、造血功能及神經(jīng)系統(tǒng)中的作用機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

FA
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma
檢測范圍：

19.5 pg/mL-5000 pg/mL
靈敏度：

19.5 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat folic acid in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	86-104
1:2	Average %	92
	Range %	83-99
1:4	Average %	96
	Range %	87-98
1:8	Average %	94
	Range %	80-98

回收率：

The recovery of rat folic acid spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	86-102
EDTA plasma (n=4)	94	88-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究進(jìn)展：

葉酸（Folic acid）是一種維生素B族成員，參與人體DNA、RNA的合成和修復(fù)，對(duì)于細(xì)胞生長和分裂非常重要。最新的研究表明，葉酸在人體內(nèi)具有多種生理功能，包括對(duì)于心血管健康和神經(jīng)系統(tǒng)健康的影響，以及對(duì)于出生缺陷和某些類型的癌癥的預(yù)防作用。