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  4. Rat Epithelial neutrophil activating peptide 78,ENA-78 ELISA Kit

Rat Epithelial neutrophil activating peptide 78,ENA-78 ELISA Kit

  • 中文名稱：
    大鼠上皮中性粒細(xì)胞活化肽78(ENA-78/CXCL5)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08179r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠上皮中性粒細(xì)胞活化肽78(ENA-78/CXCL5)酶聯(lián)免疫試劑盒（CSB-E08179r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CXCL5含量。CXCL5 屬于趨化因子，在炎癥反應(yīng)、腫瘤發(fā)展等過(guò)程中有重要作用。其研究機(jī)制主要涉及與受體結(jié)合，激活下游信號(hào)通路，促進(jìn)細(xì)胞遷移、增殖等。在腫瘤領(lǐng)域，它可影響腫瘤微環(huán)境，促進(jìn)血管生成，為相關(guān)疾病治療提供潛在靶點(diǎn)。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，靈敏度為0.078 ng/mL。本試劑盒適用于科研領(lǐng)域中對(duì)CXCL5在炎癥模型、免疫調(diào)節(jié)或疾病機(jī)制研究中的動(dòng)態(tài)變化分析，如通過(guò)血清或血漿樣本評(píng)估系統(tǒng)性炎癥狀態(tài)，或利用組織勻漿探究局部CXCL5表達(dá)特征，為探索相關(guān)信號(hào)通路、藥物干預(yù)效果等提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    Cxcl5 ELISA Kit; Scyb5C-X-C motif chemokine 5 ELISA Kit; Cytokine LIX ELISA Kit; Small-inducible cytokine B5 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(ng/ml)

    2.442

    2.474

    2.529

    2.446

    2.487

    2.542

    SD

    0.035

    0.033

    0.038

    0.041

    0.051

    0.048

    CV(%)

    5.486

    5.116

    5.784

    6.416

    7.870

    7.273

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat ENA-78/CXCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    88

    Range %

    83-91

    1:2

    Average %

    104

    Range %

    98-108

    1:4

    Average %

    92

    Range %

    87-96

    1:8

    Average %

    96

    Range %

    91-99

  • 回收率：

    The recovery of rat ENA-78/CXCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    90

    84-94

    EDTA plasma (n=4)

    94

    87-97

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    20

    2.746

    2.689

    2.718

    2.607

    10

    2.000

    1.899

    1.950

    1.839

    5

    1.190

    1.171

    1.181

    1.070

    2.5

    0.651

    0.640

    0.646

    0.535

    1.25

    0.366

    0.354

    0.360

    0.249

    0.625

    0.263

    0.257

    0.260

    0.149

    0.312

    0.172

    0.178

    0.175

    0.064

    0.064

    0

    0.110

    0.112

    0.111

    　

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    CXCL5，也稱為ENA-78，是一種重要的趨化因子蛋白，可誘導(dǎo)中性粒細(xì)胞和其他白細(xì)胞向感染或炎癥部位遷移。最近的研究表明，CXCL5在多種腫瘤中的表達(dá)與惡性程度和預(yù)后密切相關(guān)，并且可作為潛在的治療靶點(diǎn)。
  • 功能：
    May participate in the recruitment of inflammatory cells by injured or infected tissue.
  • 基因功能參考文獻(xiàn)：
    1. CRH suppressed CXCL5 production in glia via CRHR1 in the hippocampus of male rats. PMID: 29126185
    2. up-regulation of spinal CXCL5 and CXCR2 is involved in neuropathic pain after nerve injury, through regulating GSK-3beta activity in rats. PMID: 27717828
    3. Analysis of mitogen-activated protein kinases (MAPK) and glycogen synthase kinase (GSK3) pathways showed that they are involved in mechanisms of neuronal apoptosis in response to the depletion of CXCL5/LIX signaling. PMID: 22034072
    4. Data demonstrate that the chemokine CXCL5 is a peripheral mediator of UVB-induced inflammatory pain, likely in humans as well as rats. PMID: 21734176
    5. Disrupted expression of CXCL5 in colorectal cancer is associated with rapid tumor formation in rats and poor prognosis in patients. PMID: 18413816

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine alpha (chemokine CxC) family
  • 數(shù)據(jù)庫(kù)鏈接：


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