在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Rat Cystatin C,Cys-C ELISA Kit

Rat Cystatin C,Cys-C ELISA Kit

  • 中文名稱：
    大鼠半胱氨酸蛋白酶抑制劑/胱抑素C(Cys-C)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08385r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠半胱氨酸蛋白酶抑制劑/胱抑素C(Cys-C)酶聯(lián)免疫試劑盒（CSB-E08385r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、勻漿樣本中的CST3含量。CST3 即半胱氨酸蛋白酶抑制劑 C，是一種內源性半胱氨酸蛋白酶抑制劑。在人體生理中起穩(wěn)定蛋白酶平衡等作用。研究發(fā)現(xiàn)其與神經系統(tǒng)疾病、腎臟疾病等密切相關，如可作為評估腎小球濾過功能指標，機制可能與調節(jié)體內蛋白酶活性和炎癥反應等有關。試劑盒檢測范圍為0.312 ng/ml-20 ng/ml，靈敏度為0.078 ng/ml。適用于科研領域中對腎臟疾病模型、藥物代謝研究或組織損傷相關實驗中Cys-C表達水平的動態(tài)監(jiān)測，幫助研究者分析其與腎功能狀態(tài)、炎癥進程或細胞凋亡的關聯(lián)性。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 別名：
    Cst3 ELISA Kit; Cystatin-C ELISA Kit; Cystatin-3 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, homogenates
  • 檢測范圍：
    0.312 ng/ml-20 ng/ml
  • 靈敏度：
    0.078 ng/ml
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(ng/ml)

    2.490

    2.550

    2.588

    2.467

    2.527

    2.555

    SD

    0.031

    0.039

    0.046

    0.041

    0.048

    0.056

    CV(%)

    3.407

    4.225

    4.941

    4.530

    5.229

    6.061

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat Cys-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:2

    Average %

    96

    Range %

    93-99

    1:4

    Average %

    93

    Range %

    90-96

    1:8

    Average %

    96

    Range %

    91-104

    1:16

    Average %

    92

    Range %

    89-95

  • 回收率：

    The recovery of rat Cys-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    93

    89-97

    EDTA plasma (n=4)

    94

    90-98

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    C:UsersAdministratorAppDataLocalTemp企業(yè)微信截圖_17157650865552.png

    ng/ml

    OD1

    OD2

    Average

    Corrected

    20

    2.564

    2.587

    2.576

    2.404

    10

    1.923

    1.934

    1.929

    1.757

    5

    1.329

    1.368

    1.349

    1.177

    2.5

    0.912

    0.937

    0.925

    0.753

    1.25

    0.582

    0.598

    0.590

    0.418

    0.625

    0.412

    0.436

    0.424

    0.252

    0.312

    0.311

    0.317

    0.349

    0.177

    0

    0.170

    0.174

    0.172

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 最新研究進展：
    CST3是一種由人體細胞產生的小分子蛋白質，也被稱為半胱氨酸蛋白酶抑制劑或淀粉樣蛋白沉積物抑制劑。它在細胞凋亡、免疫調節(jié)、組織修復和生殖系統(tǒng)發(fā)育等方面發(fā)揮重要作用。最近的研究表明，CST3與多種疾病有關，包括神經退行性疾病、腎臟疾病和腫瘤。
     
  • 功能：
    As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L.
  • 基因功能參考文獻：
    1. Tanshinone IIA protects against cardiac hypertrophy in spontaneously hypertensive rats by inhibiting the Cys-C/Wnt signaling pathway. PMID: 28053285
    2. These results indicate that Cd increases the urinary excretion of cystatin C, and they suggest that this effect may involve disruption of megalin-mediated uptake of cystatin C by epithelial cells of the proximal tubule. PMID: 26715107
    3. High cystatin C is associated with proteinuria. PMID: 21551085
    4. Triiodothyronine-induced increase in the production of cystatin C may be related to an increased cell metabolism and proteolysis control demand. PMID: 22360852
    5. CysC plays a protective role under conditions of neuronal challenge by inducing autophagy via mTOR inhibition PMID: 20352108
    6. Oxidants induce elevated cystatin C production from cardiac myocytes. Cystatin C plays a role in cardiac extracellular matrix remodelling. PMID: 20489058
    7. We found several novel glycoforms of cystatin c in rat hippocampal stem cells, suggesting that N- and O-linked glycans with sialic acid are attached to cystatin C. PMID: 15197734
    8. These results demonstrate that cystatin C has protective effects against various oxidative stresses that induce cell death. PMID: 16140167
    9. endometrial expression of the cysteine protease inhibitor, Cst3, is modulated by estrogen during the peri-implantation period PMID: 16935259
    10. These studies suggest alternate pathways and potential therapeutic targets of cell death associated with oxidative stress, CC, and lysosomal dysregulation. PMID: 17241700
    11. Finally, in vivo studies using kidney-specific megalin knockout mice provide evidence that megalin mediates proximal tubular uptake of cystatin C. We conclude that megalin is an endocytic receptor of cystatin C in PTC. PMID: 17462596

    顯示更多

    收起更多

  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Cystatin family
  • 數(shù)據(jù)庫鏈接：


主站蜘蛛池模板： 国产精品无码av无码| 欧美日产国产新一区| 99精品热在线在线观看视频| 伊人久久大香线蕉综合bd高清| 欧美性猛少妇xxxxx免费| 天天综合色天天综合色hd| 欧美精品在线观看| 荫道bbwbbb高潮潮喷| 丰满熟妇人妻中文字幕| 四虎国产精品永久免费网址| 亚洲丁香婷婷综合久久| 久久精品青青草原伊人| 精品人妻av区波多野结衣| 亚洲成av人在线视| 男人扒开女人双腿猛进视频| 中国农村妇女真实bbwbbwbbw| 无码熟熟妇丰满人妻porn| 人人妻人人澡人人爽久久av| 边添小泬边狠狠躁视频| av网站免费线看精品| 韩国久久久久久级做爰片| 午夜嘿嘿嘿影院| 波多野美乳人妻hd电影欧美| 99久久婷婷国产综合精品电影| 国产精品亚洲w码日韩中文| 成人无码av网站在线观看| 四虎成人精品永久在线视频| 中文字幕无码精品亚洲35| 妺妺窝人体色777777| av片日韩一区二区三区在线观看| 熟妇高潮精品一区二区三区| 精品无码一区二区三区| 精品人妻中文字幕有码在线| 窝窝午夜看片成人精品| 男人狂躁进女人下面免费视频| 麻麻张开腿让我爽了一夜| 国语做受对白xxxxx在线| 无码人妻一区二区三区免费n鬼逝 好爽好湿好硬好大免费视频 | 蜜桃视频在线观看免费网址入口| 国产特级毛片aaaaaa高清| 竹菊影视欧美日韩一区二区三区四区五区 |