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  4. Rat Complement fragment 5,C5 ELISA Kit

Rat Complement fragment 5,C5 ELISA Kit

  • 中文名稱：
    大鼠補體片斷5(C5)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14363r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠補體片斷5(C5)酶聯(lián)免疫試劑盒（CSB-E14363r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C5含量。C5是補體系統(tǒng)中的關鍵成分，在免疫防御中起重要作用。異常激活會引發(fā)多種疾病。研究機制主要圍繞抑制其激活，以減少炎癥反應和組織損傷。目前針對C5的治療手段，如單抗藥物，在多種病癥中展現(xiàn)出良好應用前景。試劑盒檢測范圍為9.38 ng/mL-600 ng/mL，可應用于大鼠模型中的補體系統(tǒng)功能研究，例如評估炎癥性疾病動物模型的病理進程、藥物干預對補體激活的影響，或探究組織損傷修復過程中C5的動態(tài)變化，為免疫學、藥理學及基礎醫(yī)學研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    C5Complement C5 [Cleaved into: C5a anaphylatoxin] ELISA kit; Fragment ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    9.38 ng/mL-600 ng/mL
  • 靈敏度：
    2.34 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat C5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:200

    Average %

    104

    Range %

    97-111

    1:400

    Average %

    101

    Range %

    94-108

    1:800

    Average %

    98

    Range %

    93-103

    1:1600

    Average %

    91

    Range %

    84-97

  • 回收率：

    The recovery of rat C5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    104

    99-110

    EDTA plasma (n=4)

    95

    88-101

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    600

    2.218

    2.134

    2.176

    2.070

    300

    1.864

    1.767

    1.815

    1.710

    150

    1.346

    1.298

    1.322

    1.216

    75

    0.937

    0.929

    0.933

    0.827

    37.5

    0.601

    0.611

    0.606

    0.500

    18.75

    0.436

    0.453

    0.445

    0.339

    9.38

    0.302

    0.294

    0.298

    0.192

    0

    0.106

    0.105

    0.106

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.; Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
  • 亞細胞定位：
    Secreted.
  • 數(shù)據(jù)庫鏈接：